Journal of Naturopathy (한국자연치유학회지)

Koean Soceity for Naturopathy (한국자연치유학회)
권호 표지

Distribution of Beneficial Bacteria in the Intestines after Enzamin Ingestion of Bacillus subtilis AK Strain Fermentation

Bacillus subtilis AK균 발효액(Enzamin)의 섭취 후 장내 유익세균의 분포조사

  • Received : 2018.09.03
  • Accepted : 2018.09.17
  • Published : 2018.09.30
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Abstract

Purpose: The purpose of this study was to investigate whether intestinal proliferation is promoted in beneficial intestinal bacteria or decreased in harmful bacteria before and after ingesting Bacillus fermentation broth (ENM) for 8 weeks in the 16 subjects. Method: Intestinal bacteria were identified by PCR amplification using specific 16S rRNA primers. Results: The Bifidobacterium gene index(%)(gi%) increased to 58.92% in the control group and 69.53% in the test group after the ingestion of ENM, but there was no significant difference. Lactobacillus gi% increased significantly (49.37% in the control and 66.43% in the test) (p<.029). Clostridium gi% was significantly decreased after treatment (83.16% in the control and 67.76% in the test) (p<.077). Bacteroides gi% increased significantly (12.58% in the control and 20.87% in the test) after ingesting (p<.095). Prevotella gi% increased significantly (7.55% in the control and 17.28% in the test) after ingesting (p<.005). After ingesting, the median bacteria increased significantly in the control (20.06%) and the test (35.88%) (p<.001). Conclusions: After ingestion of the ENM, the number of beneficial bacteria increased and the number of harmful bacteria Clostridium tended to decrease. This suggests that ingestion of the Bacillus fermented beverage ENM has an effect on the proliferation of intestinal bacteria.

목적: 본 연구는 임상대상자 16명에게 Bacillus 발효용액(ENM)을 8주간 섭취시키기 전 후에 임상대상자들의 변에서 주요 목표 장내 유익세균의 증식이 촉진 되는지 및 유해균은 감소하는지를 연구하는 것이 목적이었다. 방법: 장내세균은 16S rRNA 특정 Primer를 이용하여 PCR 증폭기로 동정 검색하였다. 결과: Bifidobacterium속 gene index (%)(=gi%)는 ENM섭취 후에는 대조군이 58.92%, 임상군은 69.53%로 증가하였으나 유의성은 없었다. Lactobacillus 속 지수는 사후에는 대조군이 49.37%, 임상군은 66.43%로 유의성이 있게 증가하였다 (p<.029). Clostridium 속 지수는 사후에는 대조군이 83.16%, 임상군은 67.76%로 유의하게 감소하였다(p<.077). Bacteroides 속 지수는 사후에는 대조군이 12.58%, 임상군은 20.87%로 유의성이 있게 증가하였다(p<.095). Prevotella 속 지수는 사후에는 대조군이 7.55%, 임상군은 17.28%로 유의성이 있게 증가하였다(p<.005). 중간균체는 사후검사의 경우에는 대조군이 20.06%, 임상군은35.88%로 유의성이 있게 증가하였다(p<.001). 결론: Bacillus 발효액(ENM)을 섭취후에는 유익균 수는주로 증가하였고, 유해균인 Clostridium균수는감소하는경향을보였다. 이는 발효음료 ENM의 섭취가 유익한 장내세균의 증식에 영향을 주는 것으로 판단된다.

Keywords

Acknowledgement

We are grateful to Mr. S.J. Kim and Ms. E.H. Yang, Probioticslab R&D Institute. Bioeleven Co., Seoul, Korea. for their technical assistances. This research was supported by the RSW Dongeu Research Institute in Jecheon, Korea.

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