Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Reduction of dissolved hydrogen sulfide and mortality of white leg shrimp, Litopenaeus vannamei by Bacillus spp. microorganisms

Bacillus속 미생물의 용존황화수소 저감효과와 흰다리새우(Litopenaeus vannamei)에의 영향

  • Choi, Jun-Ho (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University) ;
  • Lee, Ji-Hoon (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University) ;
  • Park, Jung-Jin (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University) ;
  • Lee, Min-Sun (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University) ;
  • Bae, Jun-Sung (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University) ;
  • Shin, Dong-Hun (Shindong Control Center For Aquatic Animal Diseases) ;
  • Park, Kwan Ha (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University)
  • 최준호 (군산대학교 수산생명의학과) ;
  • 이지훈 (군산대학교 수산생명의학과) ;
  • 박정진 (군산대학교 수산생명의학과) ;
  • 이민선 (군산대학교 수산생명의학과) ;
  • 배준성 (군산대학교 수산생명의학과) ;
  • 신동훈 (신동수산질병관리원) ;
  • 박관하 (군산대학교 수산생명의학과)
  • Received : 2018.05.02
  • Accepted : 2018.06.14
  • Published : 2018.06.30
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Abstract

The utility of Bacillus spp. organisms for reduction of dissolved hydrogen sulfide ($H_2S$) in white leg shrimp (Litopenaeus vannamei) culture was tested with different combinations of Bacillus spp. microorganisms: combination A (B. subtilis + B. licheniformis); combination B (B. licheniformis + B. amyloliquefaciens); combination C (B. subtilis + B. licheniformis + B. amyloliquefaciens). Of these 3 combinations, C was effective in few hours after addition whereas B needed longer time to be effective. The $H_2S-reducing$ effect of combination C was dependent on the amount of microorganisms added to $H_2S-containing$ test solution. Exposure of white leg shrimp to $H_2S$ at 8 mg/L for 7 days led to survival of 80% and 1 mg/L for 14 days it was 82.5%. The survival rate was 97.5% when combination C was simultaneously added to shrimp tanks during $H_2S$ exposure at 1 mg/L for 14 days. It was demonstrated that combination C microorganisms (B. subtilis + B. licheniformis + B. amyloliquefaciens) can reduce dissolved $H_2S$ concentrations, and this effect can be utilized to protect white leg shrimp from $H_2S$ toxicity.

흰다리새우양식에 있어 저면에서 생성된 황화수소($H_2S$)를 Bacillus속 미생물을 이용하여 저감이 가능한지 아래 3가지 균주들의 조합에 대해 시험하였다. 시험에 사용한 3가지 Bacillus속 균주들을 A시험군(B. subtilis + B. licheniformis), B시험군(B. licheniformis +B. amyloliquefaciens) 및 C시험군(B. subtilis + B. licheniformis + B. amyloliquefaciens)으로 구성하였다. 3가지 조합으로 시험균을 배양 한 뒤, 인위적으로 황화수소(3 mg/L)를 폭로하여 용존황화수소를 측정하였다. 그 결과, 시험군C가 용존황화수소를 초기에 저감하는 것을 측정하였다. 또한 시험군C를 배양하여 분리한 균체의 양을 달리하여 황화수소용액에 가했을 때 황화수소 감소능은 첨가한 균체의 양에 비례하여 증가하였다. 그러나 이 조건에서 최대량의 균체를 가하였을 때에도 시험군A 및 B는 황화수소 감소능을 발휘하지 못하였다. 용존황화수소가 흰다리새우에게 미치는 독성을 평가하기 위해, 황화수소(1-8 mg/L)를 폭로(매 1회/1일, 7일간 반복)하여 생존율을 측정한 결과 8 mg/L에서 가장 높은 누적치사율(20%)을 유발하였다. 흰다리새우 사육수에 시험군C(1 g/L)를 48시간 배양한 후 황화수소 1 mg/L에 폭로시키면서 흰다리새우 생존율을 14일간 지속 관찰한 결과 대조군에서 82.5% 시험군C를 가한 시험군에서는 97.5% 수준의 생존율이 관찰되었다. 그러므로 새우양식에서 3종의 Bacillus속 시험군C는 유해한 용존황화수소의 발생을 줄이고 나아가 흰다리새우 생산량의 증대를 가져올 수 있을 것으로 사료된다.

Keywords

References

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