Journal of Microbiology and Biotechnology

  • 제28권3호
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  • Pages.349-356
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  • 2018
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Immune Enhancement Effect of Asterias amurensis Fatty Acids through NF-κB and MAPK Pathways on RAW 264.7 Cells

  • Monmai, Chaiwat (Department of Marine Food Science and Technology, Gangneung-Wonju National University) ;
  • Go, Seok Hyeon (Department of Marine Food Science and Technology, Gangneung-Wonju National University) ;
  • Shin, Il-shik (Department of Marine Food Science and Technology, Gangneung-Wonju National University) ;
  • You, SangGuan (Department of Marine Food Science and Technology, Gangneung-Wonju National University) ;
  • Lee, Hyungjae (Department of Food Engineering, Dankook University) ;
  • Kang, SeokBeom (Citrus Research Station, National Institute of Horticultural & Herbal Science, RDA) ;
  • Park, Woo Jung (Department of Marine Food Science and Technology, Gangneung-Wonju National University)
  • 투고 : 2017.09.04
  • 심사 : 2017.11.29
  • 발행 : 2018.03.28
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초록

Asterias amurensis is a marine organism that causes damage to the fishing industry worldwide; however, it has been considered a promising source of functional components. The present study aimed to investigate the immune-enhancing effects of fatty acids from three organs of A. amurensis on murine macrophages (RAW 264.7 cells). A. amurensis fatty acids boosted production of immune-associated factors such as nitric oxide (NO) and prostaglandin E2 in RAW 264.7 cells. A. amurensis fatty acids also enhanced the expression of critical immune-associated genes, including iNOS, $TNF-{\alpha}$, $IL-1{\beta}$, and IL-6, as well as COX-2. Western blotting showed that A. amurensis fatty acids stimulated the $NF-{\kappa}B$ and MAPK pathways by phosphorylation of $NF-{\kappa}B$ p-65, p38, ERK1/2, and JNK. A. amurensis fatty acids from different tissues resulted in different levels of $NF-{\kappa}B$ and MAPK phosphorylation in RAW 264.7 cells. The results increase our understanding of how A. amurensis fatty acids boost immunity in a physiological system, as a potential functional material.

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