한국수산과학회지 (Korean Journal of Fisheries and Aquatic Sciences)

  • 제51권6호
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  • Pages.688-696
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  • 2018
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  • 0374-8111(pISSN)
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  • 2287-8815(eISSN)
한국수산과학회 (The Korean Society of Fisheries and Aquatic Science)
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반순환여과시스템에서 오존 유래 잔류산화물 농도에 따른 넙치(Paralichthys olivaceus)의 성장과 생리학적 변화

Growth Performance and Physiological Changes of Olive Flounder Paralichthys olivaceus by Concentration of Ozone Produced Oxidants in Semi-RAS

  • 정상명 (강원도립대학교 해양양식식품과) ;
  • 박우근 (강원도립대학교 해양양식식품과) ;
  • 박정환 (부경대학교 해양바이오신소재학과) ;
  • 김재원 (강원도립대학교 해양양식식품과) ;
  • 김병기 (강원도립대학교 해양양식식품과)
  • Jung, Sangmyung (Department of Aquaculture and Seafood, Gangwon State University) ;
  • Park, Woogeun (Department of Aquaculture and Seafood, Gangwon State University) ;
  • Park, Jeonghwan (Department of Marine Bio-Materials and Aquaculture, Pukyong National University) ;
  • Kim, Jaewon (Department of Aquaculture and Seafood, Gangwon State University) ;
  • Kim, Pyong-kih (Department of Aquaculture and Seafood, Gangwon State University)
  • 투고 : 2018.11.21
  • 심사 : 2018.12.14
  • 발행 : 2018.12.31
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초록

This study investigated the effects of ozone-produced oxidants (OPO) on the growth, hematology, and histology of olive flounder Paralichthys olivaceus (average weight 500 g), raised in an ozonated semi-recirculating aquaculture system. The system was ozonated to maintained OPO concentrations of 0.004 (Control), 0.014 (OPO15), and 0.025 (OPO25) mg $Cl_2/L$ in culture tanksfor 26 days. The specific growth rate, feed conversion ratio, and survival rate did not significantly differ among the groups (P>0.05), while the daily feeding rate decreased OPO-dose-dependently (P<0.05). OPO appeared to affect the gill, hepatopancreas, and kidney tissues of fish from ozonated tanks. Hematologically, OPO affected some blood indices. The levels of chloride, glucose, glutamic oxaloacetic transaminase, and glutamic pyruvic transaminase were significantly increased in the ozonated groups, while the total cholesterol and cortisol decreased dose-dependently. These results imply that long-term exposure of olive flounder to an OPO concentration ${\geq}0.014mg\;Cl_2/L$ might result in damage to the gill, hepatopancreas, and kidney tissues and cause physiological stress, albeit with no apparent short-term effects on growth or survival.

키워드

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Fig. 1. Experimental semi-RAS system.

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Fig. 2. Histological changes of the gill of olive flounder Paralichthys olivaceus depending on the ozone injection rates. A: Control. Showing the gill lamellae (Gl). B: OPO15 group. H-E section showing the clubbed type of gill lamellae. C: OPO25 group. Note the hypertrophy and the epithelial cell lifting of gill lamellae. OPO, Ozone produced oxidants.

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Fig. 3. Histological changes of the liver of olive flounder Paralichthys olivaceus depending on the ozone injection rates. A: Control. Note the hepatic cell (Hc), pancreas (P) and bile duct (Bd). B: OPO15 group. a little degeneration of hepatic cells and zymogen granules. C: OPO25 group. Note the degeneration of hepatic cells and the abnormal type of pancreas. OPO, Ozone produced oxidants.

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Fig. 4. Histological changes of the kidney of olive flounder Paralichthys olivaceus depending on the ozone injection rates. A: Control. Note normal glomerulus (Gl) and renal tubule (Rt). B: OPO15 group. Note a little activated melano-macrophagocytes and the hypertrophied epithelia of renal tubules. D: OPO25 group. Section showing the deformation and degeneration of glomerulus and renal tubule. Fig. 5 Blood analysis of olive flounder Paralichthys olivaceus reared at different OPO concentration. OPO, Ozone produced oxidants.

KSSHBC_2018_v51n6_688_f0005.png 이미지

Fig. 5. Blood analysis of olive founder Paralichthys olivaceus reared at different OPO concentration. OPO, Ozone produced oxidants;GOT, Glutamic oxaloacetic transaminase; GPT, Glutamic pyruvic transaminase.

Table 1. Water environment for the experiment

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Table 2. Growth performance of olive founder Paralichthys olivaceus reared with 3 different OPO concentrations for 26 days

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Table 3. The OPO concentration-dependent correlation of olive founder Paralichthys olivaceus blood indicators

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