Journal of Plant Biotechnology

  • 제45권4호
  • /
  • Pages.364-368
  • /
  • 2018
  • /
  • 1229-2818(pISSN)
  • /
  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
권호 표지
DOI QR코드

DOI QR Code

고구마의 IbMYB1 유전자를 이용한 안토시아닌 고함유 형질전환 감자의 개발

Development of transgenic potato with improved anthocyanin contents using sweet potato IbMYB1 gene

  • 김윤희 (국립경상대학교 사범대학 생물교육과(농업생명과학연구원)) ;
  • 한은희 (경남농업기술원, 약용자원연구소) ;
  • 곽상수 (한국생명공학연구원, 식물시스템공학연구센터) ;
  • 이신우 (국립경남과학기술대학교 생명과학대학 농학.한약자원학부)
  • Kim, Yun-Hee (Department of Biology Education, College of Education, IALS, Gyeongsang National University) ;
  • Han, Eun-Hee (Medicinal Recourses Research Institute, Gyeongnam Agricultural Research & Extension Services) ;
  • Kwak, Sang-Soo (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Lee, Shin-Woo (Department of Agronomy & Medicinal Plant Resources, Gyeongnam National University of Science & Technology)
  • 투고 : 2018.10.22
  • 심사 : 2018.11.06
  • 발행 : 2018.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

R2R3 유형의 단백질인 IbMYB1 전사인자는 고구마의 뿌리에서 안토시아닌 생합성 과정을 조절하는 중요한 단백질이다. 선행연구에서 IbMYB1 유전자의 발현 증가를 통한 안토시아닌의 합성 증가가 담배, 애기장대 및 고구마의 저장뿌리에서 증명된 바 있다. 본 연구에서는 괴경(저장줄기) 특이적 PATATIN 프로모터와 산화스트레스 유도성 SWPA2 프로모터의 조절하에서 안토시아닌을 고함유하는 IbMYB1 유전자 과발현 형질전환 감자를 개발하여 그 특성을 분석하였다. PAT-IbMYB1 형질전환 식물체들은 대조구 식물체 및 SWPA2-IbMYB1 식물체 보다 높은 안토시아닌 함량을 괴경에서 나타내었다. 본 연구의 결과로서, IbMYB1의 과발현은 형질전환 기술을 이용한 특정 조직 특이적 안토시아닌 생산에 매우 좋은 개발 기술이 될 것으로 생각되는 바이다.

The R2R3-type protein IbMYB1 transcription factor is a key regulator for anthocyanin biosynthesis in the storage roots of sweet potatoes. It was previously demonstrated that the IbMYB1 expression stimulates anthocyanin pigmentation in tobacco leaves, arabidopsis and storage roots of sweet potatoes. In this study, we generated the transgenic potato plants that express the IbMYB1 genes, which accumulated high levels of anthocyanins under the control of either the tuber-specific patatin (PAT) promoter or oxidative stress-inducible peroxidase anionic 2 (SWPA2) promoter. The PAT-MYB1 transgenic lines exhibited higher anthocyanin levels in the tuber than the empty vector control (EV) or SWPA2-MYB1 plants. When combined, our results indicated that overexpression of the IbMYB1 is a highly promising strategy for the generation of transgenic plants with enhanced tissue specific anthocyanin production.

