한국축산식품학회지 (Food Science of Animal Resources)

  • 제38권6호
  • /
  • Pages.1168-1178
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  • 2018
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  • 2636-0772(pISSN)
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  • 2636-0780(eISSN)
한국축산식품학회 (Korean Society for Food Science of Animal Resources)
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Anti-proliferative Effect of a Novel Anti-oxidative Peptide in Hanwoo Beef on Human Colorectal Carcinoma Cells

  • Kim, Hye-Jin (Department of Animal Life Science, College of Animal Life Science) ;
  • Yang, Se-Ran (Department of Thoracic and Cardiovascular Surgery, School of Medicine, Kangwon National University) ;
  • Jang, Aera (Department of Animal Life Science, College of Animal Life Science)
  • 투고 : 2018.07.31
  • 심사 : 2018.10.18
  • 발행 : 2018.12.31
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초록

The present study aimed to characterise anti-oxidant peptides from water-soluble protein extracts of Hanwoo beef and evaluate their anti-proliferative effect on human colorectal carcinoma cells (HCT116). Antioxidant peptides were purified from the low-molecular-weight fraction (<3 kDa) of Hanwoo beef extract. Antioxidant activity of peptide fractions was determined using the oxygen radical absorbance capacity (ORAC) assay. Purified peptide (P3) displayed higher ORAC activity than the low-molecular-weight fraction ($202.66{\mu}M\;TE/g$ vs $167.38{\mu}M\;TE/g$ of dry matter, respectively) (p<0.05). The peptide sequence of P3 was Cys-Cys-Cys-Cys-Ser-Val-Gln-Lys (888.30 Da). The novel peptide P3, at $250{\mu}g/mL$, also significantly inhibited HCT116 cell proliferation up to 25.24% through phosphorylation of ERK, JNK, and p38 kinase (p<0.05). Hence, antioxidant peptide P3 from Hanwoo beef extract can be used as an antioxidative and anticancer agent in the functional food industry.

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과제정보

연구 과제 주관 기관 : IITP (Institute for Information & communications Technology Promotion)

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