Asian-Australasian Journal of Animal Sciences

  • 제31권8호
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  • Pages.1325-1335
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  • 2018
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  • 1011-2367(pISSN)
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  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Effects of dietary spermine supplementation on cell cycle, apoptosis, and amino acid transporters of the thymus and spleen in piglets

  • Cao, Wei (Institute of Animal Nutrition, Sichuan Agricultural University) ;
  • Wu, Xianjian (Institute of Animal Nutrition, Sichuan Agricultural University) ;
  • Jia, Gang (Institute of Animal Nutrition, Sichuan Agricultural University) ;
  • Zhao, Hua (Institute of Animal Nutrition, Sichuan Agricultural University) ;
  • Chen, Xiaoling (Institute of Animal Nutrition, Sichuan Agricultural University) ;
  • Wu, Caimei (Institute of Animal Nutrition, Sichuan Agricultural University) ;
  • Cai, Jingyi (Institute of Animal Nutrition, Sichuan Agricultural University) ;
  • Wang, Jing (Maize Research Institute, Sichuan Agricultural University) ;
  • Liu, Guangmang (Institute of Animal Nutrition, Sichuan Agricultural University)
  • 투고 : 2017.11.03
  • 심사 : 2018.01.24
  • 발행 : 2018.08.01
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초록

Objective: This study investigated whether spermine supplementation could regulate cell cycle, apoptosis, and amino acid transporter-related genes expression in the thymus and spleen of early weaned piglets. Methods: Eighty female piglets were randomly distributed to receive adequate nutrients supplemented with spermine (0.4 mmol/kg body weight/24 h) or to be provided with restricted nourishment supplemented with normal saline for 7 h or 3, 6, or 9 d in pairs. Results: Regardless of administration time, spermine supplementation significantly up-regulated cyclin A2 gene expression but down-regulated p21 and cyclin D3 mRNA levels in the thymus and spleen and reduced cyclin E2 gene expression in the thymus of piglets (p<0.05). Irrespective of the treatment period, the reduced Bax and caspase-3 gene expressions and improved Bcl-2 mRNA level were observed in the thymus and spleen of spermine-administrated piglets (p<0.05). Regardless of supplementation time, spermine intake significantly enhanced the expressions of amino acid transporter-related genes (SLC1A1, SLC1A5, SLC7A1, SLC7A7, and SLC15A1) in both thymus and spleen, as well as SLC7A9 in the spleen of piglets (p<0.05). In addition, extended spermine administration also markedly promoted cell proliferation, depressed apoptosis and modulated amino acid transport (p<0.05), and such effects were the greatest during prolonged spermine supplementation (6 d) compared to the other time periods (p<0.05). Conclusion: Spermine supplementation may regulate cell cycle during the G1/S phase, suppress apoptosis and modulate amino acid transport. A period of 6 d of spermine supplementation is required to produce the optimal effects on nutritional implications.

