Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Diagnostic ex vivo assay of glucose in live cell using voltammetry

  • Ly, Suw Young (Biosensor Research Institute, Seoul National University of Science & Technology) ;
  • Leea, Chang Hyun (Major in ICT-integrated Environment, Pyeongtaek University)
  • Received : 2018.11.27
  • Accepted : 2018.12.21
  • Published : 2018.12.31
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Abstract

The hand held voltammetry systems searched diabetic assay using glucose sensor of fluorine nafion doped carbon nanotube electrode (FCNE). An inexpensive graphite carbon pencil was used as an Ag/AgCl reference and Pt counter electrode. Upon combining and using three electrode systems, optimum square wave (SW) stripping results were attained to 1.0-9.0 ug/L with 8 points. Statistic RSD precision was of 6.02 % with n=15 in 0.1 mg/L glucose. After a total of 200 second accumulation times, analytical detection limit of 0.8 ug/L was obtained. This developed technique was applied to urine samples from diabetic patients urine for fluid analysis, it was determined that the sensor can be used with a diagnostics in the ex vivo of live cells and non treated biological fluid.

Keywords

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Fig. 1. The Bioelectronics-2 second version, simple and small with three electrode systems for trace biological analysis.

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Fig. 2. (A): CV effects for glucose variation with 0, 20, 40, 60, 80, 100, 120, 140, and 160 mg/L spike using PE; Figure 1(B): Electrode comparison for FNCE and PE with SW accumulation time of 30 sec, 0, 20, 40, 60 and 80 mg/L glucose variations, anodic stripping in 0.1M NH4H2PO4 electrolyte solution; Figure 1 (C); 15th repetition using 0.1 mg/L addition with optimum condition

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Fig. 3. (A): Variations of the SW accumulation time range for 0.0, to 260 seconds; (B): SW accumulation potential ranges from 0.2 to 1.6 V, additions of 20 mg/L glucose in the 0.1 M NH4H2PO4 electrolyte. Other parameters were used for the following optimum conditions: pH 4.06, 0.1 V SW amplitude, 15 Hz SW frequency, 4 mV increment potential, -2.0 V final potential, and 120-seconds accumulation time

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Fig. 4. (A): Using optimum conditions, linear working ranges of 0.02, 0.04 0.06 0.08 0.10, 0.12 and 0.14 mg/L spikes; (B): FCNE working ranges of 1, 2, 3, 5, 6, 7 and 8 ug/L spike in a 0.1M NH4H2PO4 electrolyte solution with a pH of 3.06, an SW amplitude of 0.1 V, an SW frequency of 15 Hz, an increment potential of 4 mV, an accumulation potential of 0.5 V, and an accumulation time of 120 seconds; (C) Patient urine test, the first curve represents the blank and 0.05 mL urine and 0.01, 0,02, 0.03, 0.04 ml standard glucose spike with the SW parameter used for optimum conditions

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