Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Ecotoxic Evaluations of BDE-47 and BDE-209 using Rotifer (Brachionus plicatilis)

해산로티퍼 (Brachionus plicatilis)를 이용한 브롬화난연제 (BDE-47, BDE-209)의 생태독성평가

  • Choi, Hoon (Marine Ecological Risk Assessment Center, West Sea Fisheries Research Institute, National Institute of Fisheries Science (NIFS)) ;
  • Lee, Ju-Wook (Marine Ecological Risk Assessment Center, West Sea Fisheries Research Institute, National Institute of Fisheries Science (NIFS)) ;
  • Park, Yun-Ho (Marine Ecological Risk Assessment Center, West Sea Fisheries Research Institute, National Institute of Fisheries Science (NIFS)) ;
  • Lee, In-Seok (Marine Environment Research Division, National Institute of Fisheries Science (NIFS)) ;
  • Heo, Seung (Marine Ecological Risk Assessment Center, West Sea Fisheries Research Institute, National Institute of Fisheries Science (NIFS)) ;
  • Hwang, Un-Ki (Marine Ecological Risk Assessment Center, West Sea Fisheries Research Institute, National Institute of Fisheries Science (NIFS))
  • 최훈 (국립수산과학원 서해수산연구소 해양생태위해평가센터) ;
  • 이주욱 (국립수산과학원 서해수산연구소 해양생태위해평가센터) ;
  • 박윤호 (국립수산과학원 서해수산연구소 해양생태위해평가센터) ;
  • 이인석 (국립수산과학원 어장환경과) ;
  • 허승 (국립수산과학원 서해수산연구소 해양생태위해평가센터) ;
  • 황운기 (국립수산과학원 서해수산연구소 해양생태위해평가센터)
  • Received : 2018.02.22
  • Accepted : 2018.03.02
  • Published : 2018.03.31
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Abstract

The toxic assessment of the PBDEs (BDE-47, BDE-209) has been comprehensively investigated by using the rates of survival and population growth in the marine rotifer, Brachionus plicatilis. Chiefly, the survival rate was determined after a measurement of 24 hours of exposure to the BDE-47 (2,2'4,4'-Tetrabromodiphenyl ether) and the BDE-209 (2,2',4,4'-Decabromodiphenyl ether) was performed. The BDE-47 reduced survival rate in dose-dependent manner and a significant reduction were noted to have occurred at a concentration of greater than $3.9mg\;L^{-1}$, but the BDE-209 had no effect which was subsequently observed in this study. The population growth rate (r) was determined after 72 hours of exposure to toxicants in the study. It was observed that the r value in the controls (absence PBDEs) was greater than 0.5, and that it decreased as the dose-dependent manner as recorded. The survival rate when exposed to BDE-47 and BDE-209, $EC_{50}$ value was $13mg\;L^{-1}$ and $>1,000mg\;L^{-1}$, and population growth rate was $3.67mg\;L^{-1}$ and $862.75mg\;L^{-1}$, respectively. Therefore, the BDE-47 is considered to be 76-235 times more harmful than the BDE-209 as noted. In this study, the ecotoxicological bioassay using a noted survival rate and population growth rate of B. plicatilis can be used as a baseline data for the continued establishment of the environmental quality standard of the incidences of the BDE-47 and BDE-209 in a marine environment.

해산로티퍼, B. plicatilis의 생존율과 개체군 성장률을 이용하여 PBDEs (BDE-47, BDE-209)의 생태독성평가를 수행하였다. 생존율의 경우 BDE-47은 $3.9mg\;L^{-1}$ 이상의 농도에서 유의하게 감소하여 농도의존성을 나타냈으나, BDE-209의 경우는 큰 변화를 나타내지 않았다. 개체군 성장률의 경우는 BDE-47과 BDE-209에서 각각 농도의존적으로 감소하였다. 본 시험결과 $EC_{50}$값은 생존율의 경우 각각 $13mg\;L^{-1}$과 $1,000mg\;L^{-1}$이었고 개체군 성장률은 각각 $3.67mg\;L^{-1}$과 $862.75mg\;L^{-1}$로 나타나 BDE-47이 BDE-209에 비해 76~235배 더 독성이 강한 것으로 나타났다. 또한 BDE-47과 BDE-209에 대한 생존율의 LOEC값은 각각 $3.90mg\;L^{-1}$, $>1,000mg\;L^{-1}$으로 나타났으며, 개체군 성장률의 LOEC값은 각각 $1.95mg\;L^{-1}$, $125mg\;L^{-1}$로 나타났다. 본 연구를 통하여 도출된 LOEC값은 연안환경에서의 BDE-47과 BDE-209농도가 이를 초과하는 경우 B. plicatilis와 같은 동물성 플랑크톤에 악영향을 미칠 것으로 판단된다. 또한 NOEC와 $EC_{50}$값은 BDE-47 및 BDE-209와 같은 독성 물질의 환경 기준을 수립하기 위한 귀중한 자료로 활용될 수 있을 것이다.

Keywords

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