Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Changes in Non-saponin Fatty Acid Content and Increases in Inhibitory Activities of Collagenase and Elastase by Treatment with Saccharomyces cerevisiae of the Supercritical Fluid Extracted Oil of the Adventitious Roots Culture of Wild Mountain Ginseng

초임계 유체로 추출된 산삼 부정 배양근 오일의 효모균 처리에 의한 비사포닌계 지방산 함량 변화 및 Collagenase 및 Elastase 저해 활성 증대

  • Kim, Chul Joong (Research Institute of Biotechnology, HwajinBioCosmetic) ;
  • Shim, Jae Kwon (Research Institute of Biotechnology, HwajinBioCosmetic) ;
  • Kwon, Kyung Cheol (Research Institute of Biotechnology, HwajinBioCosmetic) ;
  • Lim, Jung Dae (Department of Herbal Medicine Resource, Kangwon National University) ;
  • Choi, Seon Kang (Department of Agricultural Life Sciences, Kangwon National University) ;
  • Yu, Chang Yeon (Department of Bio-Resource Sciences, Kangwon National University) ;
  • Lee, Jae Geun (Research Institute of Biotechnology, HwajinBioCosmetic)
  • 김철중 ((주)화진바이오코스메틱) ;
  • 심재권 ((주)화진바이오코스메틱) ;
  • 권경철 ((주)화진바이오코스메틱) ;
  • 임정대 (강원대학교 생약자원개발학과) ;
  • 최선강 (강원대학교 농생명산업학과) ;
  • 유창연 (강원대학교 생물자원과학과) ;
  • 이재근 ((주)화진바이오코스메틱)
  • Received : 2018.02.28
  • Accepted : 2018.04.13
  • Published : 2018.04.30
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Abstract

Background: To obtain useful cosmetic resources, this study aimed to determine the non-saponin fatty acid and inhibitory activities of collagenase and elastase by treatment of Saccharomyces cerevisiae in supercritical fluid extracted oil of the adventitious root culture of wild mountain ginseng. Methods and Results: We performed supercritical fluid extraction at various conditions such as pressure, temperature, time, and use of co-solvents, unlike the n-hexane extraction for the adventitious roots culture of wild mountain ginseng. The non-saponin-fatty acid obtained from the oil of the adventitious roots culture was incresed by treatment with S. cerevisiae. The supercritical fluid extraction was conducted using gas chromatography. Non-saponin-fatty acid content, in the oil of adventitious roots culture of wild mountain ginseng treated with S. cerevisiae for 2 days were three times higher than that in the control. In addition, the oil of the adventitious roots culture treated with S. cerevisiae was investigated for the anti-wrinkle effect by using collagenase and elastase. The oil of adventitious roots culture treated with S. cerevisiae exhibited higher collagenase and elastase inhibitory activities than those in the control. Conclusions: Supercritical fluid extracted oil of the adventitious roots culture of wild mountain ginseng treated with S. cerevisiae was found to have decreased ratio of saturated fatty acids and incresed ratio and content of unsaturated fatty acids increased. Furthermore, it showed anti-wrinkle effects in vitro.

Keywords

References

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