Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Genetic Diversity of Two Isolates in Strawberry mild yellow edge virus from Korea

국내에서 분리한 딸기누른오갈바이러스의 유전적 다양성

  • Kwon, Sun-Jung (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Cho, In-Sook (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Yoon, Ju-Yeon (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Choi, Gug-Seoun (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, RDA)
  • 권선정 (농촌진흥청국립원예특작과학원원예특작환경과) ;
  • 조인숙 (농촌진흥청국립원예특작과학원원예특작환경과) ;
  • 윤주연 (농촌진흥청국립원예특작과학원원예특작환경과) ;
  • 최국선 (농촌진흥청국립원예특작과학원원예특작환경과)
  • Received : 2018.10.14
  • Accepted : 2018.11.04
  • Published : 2018.12.31
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Abstract

Two isolates of Strawberry mild yellow edge virus were newly isolated in strawberry (Fragaria x ananassa) cultivar Selhyang and Kamhong from Korea. The complete nucleotide sequence of the coat protein (CP) of two Korean isolates were determined and analyzed. Sequence identity of nucleotide and amino acid between SH and KH isolates was 90.4% and 95.5%, respectively. The comparison of three Korean isolates including previously reported KNS1 with 45 SMYEV sequences from other countries deposited in GenBank database revealed an identity ranging from 81.2% to 100%. The phylogenetic analysis of CP of all SMYEV isolates showed the five subgroups (I-V), with Korean isolates being divided into two different subgroups. The isolates KH and KNS1 were included in subgroup I, whereas SH was included in subgroup IV which is new phylogenetic subgroup. Genetic diversity analysis indicated that new subgroup had greater variability and nucleotide diversity between subgroups resulted in values ranging from 0.0863 to 0.18004. This report represents the first molecular characterization of SMYEV isolates from Korea.

국내육성딸기 품종인 설향과 감홍에서 딸기누른오갈바이러스의 국내 분리주 2종을 분리하고 외피단백질 전체 염기서열을 결정하고 분석하였다. 국내 분리주 SH와 KH의 외피단백질 염기와 아미노산 상동성은 각각 90.4%와 95.5% 였다. 기존에 국내에서 보고된 KNS1분리주와 GenBank에 등록된 45개의 다른나라 분리주 외피단백질 염기서열을 모두 수집하여 총 48개 SMYEV 외피단백질에 대한 계통학적 유연관계를 분석할 결과 총 5개의 subgroup (I-V)으로 분류가 되었다. 이 중 subgroup IV과 V과 새로운 변이집단으로 국내분리주도 KH와 KNS1은 subgroup I에 포함된 반면, SH는 새로운 subgroup인 IV에 포함되어 국내분리주간에도 계통이 다른 것을 추측할 수 있었다. 유전적 다양성 분석결과 SMYEV의 새로운 subgroup의 다양성이 더욱 높은 것으로 나타나 SMYEV가 유전적으로 진화를 하고 있음을 알 수 있었다. 이 논문은 국내 SMYEV 분리주에 대한 분자적 특성에 대한 첫 보고이다.

Keywords

SMBRCU_2018_v24n4_285_f0001.png 이미지

Fig. 1. Alignment of the complete amino acid (aa) sequences of the coat protein of 3 SMYEV Korean isolates. Sequences were aligned by ClustalX2. Consensus aa sequences are located line below. Only those residues differing from consensus are shown for the isolates. Region underlined is the most conserved motifs (FAAFDFF at residue 168-174) shared between SMYEV isolates.

SMBRCU_2018_v24n4_285_f0002.png 이미지

Fig. 2. Phylogenetic analysis of the complete nucleotide sequences of the coat protein of different SMYEV isolates (Table 1). Three Korean isolates are marked with arrows. The Potato virus X (PVX; GenBank accession No. NC011620) was included as an outgroup. The phylogenetic trees were reconstructed by the NJ method applying Kimura’s two-parameter method. The numbers on the branches indicate bootstrap percentages based on 1000 replications (only values >60% are shown). The scale bar represents the number of substitutions per base.

Table 1. Information of SMYEV isolates available in the NCBI compared in this study

SMBRCU_2018_v24n4_285_t0001.png 이미지

Table 2. Average percent nucleotide sequence and amino acid identities (number in bracket) between the three Korean isolates and the five phylogenetic groups of SMYEV

SMBRCU_2018_v24n4_285_t0002.png 이미지

Table 3. Nucleotide divergence of CP gene within and between subgroups of all SMYEV isolates

SMBRCU_2018_v24n4_285_t0003.png 이미지

Table 4. Information about the polymorphism of SMYEV isolate subgroups

SMBRCU_2018_v24n4_285_t0004.png 이미지

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