키워드

참고문헌

  1. Allan AC, Hellens RP, Laing WA (2008) MYB transcription factors that colour our fruit. Trends Plant Sci 13:99-102 https://doi.org/10.1016/j.tplants.2007.11.012
  2. Borevitz JO, Xia Y, Blount J, Dixon RA, Lamb C (2000) Activation tagging identifies a conserved MYB regulator of phenylpropanoid biosynthesis. Plant Cell 12:2383-2393 https://doi.org/10.1105/tpc.12.12.2383
  3. Cone KC, Cocciolone SM, Burr FA, Burr B (1993) Maize anthocyanin regulatory gene pl is a duplicate of c1 that functions in the plant. Plant Cell 5:1795-1805 https://doi.org/10.1105/tpc.5.12.1795
  4. Chen PN, Chu SC, Chiou HL, Chiang CL, Yang SF, Hsieh YS (2005) Cyanidin 3-glucoside and peonidin 3-glucoside inhibit tumor cell growth and induce apoptosis in vitro and suppress tumor growth in vivo. Nutr Cancer 53:232-243 https://doi.org/10.1207/s15327914nc5302_12
  5. Cho KS, Han EH, Kwak SS, Cho JH, Im JS, Hong SY, Sohn HB, Kim YH, Lee SW (2016) Expressing the sweet potato orange gene in transgenic potato improves drought tolerance and marketable tuber production. Compt Rend Biol 339:207-213 https://doi.org/10.1016/j.crvi.2016.04.010
  6. Dubos C, Le Gourrierec J, Baudry A, Huep G, Lanet E, Debeaujion I, Routaboul JM, Alboresi A, Weisshaar B, Lepiniec L (2008) MYBL2 is a new regulator of flavonoid biosynthesis in Arabidopsis thaliana. Plant J 55:940-953 https://doi.org/10.1111/j.1365-313X.2008.03564.x
  7. Espley RV, Bovy A, Bava C, Jaeger SR, Tomes S, Norling C, Crawford J, Rowan D, McGhie TK, Brendolise C, Putterill J, Schouten HJ, Hellens RP, Allan AC (2013) Analysis of genetically modified red-fleshed apples reveals effects on growth and consumer attributes. Plant Biotechnol J 11:408-419 https://doi.org/10.1111/pbi.12017
  8. Espley RV, Hellens RP, Putterill J, Stevenson DE, Kutty-Amma S, Allan AC (2007) Red colouration in apple fruit is due to the activity of the MYB transcription factor, MdMYB10. Plant J 49:414-427 https://doi.org/10.1111/j.1365-313X.2006.02964.x
  9. Goo YM, Han EH, Jeong JC, Kwak SS, Yu J, Kim YH, Ahn MJ, Lee SW (2015) Overexpression of the sweet potato IbOr gene results in the increased accumulation of carotenoid and confers tolerance to environmental stresses in transgenic potato. Compt Rend Biol 338:12-20 https://doi.org/10.1016/j.crvi.2014.10.006
  10. Holton and Comish (1995) Genetics and biochemistry of anthocyanin biosynthesis. Plant Cell 7:1071-1083 https://doi.org/10.1105/tpc.7.7.1071
  11. Kim CY, Ahn YO, Kim SH, Kim YH, Lee HS, Catanach AS, Jacobs JME, Conner AJ, Kwak SS (2010) The sweet potato IbMYB1 gene as a potential visible marker for sweet potato intragenic vector system. Physiol Plant 139:229-240
  12. Kobayashi S, Ishimaru M, Hiraoka K, Honda C (2002) Myb-related genes of the Kyoho grape (Vitis labruscana) regulate anthocyanin biosynthesis. Planta 215:924-933 https://doi.org/10.1007/s00425-002-0830-5
  13. Mano H, Ogasawara F, Sato K, Higo H, Minobe Y (2007) Isolation of a regulatory gene of anthocyanin biosynthesis in tuberous roots of purple-fleshed sweet potato. Plant Physiol 143:1252-1268 https://doi.org/10.1104/pp.106.094425
  14. Murashige T and Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  15. Park SC, Kim YH, Kim SH, Jeong YJ, Kim CY, Lee JS, Bae JY, Ahn MJ, Jeong JC, Lee HS and Kwak SS (2015) Overexpression of the IbMYB1 gene in an orange-fleshed sweet potato cultivar produces a dual-pigmented transgenic sweet potato with improved antioxidant activity. Physiol Plant 153:525-537 https://doi.org/10.1111/ppl.12281
  16. Tanaka Y, Sasaki N, Ohmiya A (2008) Biosynthesis of plant pigments: anthocyanins, betalains and carotenoids. Plant J 54:733-749 https://doi.org/10.1111/j.1365-313X.2008.03447.x
  17. Teow CC, Truong V-D, McFeeters RF, Thompson RL, Pecota KV, Yencho GC (2007) Antioxidant activities, phenolic and ${\beta}$ -carotene contents of sweet potato genotypes with varying flesh colours. Food Chem 103:829-838 https://doi.org/10.1016/j.foodchem.2006.09.033