Browse > Article
http://dx.doi.org/10.7845/kjm.2018.8006

Draft genome sequence of lytic bacteriophage CP3 infecting anaerobic bacterial pathogen Clostridium perfringens  

Kim, Youngju (Optipharm Inc.)
Ko, Seyoung (Department of Genetic Engineering and Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University)
Yeon, Young Eun (Optipharm Inc.)
Le, Hoa Thi (Department of Genetic Engineering and Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University)
Han, Beom Ku (Optipharm Inc.)
Kim, Hyunil (Optipharm Inc.)
Oh, Chang-Sik (Department of Horticultural Biotechnology, College of Life Sciences, Kyung Hee University)
Kim, Donghyuk (Department of Genetic Engineering and Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University)
Publication Information
Korean Journal of Microbiology / v.54, no.2, 2018 , pp. 149-151 More about this Journal
Abstract
Clostridium perfringens is a Gram-positive, rod-shaped, anaerobic, spore-forming pathogenic bacterium, which belongs to the Clostridiaceae family. C. perfringens causes diseases including food poisoning in vertebrates and intestinal tract of humans. Bacteriophages that can kill target bacteria specifically have been considered as one of control methods for bacterial pathogens. Here, we report a draft genome sequence of the bacteriophage CP3 effective to C. perfringens. The phage genome comprises 52,068 bp with a G + C content of 34.0%. The draft genome has 74 protein-coding genes, 29 of which have predicted functions from BLASTp analysis. Others are conserved proteins with unknown functions. No RNAs were found in the genome.
Keywords
Clostridium perfringens; bacteriophage CP3; draft genome sequence; illumina;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Brettin, T., Davis, J.J., Disz, T., Edwards, R.A., Gerdes, S., Olsen, G.J., Olson, R., Overbeek, R., Parrello, B., Pusch, G.D., et al. 2015. RASTtk: a modular and extensible implementation of the RAST algorithm for building custom annotation pipelines and annotating batches of genomes. Sci. Rep. 5, 8365.   DOI
2 Camacho, C., Coulouris, G., Avagyan, V., Ma, N., Papadopoulos, J., Bealer, K., and Madden, T.L. 2009. BLAST+: architecture and applications. BMC Bioinformatics 10, 421.   DOI
3 Seal, B.S. 2013. Characterization of bacteriophages virulent for Clostridium perfringens and identification of phage lytic enzymes as alternatives to antibiotics for potential control of the bacterium. Poult. Sci. 92, 526-533.   DOI
4 Seal, B.S., Fouts, D.E., Simmons, M., Garrish, J.K., Kuntz, R.L., Woolsey, R., Schegg, K.M., Kropinski, A.M., Ackermann, H.W., and Siragusa, G.R. 2011. Clostridium perfringens bacteriophages PhiCP39O and PhiCP26F: genomic organization and proteomic analysis of the virions. Arch. Virol. 156, 25-35.   DOI
5 Seguritan, V., Alves, N. Jr. Arnoult, M., Raymond, A., Lorimer, D., Burgin, A.B. Jr., Salamon, P., and Segall, A.M. 2012. Artificial neural networks trained to detect viral and phage structural proteins. PLoS Comput. Biol. 8, e1002657.   DOI
6 Volozhantsev, N.V., Oakley, B.B., Morales, C.A., Verevkin, V.V., Bannov, V.A., Krasilnikova, V.M., Popova, A.V., Zhilenkov, E.L., Garrish, J.K., Schegg, K.M., et al. 2012. Molecular characterization of podoviral bacteriophages virulent for Clostridium perfringens and their comparison with members of the Picovirinae. PLoS One 7, e38283.   DOI