공업화학 (Applied Chemistry for Engineering)

  • 제29권2호
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  • Pages.176-184
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  • 2018
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  • 1225-0112(pISSN)
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  • 2288-4505(eISSN)
한국공업화학회 (The Korean Society of Industrial and Engineering Chemistry)
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금전초 추출물 및 분획물의 항산화 활성 및 세포 보호 효과

Antioxidative and Cellular Protective Effects of Lysimachia christinae Hance Extract and Fractions

  • 김아랑 (서울과학기술대학교 정밀화학과, 나노바이오화장품연구실, 화장품종합기술연구소) ;
  • 정민철 (한성과학고등학교) ;
  • 정혜인 (한성과학고등학교) ;
  • 송동기 (한성과학고등학교) ;
  • 서영빈 (한성과학고등학교) ;
  • 전영희 (한성과학고등학교) ;
  • 박소현 (서울과학기술대학교 정밀화학과, 나노바이오화장품연구실, 화장품종합기술연구소) ;
  • 신혁수 (서울과학기술대학교 정밀화학과, 나노바이오화장품연구실, 화장품종합기술연구소) ;
  • 이상래 (서울과학기술대학교 정밀화학과, 나노바이오화장품연구실, 화장품종합기술연구소) ;
  • 박수남 (서울과학기술대학교 정밀화학과, 나노바이오화장품연구실, 화장품종합기술연구소)
  • Kim, A Rang (Department of Fine Chemistry, Nanobiocosmetic Lab., Cosmetic R&D Center, Seoul National University of Science and Technology) ;
  • Jung, Min Chul (Hansung Science High School) ;
  • Jeong, Hye In (Hansung Science High School) ;
  • Song, Dong Gi (Hansung Science High School) ;
  • Seo, Young Bin (Hansung Science High School) ;
  • Jeon, Young Hee (Hansung Science High School) ;
  • Park, So Hyun (Department of Fine Chemistry, Nanobiocosmetic Lab., Cosmetic R&D Center, Seoul National University of Science and Technology) ;
  • Shin, Hyuk Soo (Department of Fine Chemistry, Nanobiocosmetic Lab., Cosmetic R&D Center, Seoul National University of Science and Technology) ;
  • Lee, Sang Lae (Department of Fine Chemistry, Nanobiocosmetic Lab., Cosmetic R&D Center, Seoul National University of Science and Technology) ;
  • Park, Soo Nam (Department of Fine Chemistry, Nanobiocosmetic Lab., Cosmetic R&D Center, Seoul National University of Science and Technology)
  • 투고 : 2017.11.18
  • 심사 : 2017.12.22
  • 발행 : 2018.04.10
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⟨ 이전 논문 다음 논문 ⟩

초록

본 논문에서는 금전초로부터 50% 에탄올 추출물, 에틸아세테이트 분획 및 아글리콘 분획을 제조하였고 이들의 항산화 활성, 세포 보호 효과 및 성분 분석에 대한 연구를 수행하였다. 항산화능 평가에서 50% 에탄올 추출물, 에틸아세테이트 분획 및 아글리콘 분획의 라디칼 소거 활성($FSC_{50}$)은 각각 146.8, 22.2 및 $27.2{\mu}g/mL$이었고, 총 항산화능($OSC_{50}$)은 29.3, 2.9 및 $4.5{\mu}g/mL$이었다. 에틸아세테이트 분획의 자유라디칼 소거 활성 및 총 항산화능이 가장 크게 나타났다. 또한 $^1O_2$로 유도된 적혈구 광용혈에 대한 세포 보호 효과(${\tau}_{50}$)는 금전초 50% 에탄올 추출물, 에틸아세테이트 분획 및 아글리콘 분획 $5{\mu}g/mL$에서 각각 26.9, 57.5 및 103.9 min을 나타냈다. 특히 아글리콘 분획물의 ${\tau}_{50}$은 (+)-${\alpha}$-tocopherol (37.7 min)보다 훨씬 큰 세포 보호 효과를 나타내었다. UVB로 유도된 세포 손상에서 에틸아세테이트 분획물은 최대 90.1%까지 세포 생존율을 증가시켰다. 과산화수소로 유도된 세포 손상에서도 에틸아세테이트 분획물은 $5-25{\mu}g/mL$에서 농도 의존적으로 세포 보호 효과를 나타내었다. 금전초 에틸아세테이트 분획물의 성분 분석을 위해 TLC, HPLC, UV-vis spectrum, LC-MS로 분석한 결과, quercetin, kaempferol 및 그 배당체가 주성분들로 확인되었다. 결론적으로 금전초가 자외선에 노출된 피부를 보호하는 항산화제로서 작용할 수 있고, 기능성 화장품 원료로 응용 가능성이 있음을 시사한다.

In the present study, we investigated the antioxidative properties, cellular protective effects and component analyses of 50% ethanol extract, ethyl acetate fraction and aglycone fraction obtained from Lysimachia christinae Hance (L. christinae Hance). In the evaluation of antioxidative properties, the free radical scavenging activities ($FSC_{50}$) of 50% ethanol extract, ethyl acetate fraction and aglycone fraction were 146.8, 22.2 and $27.2{\mu}g/mL$, respectively and total antioxidant capacities ($OSC_{50}$) were 29.3, 2.9 and $4.5{\mu}g/mL$, respectively. The ethyl acetate fraction showed the highest free radical scavenging activity and total antioxidant capacity. Also, the cellular protective effects (${\tau}_{50}$) of 50% ethanol extract, ethyl acetate fraction and aglycone fraction on $^1O_2$ induced photohemolysis of human erythrocytes were 26.9, 57.5 and 103.9 min at $5{\mu}g/mL$, respectively. In particular, ${\tau}_{50}$ of the aglycone fraction exhibited a higher cellular protective effect than that of (+)-${\alpha}$-tocopherol (37.7 min). The cell viability of the ethyl acetate fraction on the UVB-induced cell damage increased up to 90.1%. In addition, the ethyl acetate fraction ($5-25{\mu}g/mL$) showed cellular protective effects on the $H_2O_2-induced$ cell damages in a dose-dependent manner. TLC, HPLC, UV-vis spectroscopy and LC-MS were used to analyse components of the ethyl acetate fraction and the main components were quercetin, kaempferol and their glycosides. In conclusion, L. christinae Hance extract/fraction can function as antioxidants to protect the skin exposed to UV radiation and may also be used as a novel functional cosmetic material, for example, an antioxidant against skin photoaging.

키워드

참고문헌

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