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Analysis of Phylogenetic Relationship of 30 Cultivars of Korean Mulberry (Rosales: Moraceae) in Korea

  • Kwon, O-Chul (Sericultural & Apicultural Materials Division, National Institute of Agricultural Science, Rural Development Administration) ;
  • Kim, Hyun-Bok (Sericultural & Apicultural Materials Division, National Institute of Agricultural Science, Rural Development Administration) ;
  • Sung, Gyoo-Byung (Sericultural & Apicultural Materials Division, National Institute of Agricultural Science, Rural Development Administration) ;
  • Kim, Yong-Soon (Sericultural & Apicultural Materials Division, National Institute of Agricultural Science, Rural Development Administration) ;
  • Ju, Wan-Taek (Sericultural & Apicultural Materials Division, National Institute of Agricultural Science, Rural Development Administration)
  • 투고 : 2018.11.26
  • 심사 : 2018.12.13
  • 발행 : 2018.12.31

초록

This study was carried out to understand phylogenetic relationships of the 30 mulberry cultivars converved in Korea based on the ITS rDNA region, and they were compared to 40 reference sequences from GenBank. The size and the G+C content of the ITS rDNA gene regions from the 30 Korean mulberry cultivars and 40 reference sequences varied from 612-630 bp and 58.19-61.62%, respectively. Based on the results of the comparative phylogenetic analysis of the ITS rDNA regions of the 30 Korean mulberry cultivars and 40 reference sequences, they were divided into three groups (Group 1, 2, and 3) and two subgroups (Group 1A and 1B within Group 1). The sequence lengths of the Korean mulberry cultivar numbers 1-26 and 27-30 were 615 bp and 616 bp, respectively. At 205 bp location of ITS1 rDNA region, the cultivar numbers 1-26 contain the nucleotide thymine but the cultivar numbers 27-30 contain the nucleotide adenine. In addition, the insertion of the nucleotide adenine at 206 bp location was found only in the four Korean mulberry cultivars (numbers 27-30). Based on these sequence information and phylogenetic result, the 30 Korean mulberry cultivars were identified as M. alba and M. australis. This study will contribute to the construction of genetic database constructions and accurate variety identifications for unidentified mulberry varieties in Korea.

키워드

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Fig. 1. Phylogenetic relationships based on ITS rDNA region sequences among 30 Korean mulberry cultivars and 40 reference sequences. The tree was obtained using the neighbor–joining method. Numbers at the branch nodes represent bootstrap values obtained from 1,000 replications. Two strains of Artocarpus heterophyllus and Broussonetia papyrifera were used as the outgroups.

Table 1. Sequence information for ITS region of Korean mulberry cultivars used in this study

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Table 2. Information for ITS rDNA region of reference sequence (NCBI databases)

E1IEAM_2018_v37n2_82_t0002.png 이미지

Table 3. Sequence difference of ITS 1 region between the two Korean mulberry cultivar groups

