Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
권호 표지
DOI QR코드

DOI QR Code

Distribution of Lawsonia intracellularis in livestock transport car of slaughterhouse, Korea

도축장 출하차량에서 Lawsonia intracellularis 분포율 조사

  • Lee, Su-Ji (North-Branch, Jeonbuk Veterinary Service Laboratory) ;
  • Lee, Hee-Seon (North-Branch, Jeonbuk Veterinary Service Laboratory) ;
  • Seo, Ji-Soo (North-Branch, Jeonbuk Veterinary Service Laboratory) ;
  • Kim, Tae-Gyeom (North-Branch, Jeonbuk Veterinary Service Laboratory) ;
  • Jeong, Jae-Kyo (North-Branch, Jeonbuk Veterinary Service Laboratory)
  • 이수지 (전라북도동물위생시험소 남부지소) ;
  • 이희선 (전라북도동물위생시험소 남부지소) ;
  • 서지수 (전라북도동물위생시험소 남부지소) ;
  • 김태겸 (전라북도동물위생시험소 남부지소) ;
  • 정재교 (전라북도동물위생시험소 남부지소)
  • Received : 2018.05.27
  • Accepted : 2018.12.01
  • Published : 2018.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

Lawsonia intracellularis is the pathogenic agent of porcine proliferative enteritis (PPE). The bacterial pathogen infects the intestinal crypt cells which causes hyperplasia of the infected cells and leads to the process of intestinal pathogenesis. PPE includes some clinical maninfestations, including acute hemorrhagic diarrhea with sudden death in growing pigs and porcine intestinal adenomatosis, to a chronic diarrhea with reduced productivity of the infected pigs. The purpose of the present studies were carried out to determine L. intracellularis in livestock transport car of slaughterhouse. Distribution of L. intracellularis in livestock transport car were conducted using real-time polymerase chain reaction (real-time PCR) testing method, total 300 samples. Of 300 samples, 119 (39.7%) were detected as positive to L. intracellularis in livestock transport car. In seasonal analysis, 42 (28.0%) out of 150 samples in spring and summer season. 77 (51.3%) out of 150 sample in autumn and winter season. In regional analysis, 53 (88.3%) out of 60 cars and the detection ratio showed that regional variation in livestock transport car.

