Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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A Study on Antimicrobial Activity of Lysimachia clethroides Duby Root Extracts against Methicillin-resistant Staphylococcus aureus

  • Joung, Dae Ki (Department of Development in Oriental Medicine Resources, Sunchon National University) ;
  • Kwon, Dong Yeul (Department of Oriental Pharmacy, Wonkwang University) ;
  • Shin, Dong Won (Department of Development in Oriental Medicine Resources, Sunchon National University)
  • Received : 2018.08.06
  • Accepted : 2018.12.20
  • Published : 2018.12.31
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Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is a bacterium responsible for a number of infections in humans that are difficult to treat, and as a result, is a substantial contributor to morbidity and mortality. In the present study, in search of natural products capable of inhibiting this multidrug-resistant bacterium, we investigated the antimicrobial activity of Lysimachia clethroides Duby root. The antibacterial activities of EtOH extract of Lysimachia clethroides Duby root and its n-hexane, EtOAc, n-BuOH and water fractions were evaluated against 15 strains of methicillin-resistant staphylococcus aureus (MRSA) and 1 standard methicillin-susceptible S. aureus (MSSA) strain by using the minimal inhibitory concentrations (MICs) assay, colorimetric assay using MTT test, checkerboard dilution test. Antimicrobial activity of n-hexane fraction of Lysimachia clethroides Duby root was remarkable. Against the 16 strains, the minimum inhibitory concentrations (MICs) were in the range of $31.25-62.5{\mu}g/ml$ and FICI values for n-hexane fraction of Lysimachia clethroides Duby root+AM and n-hexane fraction of Lysimachia clethroides Duby root+OX were checkerboard method performed using the MRSA, MSSA and one clinical isolate strains via MICI 0.12-1 and 0.25-0.75, showing the increase of synergistic effect. When combined together, these antibiotic effects were dramatically increased. These effective combinations could be new promising agents in the management of MRSA.

Keywords

Table 1. The S. aureus strains used in the experiments

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Table 2. Antimicrobial activity of Lysimachia clethroides Duby root ethanol extract, n-hexane, EtOAc, n-BuOH and water fractions against S. aureus strains under dark

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Table 3. Result of the combined effect of n-hexane fraction of Lysimachia clethroides Duby root and AM against S. aureus

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Table 4. Result of the combined effect of n-hexane fraction of Lysimachia clethroides Duby root and OX against S. aureus

JOSMBA_2018_v31n6_719_t0004.png 이미지

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