Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Quantitative Analysis of Bergenin in Astilbe rubra and Growth Inhibition Effect of Ethanol Extract on Hela Cells

노루오줌으로부터 Bergenin의 함량 분석 및 에탄올 추출물의 자궁경부암세포 성장 억제 효과

  • Kim, A Hyeon (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine) ;
  • Nho, Jong Hyun (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine) ;
  • Woo, Kyeong Wan (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine) ;
  • Gang, Ju Eun (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine) ;
  • Sim, Mi Ok (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine) ;
  • Kim, Min Suk (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine) ;
  • Ham, Seong Ho (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine) ;
  • Cho, Hyun Woo (Division of Traditional Korean Medicine Resource, National Development Institute of Korea Medicine)
  • 김아현 (한약진흥재단 한약자원본부) ;
  • 노종현 (한약진흥재단 한약자원본부) ;
  • 우경완 (한약진흥재단 한약자원본부) ;
  • 강주은 (한약진흥재단 한약자원본부) ;
  • 심미옥 (한약진흥재단 한약자원본부) ;
  • 김민석 (한약진흥재단 한약자원본부) ;
  • 함성호 (한약진흥재단 한약자원본부) ;
  • 조현우 (한약진흥재단 한약자원본부)
  • Received : 2017.09.11
  • Accepted : 2018.01.18
  • Published : 2018.03.31
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Abstract

Astilbe rubra (AR) is a perennial, belongs to the Saxifragaceae family, it contains tannin and triterpene. AR has been used in republic korea to improve toxication, fever, pain and convulsion. Recently, number of natural products have been analyzed for potential pharmacological activities including anti-cancer, anti-obesity and anti-diabetic medication. Consequently, we investigated the growth inhibition effect of Astilbe rubra water extract (WAC), ethanol extract (EAC) and bergenin on Hela cell (human adenocarcinoma cell). From whole plant of A. rubra, bergenin was isolated by column chromatography and its structures were identified by $^1H$, $^{13}C$ NMR and IT TOF-ESI MS. High extraction efficiency of bergenin was shown at 0.95% under 60 min reflux extraction with 50% MeOH. The MTS assay showed that EAC (ethanol extract) treatment increased cell death in a dose-dependent manner. Moreover, EAC treatment on Hela cell increased apoptotic cell death and caspase-3 activity. Results suggest that EAC has growth inhibition effect on Hela cells, but not WAC and bergenin. $500{\mu}g/mL$ EAC treatment inhibited Hela cell at $60.2{\pm}1.5%$.

Keywords

References

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