Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지
DOI QR코드

DOI QR Code

Content Analysis of Rutin in the Leaves of Boehmeria nivea Harvested in Different Regions of South Korea by HPLC-UV

  • Cho, Sunghun (Department of Integrative Plant Science, Chung-Ang University) ;
  • Quilantang, Norman G. (Department of Integrative Plant Science, Chung-Ang University) ;
  • Lee, Ju Sung (Department of Integrative Plant Science, Chung-Ang University) ;
  • Kim, Young-Mi (Yeong-Gwang Agricultural Technology Center) ;
  • Kim, Ho Bang (Life Sciences Research Institute, Biomedic Co., Ltd.) ;
  • Cho, Eun Ju (Department of Food Science and Nutrition, Pusan National University) ;
  • Jung, Yong-Su (Yeong-Gwang Agricultural Technology Center) ;
  • Lee, Sanghyun (Department of Integrative Plant Science, Chung-Ang University)
  • Received : 2017.09.05
  • Accepted : 2017.09.30
  • Published : 2018.03.30
Download PDF
⟨ Previous Next ⟩

Abstract

Phytochemical analysis of Boehmeria nivea (Bn) leaves by medium pressure liquid chromatography led to the isolation of a flavonoid glycoside identified by spectroscopic analysis as rutin. The amount of rutin in the leaves of Bn harvested from nine regions in South Korea (Bn 1-9) which were collected on the months of June, July, August, and September was determined by HPLC-UV analysis. A gradient elution program that utilizes a $Discovery^{(R)}$ C18 ($4.6{\times}250mm$, $5{\mu}m$) column and mobile phase composed of 1% acetic acid-water: acetonitrile (90:10 to 60:40 for min) was followed. The injection volume and flow rate were $10{\mu}l$ and 1 mL/ min, respectively. UV detection was set at 350 nm. Results show that Bn-8 harvested in September reported the highest content of rutin among the samples analyzed. This study provides a basis for the optimal harvest time of Bn which maximizes the yield of rutin.

Keywords

References

  1. Angelini, L. G.; Lazzeri, A.; Levita, G.; Fontanelli, D.; Bozzi, C. Ind. Crops Prod. 2000, 11, 145-161.
  2. Paiva Junior, C. Z.; de Carvalho, L. H.; Fonseca, V. M.; Monteiro, S. N.; d' Almeida, J. R. M. Polym. Test. 2004, 23, 131-135. https://doi.org/10.1016/S0142-9418(03)00071-0
  3. Nam, S.; Netravali, A. N. Fibers Polym. 2006, 7, 372-379. https://doi.org/10.1007/BF02875769
  4. Xu, Q. -M.; Liu, Y. -L.; Li, X. -R.; Li, X.; Yang, S. -L. Nat. Prod. Res. 2011, 25, 640-647. https://doi.org/10.1080/14786419.2010.488230
  5. Lin, C. -C.; Yen, M. -H.; Lo, T. -S.; Linb, J. -M. J. Ethnopharmacol. 1998, 60, 9-17. https://doi.org/10.1016/S0378-8741(97)00122-0
  6. Sancheti, S.; Sancheti, S.; Bafna, M.; Kim, H. -R.; You, Y. -H.; Seo, S. -Y. Braz. J. Pharm. 2011, 21, 146-154. https://doi.org/10.1590/S0102-695X2011005000021
  7. Sung, M. J.; Davaatseren, M.; Kim, S. H.; Kim, M. J.; Hwang, J. -T. Pharm. Biol. 2013, 51, 1131-1136. https://doi.org/10.3109/13880209.2013.781196
  8. Tan, Z.; Wang, C.; Yi, Y.; Wang, H.; Li, M.; Zhou, W.; Tan, S.; Li, F. Sep. Purif. Technol. 2014, 132, 396-400. https://doi.org/10.1016/j.seppur.2014.05.048
  9. Cho, S.; Lee, D. G.; Jung, Y. -S.; Kim, H. B.; Cho, E. J.; Lee, S. Nat. Prod. Sci. 2016, 22, 134-139. https://doi.org/10.20307/nps.2016.22.2.134
  10. Couch, J. F.; Naghski, J.; Krewson, C. F. J. Am. Chem. Soc. 1952, 74, 424-425. https://doi.org/10.1021/ja01122a042
  11. Ohsawa, R.; Tsutsumi, T. Euphytica 1995, 86, 183-189.
  12. Chua, L. S. J. Ethnopharmacol. 2013, 150, 805-817. https://doi.org/10.1016/j.jep.2013.10.036
  13. Kraujalis, P.; Venskutonis, P. R.; Ibanez, E.; Herrero, M. J. Supercrit. Fluids 2015, 104, 234-242.
  14. Erlund, I.; Kosonen, T.; Alfthan, G.; Maenpaa, J.; Perttunen, K.; Kenraali, J.; Parantainen, J.; Aro, A. Eur. J. Clin. Pharmacol. 2000, 56, 545-553.
  15. Hosseinzadeh, H.; Nassiri-Asl, M. J. Endocrinol. Invest. 2014, 37, 783-788. https://doi.org/10.1007/s40618-014-0096-3
  16. Kitabayashi, H.; Ujihara, A.; Hirose, T.; Minami, M. Breeding Sci. 1995, 45, 189-194.
  17. Gevrenova, R.; Kitanov, G.; Ilieva, D. Pharm. Biol. 2007, 45, 149-155.

Cited by

  1. Enhancing the antioxidative effects of foods containing rutin and α‐amino acids via the Maillard reaction: A model study focusing on rutin‐lysine system vol.44, pp.1, 2018, https://doi.org/10.1111/jfbc.13086