E1IEAM_2018_v37n2_82_t0003.png 이미지

참고문헌

  1. Alvarez I, Wendel JF (2003) Ribosomal ITS sequences and plant phylogenetic inference. Mol Phylogenet Evol 29, 417-434. https://doi.org/10.1016/S1055-7903(03)00208-2
  2. Awasthi AK, Nagaraja GM, Naik GV, Kanginakudru S, Thangavelu K, Nagaraju J (2004) Genetic diversity and relationships in mulberry (genus Morus) as revealed by RAPD and ISSR marker assays. BMC Genet 5, 1.
  3. Brandis D (1874) The forest flora of northwest and central India. In Indian trees. pp. 407-410, William H. Allen & Co., London.
  4. Bureau E (1873) Prodromus systematis naturalis regni vegetabilis. DeCandolle AP (eds.), pp. 211-288, Tuetteland Wurtz, Paris.
  5. Cao H, But PP, Shaw PC (1998) Methodological studies on genomic DNA extraction and purification from plant drug materials. J Chin Pharm Sci 7, 130-137.
  6. Chen RF, Yu MD, Liu XQ, Chen LQ (2010) Analysis on the internal transcribed spacers (ITS) sequences and phylogenetics of mulberry (Morus). Sci Agri Sinica 43, 1771-1781.
  7. Felsenstein J (1985) Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39, 783-791. https://doi.org/10.1111/j.1558-5646.1985.tb00420.x
  8. He N, Zhang C, Qi X, Zhao S, Tao Y, Yang G, et al. (2013). Draft genome sequence of the mulberry tree Morus notabilis. Nat Commun 4, 1-9.
  9. Hotta T (1954) Fundamentals of Morus plants classification. Kinugasa Sanpo 390, 13-21.
  10. Jeong JH, Lee NK, Cho SH, Jeong YS (2014). Enhancement of 1-deoxynojirimycin content and ${\alpha}$-glucosidase inhibitory activity in mulberry leaf using various fermenting microorganisms isolated from Korean traditional fermented food. Biotechnol Bioprocess Eng 19, 1114-1118. https://doi.org/10.1007/s12257-014-0277-0
  11. Kalpana D, Choi SH, Choi TK, Senthil K, Lee YS (2012) Assessment of genetic diversity among varieties of mulberry using RAPD and ISSR fingerprinting. Sci Hortic 134, 79-87. https://doi.org/10.1016/j.scienta.2011.11.002
  12. Kapche GD, Fozing CD, Donfack JH, Fotso GW, Amadou D, Tchana AN, et al. (2009) Prenylated arylbenzofuran derivatives from Morus mesozygia with antioxidant activity. Phytochemistry 70, 216-221. https://doi.org/10.1016/j.phytochem.2008.12.014
  13. Koidzumi G (1917) Taxonomical discussion on Morus plants. Bull Imp Sericult Exp Stat 3, 1-62.
  14. Kumar S, Stecher G, Tamura K (2016) MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol Biol Evolution 33, 1870-1874. https://doi.org/10.1093/molbev/msw054
  15. Leroy JF (1949) Les Muriers sauvages et cultives. La sericiculture sous les tropiques Rev. J Agric Tradit Bot Appl 29, 481-496.
  16. Li DZ, Gao LM, Li HT, Wang H, Ge XJ, Liu JQ, et al. (2011) Comparative analysis of a large dataset indicates that internal transcribed spacer (ITS) should be incorporated into the core barcode for seed plants. Proc Natl Acad Sci USA 108, 19641-19646. https://doi.org/10.1073/pnas.1104551108
  17. Linnaeus C (1753) Morus (In Species Plantarum Vol. 2), pp. 968, Impensis Laurentii Salvii, Stockholm.
  18. Nepal MP (2008) Systematics and reproductive biology of the genus Morus L.(Moraceae). Ph.D. Thesis, Kansas State University, Manhattan.
  19. Nepal MP, Ferguson CJ (2012). Phylogenetics of Morus (Moraceae) Inferred from ITS and trnL-trnF Sequence Data. Syst Bot 37, 442-450.
  20. Pawlowska AM, Oleszek W, Braca A (2008) Quali-quantitative analyses of flavonoids of Morus nigra L. and Morus alba L.(Moraceae) fruits. J Agric Food Chem 56, 3377-3380. https://doi.org/10.1021/jf703709r
  21. Rao DMR, Jhansilakshmi K, Saraswathi P, Rao AA, Ramesh S, Borpuzari M, et al. (2013) Scope of pre-breeding in mulberry crop improvement-A review. Sci Wkly 1, 1-18.
  22. Saitou N, Nei M (1987) The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406-425.
  23. Schneider CK (1917) Plantae Wilsonianae: an enumeration of the woody plants collected in western China for the Arnold arboretum of Harvard university during the years 1907, 1908, and 1910. Sargent CS (eds.), pp. 293-294, The University Press, Cambridge.
  24. Sharma A, Sharma R, Machii H (2000) Assessment of genetic diversity in a Morus germplasm collection using fluorescence-based AFLP markers. Theor Appl Genet 101, 1049-1055. https://doi.org/10.1007/s001220051579
  25. Vijayan K, Srivatsava PP, Nair CV, Awasthi AK, Tikader A, Sreenivasa B, et al. (2006) Molecular characterization and identification of markers associated with yield traits in mulberry using ISSR markers. Plant Breeding 125, 298-301. https://doi.org/10.1111/j.1439-0523.2006.01212.x
  26. Zeng Q, Chen H, Zhang C, Han M, Li T, Qi X, et al. (2015) Definition of Eight Mulberry Species in the Genus Morus by Internal Transcribed Spacer-Based Phylogeny. PLoS ONE 10, e013541.
  27. Zhang SD, Soltis DE, Yang Y, Li DZ, Yi TS (2011) Multi-gene analysis provides a well-supported phylogeny of Rosales. Mol Phylogenet Evol 60, 21-28. https://doi.org/10.1016/j.ympev.2011.04.008
  28. Zhao W, Huang Y, Jia S, Miao X, Pan Y (2005) Isolation and characterization of microsatellite loci from the mulberry, Morus L. Plant Sci 168, 519-525. https://doi.org/10.1016/j.plantsci.2004.09.020
  29. Zhao WG, Pan YL, Zhang ZF (2004) Phylogenetic relationship of genus Morus by ITS sequence data. Sci Seric 30, 11-14.
  30. Zheng ZP, Tan HY, Wang M (2012) Tyrosinase inhibition constituents from the roots of Morus australis. Fitoterapia 83, 1008-1013. https://doi.org/10.1016/j.fitote.2012.06.001
  31. Zhu B, Lou MM, Xie GL, Wang GF, Zhou Q, Wang F, et al. (2011). Enterobacter mori sp. nov., associated with bacterial wilt on Morus alba L. Int J Syst Evol Microbiol 61, 2769-2774. https://doi.org/10.1099/ijs.0.028613-0