The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology (한방안이비인후피부과학회지)

The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology (대한한방안이비인후피부과학회)
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Effects of Shinsuwisaengtang on Antioxidant Activity and Inhibition of Phototoxicity by UVB Irradiation

신수위생탕(神授衛生湯)이 UVB 조사에 의한 광독성억제활성에 대한 효과연구

  • Kim, Ee-Hwa (Dept. of Meridian & Acupoint, College of Korean Medicine, Semyung University) ;
  • Kim, Yong-Min (Dept. of Cosmetic Science & Beauty Biotechnology, Semyung University) ;
  • Kim, Hee-Taek (Dept. of Korean Medical Ophthalmology & Otolaryngology & Dermatology, College of Korean Medicine, Semyung University)
  • 김이화 (세명대학교 한의과대학 경락경혈학교실) ;
  • 김용민 (세명대학교 화장품.뷰티생명공학부) ;
  • 김희택 (세명대학교 한의과대학 한방안이비인후피부과학교실)
  • Received : 2017.12.28
  • Accepted : 2018.02.12
  • Published : 2018.02.25
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Abstract

Objectives : The purpose of this study is to investigate the effect of Shinsuwisaengtang (SSWST) extracts on antioxidant activity and inhibition of phototoxicity as a medicine for skin damage due to ultra violet stimulation. Methods : To determine the cytotoxicity of Hs68 cells from SSWST extracts, we investigated cell viability by MTT assay. To determine the protective effect of phototoxicity, we investigated cell viability after UVB radiation. The DPPH radical scavenging activity was examined to determine the antioxidant effect of SSWST extracts. Hoechst 33258 staining was performed to observe the protective effect of SSWST on UVB. Results : The cytotoxicity of SSWST extracts in Hs68 cells was not appeared significantly in all concentration. SSWST extracts significantly increased the viability of UVB-stimulated Hs68 cells at a concentration of 25, 50 and $100{\mu}g/ml$. SSWST extract showed higher DPPH radical scavenging than the control group. It was observed that SSWST extracts inhibited the apoptosis of the UVB-stimulated Hs68 cells through a fluorescence microscope. Conclusions : It was observed that SSWST did not induce cytotoxicity at a constant concentration and had a protective effect on phototoxicity and an antioxidant effect. Thus, it is considered that it maybe used as a medicine to cure skin damage caused by UVB and photoaging changes in the future.

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References

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