Journal of Korean Dental Science

  • 제11권2호
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  • Pages.43-56
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  • 2018
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  • 2005-4742(pISSN)
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  • 2713-7651(eISSN)
대한치의학회 (Korean Academy of Dental Science)
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Bone Regeneration Using Block-type Deproteinized Porcine Bone Mineral with Collagen Membrane Using 3,4-Dihydroxyphenylalanine as Bone Adhesive

  • Kang, Joo Hyun (Department of Periodontology, Research Institute of Periodontal Regeneration, Yonsei University College of Dentistry) ;
  • Pae, Hyoung-Chul (Department of Periodontology, Research Institute of Periodontal Regeneration, Yonsei University College of Dentistry) ;
  • Cha, Jae-Kook (Department of Periodontology, Research Institute of Periodontal Regeneration, Yonsei University College of Dentistry) ;
  • Lee, Jung-Seok (Department of Periodontology, Research Institute of Periodontal Regeneration, Yonsei University College of Dentistry) ;
  • Paik, Jeong-Won (Department of Periodontology, Research Institute of Periodontal Regeneration, Yonsei University College of Dentistry) ;
  • Jung, Ui-Won (Department of Periodontology, Research Institute of Periodontal Regeneration, Yonsei University College of Dentistry) ;
  • Choi, Seong-Ho (Department of Periodontology, Research Institute of Periodontal Regeneration, Yonsei University College of Dentistry)
  • 투고 : 2018.11.30
  • 심사 : 2018.12.27
  • 발행 : 2018.12.30
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초록

Purpose: The purpose of this study was to assess the adhesiveness and cytotoxicity of 3, 4-dihydroxyphenylalanine (DOPA), and to evaluate the role of collagen membrane with DOPA in the guided bone regeneration. Materials and Methods: Peel resistance and cell cytotoxicity test were performed. Four defect types in nine rabbit calvaria were randomly allocated: i) control, ii) membrane, iii) deproteinized porcine bone mineral (DPBM) covered by membrane with DOPA, and iv) DPBM covered by membrane with cyanoacrylate. Animals were sacrificed at 2 (n=4) and 8 weeks (n=5) for microcomputed tomography and histomorphometric analysis. DOPA showed low peel resistance but high cell viability. Result: Cyanoacrylate and DOPA groups showed significantly higher mineralized tissue volume (MTV) compared to control and membrane groups at 2 weeks (P<0.05). At 8 weeks, DOPA group showed the highest MTV. Significantly higher new bone area was found in DOPA group at 8 weeks (P<0.05). Bone formation increased from 2 to 8 weeks in DOPA group (P<0.05). Conclusion: DOPA showed high cell viability and in vivo study revealed predictable performance in bone regeneration.

키워드

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Fig. 1. Clinical photographs of the experimental sites. (A) Four circumferential defects with 8 mm diameter in rabbit calvarium. (B) Random assignment of defects, from top left in clockwise; membrane group, cyanoacrylate group, 3,4-dihydroxyphenylalanine (DOPA) group, and control group.

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Fig. 2. Peel resistance test. Cyanoacrylate, when applied to collagen membrane, shows higher resistance to peeling load compared to 3,4-dihydroxyphenylalanine (DOPA).

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Fig. 3. Cell cytotoxicity test. Cyanoacrylate (T1) shows the lowest cell viability whereas 3,4-dihydroxyphenylalanine (DOPA) (T2) shows the highest value. BC: blank control group, NC: negative control group, PC: positive control group, C: control group, T1: cyanoacrylate group, T2: DOPA group.

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Fig. 4. Three-dimensionally reconstructed micro-computed tomography images of defects at 2 weeks. (A) Control group. (B) Membrane group. (C) Cyanoacrylate group. (D) 3,4-Dihydroxyphenylalanine (DOPA) group.

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Fig. 5. Three-dimensionally reconstructed micro-computed tomography images of defects at 8 weeks. (A) Control group. (B) Membrane group. (C) Cyanoacrylate group. (D) 3,4-Dihydroxyphenylalanine (DOPA) group.

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Fig. 6. Histologic view after 2 weeks of healing showing (A) control group, defect space is mostly empty; (B) membrane group, collagen membrane is intact with good adaptation; (C) cyanoacrylate group, space is well-maintained by bone graft materials with overlying intact collagen membrane; (D) 3,4-dihydroxyphenylalanine (DOPA) group, collagen membrane is overlaid on defect with supporting bone graft particles. Masson trichrome staining. Scale bars=1 mm.

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Fig. 7. Histologic view after 8 weeks of healing showing (A) control group, almost full closure of defect by bony bridge is observed; (B) membrane group, only a small amount of bony islands is formed with partially resorbed collagen membrane; (C) cyanoacrylate group, newly formed bone from the margin of defect is present; (D) 3,4-dihydroxyphenylalanine (DOPA) group, more mature bone is present inside the bone graft scaffold compared to 2 weeks. Masson trichrome staining. Scale bars=1 mm.

Table 1. Values of MTV measured by micro-computed tomography grey value

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Table 2. Histomorphometric analysis

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