Keywords

Table 1. Isolates of L. intracellularis from livestock transport car

GCOSBX_2018_v41n4_245_t0001.png 이미지

Table 2. Monthly detection rates of L. intracellularis using real-time PCR

GCOSBX_2018_v41n4_245_t0002.png 이미지

Table 3. Seasonal detection rates of L. intracellularis by real-time PCR

GCOSBX_2018_v41n4_245_t0003.png 이미지

Table 4. Regional differences in the detection rate of L. intracellularis

GCOSBX_2018_v41n4_245_t0004.png 이미지

References

  1. 농림축산식품부. 2017. 2016/2017년 고병원성 조류인플루엔자 역학조사 분석 보고서. pp. 55-126.
  2. 농림축산식품부. 2017. 2017 구제역 역학조사 분석보고서. pp. 61-67.
  3. Cooper DM, Swanson DL, Gebhart CJ. 1997. Diagnosis of proliferative enteritis in frozen and formalin-fixed, paraffin embedded tissue from a hamster, horse, deer and ostrich using a Lawsonia intracellularis specific multiplex PCR assay. Vet Microbiol 54: 47-62. https://doi.org/10.1016/S0378-1135(96)01264-3
  4. Gebhart CJ, Barns SM, McOrist S, Lin GF, Lawson GH. 1993. Ileal symbiont intracellularis, an obligate intracellular bacterium of porcine intestines showing a relationship to Desulfovibrio species. Int J Syst Bacteriol 43: 553-538.
  5. Gebhart CJ, Ward GE, Murtaugh MP. 1989. Species-specific cloned DNA probe for identification of Campylobacter hyointestinalis. J Clin Microbilol 27: 2717-2723.
  6. Guedes RM, Gebhart CJ, Winkelman NL, Mackie-Nuss RA. 2002. A comparative study of an indirect fluorescent antibody test and an immunoperoxidase monolayer assay for the diagnosis of porcine proliferative enteropathy. J Vet Diagn Invest 14: 420-423. https://doi.org/10.1177/104063870201400512
  7. Jones GF, Ward GE, Collins JE, Gebhart CJ. 1993a. Transmission of proliferative enteritis to swine by use of embryonating chicken eggs. Am J Vet Res 54: 1256-1261.
  8. Jones GF, Ward GE, Murtaugh MP, Lin G, Gebhart CJ. 1993b. Enhanced detection of intracellular organism of swine proliferative enteritis, ileal symbiont intracellularis, in feces by polymerase chain reaction. J Clin Microbiol 31: 2611-2615.
  9. Jung YS, Park YR, Kang DY, Han DH, Yoon D, Jung BY, Park CK. 2016. Prevalence of major enteric pathogens in different feeding groups of pig in Korean pig farms. Korea. J Vet Sci 39: 211-219.
  10. Lawson GH, McOrist S, Jasni S, Mackie RA. 1993a. Intracellular bacteria of porcine proliferative enteropathy; cultivation and maintenance in vitro. J Clin Microbiol 31: 1136-1142.
  11. Lawson GH, McOrist S. 1993b. The enigma of the proliferative enteropathies: a review. J Comp Pathol 108: 41-46. https://doi.org/10.1016/S0021-9975(08)80225-3
  12. Lee SW, Kim TJ, Park SY, Song CS, Chang HK, Yeh JK, Park HI, Lee JB. 2001. Prevalence of porcine proliferative enteropathy and its control with tylosin in Korea. J Vet Sci 2: 209-212. https://doi.org/10.4142/jvs.2001.2.3.209
  13. Lym SK, Lee HS, Woo SR, Yoon SS, Moon OK, Lee YY, Koh HB. 1999. Establishment of diagnostic method for porcine proliferative enteropathy using polymerase chain reaction. Korean J Vet Res 39: 118-125.
  14. McCormick BM, Hasse D, Monkton RP. 1995. Detection of ileal symbiont intracellularis in porcine faecal sample by polymerase chain reaction. Vet Microbiol 47: 387-393. https://doi.org/10.1016/0378-1135(95)00118-2
  15. McOrist S, Boid R, Lawson GH, McConnell I. 1987. Monoclonal antibodies to intracellular Campylobacter-like organisms of the porcine proliferative enteropathies. Vet Rec 121: 421-422. https://doi.org/10.1136/vr.121.18.421
  16. McOrist S, Gebhart CJ, Lawson GH. 1994. Polymerase chain reaction for diagnosis of porcine proliferative enteropathy. Vet Microbiol 41: 205-212. https://doi.org/10.1016/0378-1135(94)90101-5
  17. McOrist S, Gebhart CJ. 1999. Porcine proliferative enteropathies. pp. 727-737. In: Straw BE, D'Allaire S, Mengeling WL, Taylor DJ(Eds). Disease of Swine. 9th. Wiley-Blackwell. Ames. Iowa.
  18. McOrist S, MacIntyre N, Stokes CR, Lawson GH. 1993. Relationship between ileal symbiont intracellularis and porcine proliferative enteritis. Infect Immun 60: 4184-4191.
  19. McOrist S, Mackie RA, Neef N, Aitken I, Lawson GH. 1994. Synergism of ileal symbiont intracellularis and gut bacteria in the reproduction of porcine proliferative enteropathy. Vet Rec 134: 331-332. https://doi.org/10.1136/vr.134.13.331
  20. Na DH, Hong YH, Yoon MY, Park EJ, Lim TH, Jang JH, Kim BY, Lee DH, Song CS. 2013. Prevalence of Salmonella Species Isolated from Old Hen Delivery Trucks in Korea and Application of Disinfectant for the Reduction of Salmonella Contamination. Korean J Poult Sci 40: 11-16. https://doi.org/10.5536/KJPS.2013.40.1.011
  21. Park DY, Park AR, Jung EH, Bae JH, LeeGC, Hwang BW, Lee MK. 2009. Survey of porcine proliferative enteritis for the pig farms in Gyeongnam district. Korean J Vet Serv 32: 361-368.
  22. Roberts L, Rowland AC, Lawson GHK. 1977. Experimental reproduction of porcine intestinal adenomatosis and necrotic enteritis. Vet Rec 100: 12-13. https://doi.org/10.1136/vr.100.1.12
  23. Roerink F, Morgan CL, Knetter SM, Passat MH, Archibald AL, Ait-Ali T, Strait EL. 2018. A novel inactivated vaccine against Lawsonia intracellularis induces rapid induction of humoral immunity, reduction of bacterial shedding and provides robust gut barrier function. Vaccine 36: 1500-1508. https://doi.org/10.1016/j.vaccine.2017.12.049
  24. Smith SH, McOrist S. 1997. Development of persistent intestinal infection and excretion of Lawsonia intracellularis by piglets. Res Vet Sci 60: 6-10.
  25. Ward GE, Jones GF. 1991. Use of embryonating eggs for isolation of Campylobacter species from intestine of swine with proliferative enteritis. Am J Vet Res 52: 810-812.