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참고문헌

  1. Fang T, Liu G, Cao W, et al. Spermine: new insights into the intestinal development and serum antioxidant status of suckling piglets. RSC Adv 2016;6:31323-35. https://doi.org/10.1039/C6RA05361K
  2. Liu G, Fang T, Yan T, et al. Metabolomic strategy for the detection of metabolic effects of spermine supplementation in weaned rats. J Agric Food Chem 2014;62:9035-42. https://doi.org/10.1021/jf500882t
  3. Cao W, Wu X, Jia G, et al. New insights into the role of dietary spermine on inflammation, immune function and related-signalling molecules in the thymus and spleen of piglets. Arch Anim Nutr 2017;71:175-91. https://doi.org/10.1080/1745039X.2017.1314610
  4. Pegg AE. The function of spermine. IUBMB Life 2014;66:8-18. https://doi.org/10.1002/iub.1237
  5. Liu GM, Yan T, Fang TT, et al. Nutrimetabolomic analysis provides new insights into spermine-induced ileum-system alterations for suckling rats. RSC Adv 2015;5:48769-78. https://doi.org/10.1039/C5RA01507C
  6. Kruger A, Vowinckel J, Mulleder M, et al. Tpo1-mediated spermine and spermidine export controls cell cycle delay and times antioxidant protein expression during the oxidative stress response. EMBO Rep 2013;14:1113-9. https://doi.org/10.1038/embor.2013.165
  7. Nitta T, Igarashi K, Yamashita A, Yamamoto M, Yamamoto N. Involvement of polyamines in B cell receptor-mediated apoptosis: spermine functions as a negative modulator. Exp Cell Res 2001;265:174-83. https://doi.org/10.1006/excr.2001.5177
  8. Miller ER, Ullrey DE. The pig as a model for human nutrition. Annu Rev Nutr 1987;7:361-82. https://doi.org/10.1146/annurev.nu.07.070187.002045
  9. Yin J, Ren W, Duan J, et al. Dietary arginine supplementation enhances intestinal expression of SLC7A7 and SLC7A1 and ameliorates growth depression in mycotoxin-challenged pigs. Amino Acids 2014;46:883-92. https://doi.org/10.1007/s00726-013-1643-5
  10. National Research Council (NRC). Nutrient requirements of swine. Washington, DC, USA: National Academy Press; 1998.
  11. Cheng ZB, Li DF, Xing JJ, Guo XY, Li ZJ. Oral administration of spermine advances intestinal maturation in sucking piglets. Anim Sci 2006;82:621-6. https://doi.org/10.1079/ASC200690
  12. Pfaffl MW. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 2001;29:e45. https://doi.org/10.1093/nar/29.9.e45
  13. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 2001;25:402-8. https://doi.org/10.1006/meth.2001.1262
  14. Romain N, Dandrifosse G, Jeusette F, Forget P. Polyamine concentration in rat milk and food, human milk, and infant formulas. Pediatr Res 1992;32:58-63. https://doi.org/10.1203/00006450-199207000-00011
  15. Hwang HC, Clurman BE. Cyclin E in normal and neoplastic cell cycles. Oncogene 2005;24:2776-86. https://doi.org/10.1038/sj.onc.1208613
  16. Bedelbaeva K, Snyder A, Gourevitch D, et al. Lack of p21 expression links cell cycle control and appendage regeneration in mice. Proc Natl Acad Sci USA 2010;107:5845-50. https://doi.org/10.1073/pnas.1000830107
  17. Zhao Z, Liu J, Wang C, et al. MicroRNA-25 regulates small cell lung cancer cell development and cell cycle through cyclin E2. Int J Clin Exp Pathol 2014;7:7726-34.
  18. Sankaran VG, Ludwig LS, Sicinska E, et al. Cyclin D3 coordinates the cell cycle during differentiation to regulate erythrocyte size and number. Genes Dev 2012;26:2075-87. https://doi.org/10.1101/gad.197020.112
  19. Gong D, Ferrell JE Jr. The roles of cyclin A2, B1, and B2 in early and late mitotic events. Mol Biol Cell 2010;21:3149-61. https://doi.org/10.1091/mbc.e10-05-0393
  20. Yang SD, Bai ZL, Zhang F, et al. Levofloxacin increases the effect of serum deprivation on anoikis of rat nucleus pulposus cells via Bax/Bcl-2/caspase-3 pathway. Toxicol Mech Methods 2014;24:688-96. https://doi.org/10.3109/15376516.2014.963772
  21. Loh KP, Huang SH, De Silva R, Tan BK, Zhu YZ. Oxidative stress: apoptosis in neuronal injury. Curr Alzheimer Res 2006;3:327-37. https://doi.org/10.2174/156720506778249515
  22. Wu B, Cui H, Peng X, et al. Dietary nickel chloride induces oxidative stress, apoptosis and alters Bax/Bcl-2 and caspase-3 mRNA expression in the cecal tonsil of broilers. Food Chem Toxicol 2014;63:18-29. https://doi.org/10.1016/j.fct.2013.10.033
  23. Zheng TS, Flavell RA. Divinations and surprises: genetic analysis of caspase function in mice. Exp Cell Res 2000;256:67-73. https://doi.org/10.1006/excr.2000.4841
  24. Antonsson B, Martinou JC. The Bcl-2 protein family. Exp Cell Res 2000;256:50-7. https://doi.org/10.1006/excr.2000.4839
  25. Daniel H, Kottra G. The proton oligopeptide cotransporter family SLC15 in physiology and pharmacology. Pflugers Arch 2004;447:610-8. https://doi.org/10.1007/s00424-003-1101-4
  26. Kristensen AS, Andersen J, Jorgensen TN, et al. SLC6 neurotransmitter transporters: structure, function, and regulation. Pharmacol Rev 2011;63:585-640. https://doi.org/10.1124/pr.108.000869
  27. Roth E. Immune and cell modulation by amino acids. Clin Nutr 2007;26:535-44. https://doi.org/10.1016/j.clnu.2007.05.007
  28. Kanai Y, Clemencon B, Simonin A, et al. The SLC1 high-affinity glutamate and neutral amino acid transporter family. Mol Aspects Med 2013;34:108-20. https://doi.org/10.1016/j.mam.2013.01.001
  29. Verrey F, Closs EI, Wagner CA, et al. CATs and HATs: the SLC7 family of amino acid transporters. Pflugers Arch 2004;447:532-42. https://doi.org/10.1007/s00424-003-1086-z
  30. Fotiadis D, Kanai Y, Palacin M. The SLC3 and SLC7 families of amino acid transporters. Mol Aspects Med 2013;34:139-58. https://doi.org/10.1016/j.mam.2012.10.007

피인용 문헌

  1. Effects of spermine on liver barrier function, amino acid transporters, immune status, and apoptosis in piglets vol.9, pp.20, 2018, https://doi.org/10.1039/c8ra05421e
  2. Digestive abilities, amino acid transporter expression, and metabolism in the intestines of piglets fed with spermine vol.44, pp.5, 2018, https://doi.org/10.1111/jfbc.13167