Fig. 1. Diagrammatic representation of experimental design for gene fow. Genetically modifed (GM) soybeans were planted in center (black circle) as pollen source. non-GM soybeans (white circle) were planted in four directions beside GM soybeans.
Fig. 2. The experimental design of the field trial. Genetically modified (GM) soybeans were planted in center (black rectangles) as pollen source. non-GM soybeans were planted in four directions beside GM soybeans. The size of GM pollen source was 90 cm × 90 cm (A), 180 cm × 180 cm (B), and 360 cm × 360 cm (C). (D) is photograph of the experimental field trial.
Fig. 3. The example of sampling point for gene flow. Gray squares indicate the soybeans from which fruits were collected for screening hybrids.
Fig. 4. Part of hybrid detection by PCR analysis. bar gene was used to detect hybrid and lectin gene used as control gene. GM, genetically modifed. * means non-specifc band.
Table 1. Frequency of out-crossing rates evaluated from surviving progeny in East and West sites.
Table 2. Frequency of out-crossing rates evaluated from surviving progeny in North and South sites.
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