고추장과 발효액이 첨가된 알래스카 산 프리미엄 명란의 ABTS, FRAP, total phenolic acid의 항산화 특성 분석

Antioxidant Properties (ABTS, FRAP, Total Phenolic Content) of Alaska and Gochujang Pollock Roes and Fermented Pollock Roe Seasoning

Abstract

알래스카 산 대구(Gadus chalcogrammus)는 북태평양에 분포하는 대구과 한류 어종이다. 알래스카 산 대구의 명란 원료, 프리미엄 고추장 명란, 프리미엄 발효 명란을 증류수와 에탄올로 추출하여 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS+), 철 환원 항산화능(Ferric Reducing Antioxidant Potential, FRAP), 총 페놀함량을 평가하였다. 명란 원료의 증류수와 에탄올 추출물의 ABTS+ 소거능은 1.0 mg/ml 농도에서 각각 50.1%, 53.1%였다. 고추장 명란의 증류수와 에탄올 추출물의 ABTS+ 소거능은 1.0 mg/ml 농도에서 각각 71.3%, 71.6%였다. 발효 명란의 증류수와 에탄올 추출물의 ABTS+ 소거능은 1.0 mg/ml 농도에서 각각 68.7%, 70.4%였다. 명란원료의 증류수와 에탄올 추출물의 ABTS+에 대한 최소 저해 농도 값($EC_{50}$)은 각각 12.49 ug/ml과 12.21 ug/ml이었다. 고추장 명란의 증류수와 에탄올 추출물의 FRAP에 대한 최소 저해 농도 값($EC_{50}$)은 각각 10.67 ug/ml과 10.56 ug/ml이었다. 발효 명란의 증류수와 에탄올 추출물의 총 페놀 함량에 대한 최소 저해 농도 값($EC_{50}$)은 각각 10.45 ug/ml과 10.31 ug/ml이었다. Gallic acid를 대조구로 할 때 총 페놀 함량에 대한 상대적 활성은 명란 원료가 52.0%, 고추장 명란이 61.1%, 발효 명란이 63.6%였다. 명란 원료보다 고추장 명란과 발효 명란에서 ABTS+, FRAP 소거능 및 총 페놀 함량이 우수하였다.

The Alaska Pollock (Gadus chalcogrammus) is distributed in an arc across the North Pacific Ocean. Distilled water extracts (DWE) and ethanol extracts (ETE) of 1.0 mg/ml concentrations of raw Alaska Pollock roe, premium Gochujang Pollock roe, and premium fermented Pollock roe seasoning were evaluated for estimated 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), ferric reducing antioxidant potential (FRAP), and total phenolic content. The ABTS scavenging activity of the raw Alaska roe DWE and ETE were evaluated at 50.1% and 53.1%, respectively. The ABTS scavenging activity of the Gochujang roe DWE was 68.7% and of the ETE was 70.4%; for the fermented seasoning it was 71.3% and 71.6% for the DWE and ETE, respectively. The ABTS $EC_{50}$ values of the raw roe DWE and ETE were 12.49 ug/ml and 12.21 ug/ml, respectively. The FRAP $EC_{50}$ values of the Gochujang roe DWE and ETE were 10.67 ug/ml and 10.56 ug/ml, respectively, and the $EC_{50}$ values for total phenolic content for the fermented seasoning DWE and ETE were 10.45 ug/ml and 10.31 ug/ml, respectively. When Gallic was acid used as a control, the relative total phenolic content scavenging activity in each ETE was 52.0% (raw Alaska roe), 61.1% (Gochujang roe), and 63.6% (fermented seasoning). In the present study, higher ABTS, FRAP, and total phenolic content were observed in the Gochujang Pollock roe and fermented roe seasoning than in the Alaska Pollock roe.

Fig. 1. Relative inhibitory effects on ABTS+ by ethanol extracts from various Pollock roes.

Fig. 2. Relative inhibitory effects on FRAP by ethanol extracts from various Pollock roes.

Fig. 3. Relative inhibitory effects on total phenolic content by ethanol extracts from various Pollock roes.

Table 1. The assay of 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) scavenging activity of premium Pollock roe at different concentrations

Table 2. The ferric reducing antioxidant potential (FRAP) assay of premium Pollock roe at different concentrations

Table 3. The assay of total phenolic content of premium Pollock roe at different concentrations

Table 4. The 50% inhibition (EC₅₀) of ABTS+, FRAP, and total phenolic content of Alaska Pollock roe at different solvents