Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Endothelium Independent Effect of Pelargonidin on Vasoconstriction in Rat Aorta

  • Min, Young Sil (Department of Medical Plant Science, College of Scienceand Engineering, Jung Won University) ;
  • Yoon, Hyuk-Jun (Department of Pharmacology, College of Pharmacy, Catholic University of Daegu) ;
  • Je, Hyun Dong (Department of Pharmacology, College of Pharmacy, Catholic University of Daegu) ;
  • Lee, Jong Hyuk (Department of Pharmaceutical Engineering, College of Life and Health Science, Hoseo University) ;
  • Yoo, Seong Su (Department of Pharmacology, College of Pharmacy, Chung-Ang University) ;
  • Shim, Hyun Sub (Department of Pharmacology, College of Pharmacy, Chung-Ang University) ;
  • Lee, Hak Yeong (Department of Pharmacology, College of Pharmacy, Chung-Ang University) ;
  • La, Hyen-Oh (Department of Clinical Pharmacology, College of Pharmacy, Catholic University of Korea) ;
  • Sohn, Uy Dong (Department of Pharmacology, College of Pharmacy, Chung-Ang University)
  • Received : 2017.09.29
  • Accepted : 2017.12.21
  • Published : 2018.07.01
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Abstract

In this study, we investigated the effects of pelargonidin, an anthocyanidin found in many fruits and vegetables, on endothelium-independent vascular contractility to determine the underlying mechanism of relaxation. Isometric contractions of denuded aortic muscles from male rats were recorded, and the data were combined with those obtained in western blot analysis. Pelargonidin significantly inhibited fluoride-, thromboxane A2-, and phorbol ester-induced vascular contractions, regardless of the presence or absence of endothelium, suggesting a direct effect of the compound on vascular smooth muscles via a different pathway. Pelargonidin significantly inhibited the fluoride-dependent increase in the level of myosin phosphatase target subunit 1 (MYPT1) phosphorylation at Thr-855 and the phorbol 12,13-dibutyrate-dependent increase in the level of extracellular signal-regulated kinase (ERK) 1/2 phosphorylation at Thr202/Tyr204, suggesting the inhibition of Rho-kinase and mitogen-activated protein kinase kinase (MEK) activities and subsequent phosphorylation of MYPT1 and ERK1/2. These results suggest that the relaxation effect of pelargonidin on agonist-dependent vascular contractions includes inhibition of Rho-kinase and MEK activities, independent of the endothelial function.

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References

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