한국어병학회지 (Journal of fish pathology)

  • 제31권2호
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  • Pages.87-92
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  • 2018
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  • 1226-0819(pISSN)
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  • 2233-5412(eISSN)
한국어병학회 (The Korean Society of Fish Pathology)
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우리나라 양식 자주복, Takifugu rubripes (Temminck & Schlegel, 1850)에서 발생한 Vibrio atlanticus의 첫 감염 사례 보고

The first case report on Vibrio atlanticus infection in cultured tiger puffer, Takifugu rubripes

  • 이남실 (국립수산과학원 병리연구과) ;
  • 조미영 (국립수산과학원 병리연구과) ;
  • 정승희 (국립수산과학원 병리연구과) ;
  • 원경미 (국립수산과학원 병리연구과)
  • Lee, Nam-Sil (Fish Pathology division, National Fisheries Research and Development Institute) ;
  • Cho, Miyoung (Fish Pathology division, National Fisheries Research and Development Institute) ;
  • Jung, Sung-hee (Fish Pathology division, National Fisheries Research and Development Institute) ;
  • Won, Kyoungmi (Fish Pathology division, National Fisheries Research and Development Institute)
  • 투고 : 2018.10.08
  • 심사 : 2018.11.23
  • 발행 : 2018.12.31
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초록

본 연구는 육상 순환 양식장, 약 $20^{\circ}C$의 수온에서 양식 중이던 자주복에서 체표에 백탁과 궤양을 보이는 개체가 다수 발생하여 그 원인을 밝히고자 하였다. TCBS 선택배지에서 순수 배양된 균체를 확인하고 계대 배양하여 16S rRNA와 rpoA 유전자의 염기서열 분석을 실시하였으며, 그 결과는 Vibrio atlanticus와 99.77%의 상동성을 가지는 것으로 나타났다. 생화학적 특징을 이미 보고된 같은 균종과 비교한 결과, Voges-Proskauer test와 Gelatin 가수분해능에 대한 결과에서 차이를 나타내는 것으로 확인하였다.

A case of disease was occurred in tiger puffer, Takifugu rubripes, reared in circulated system (water temperature was about $20^{\circ}C$) on land in Korea. Diseased fish showed a unique external sign, the cloudy skin ulcer in various sizes. We investigated the cause of disease, and isolated one pure cultured bacterium, TP01. This had 99.7% homology with Vibrio atlanticus by sequence analysis of the 16S rRNA and rpoA genes. TP01 showed some differences with other reported V. atlanticus strains in the biochemical characteristics as like Voges-Proskauer test and gelatin hydrolysis.

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참고문헌

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