대한한방부인과학회지 (The Journal of Korean Obstetrics and Gynecology)

  • 제31권1호
  • /
  • Pages.20-42
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  • 2018
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  • 1229-4292(pISSN)
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  • 2508-3619(eISSN)
대한한방부인과학회 (The Society of Korean Medicine Obstetrics and Gynecology)
권호 표지
DOI QR코드

DOI QR Code

Ob/Ob 마우스에서 계지복령환(桂枝茯苓丸)과 계지복령환(桂枝茯苓丸) 합강지환(合降脂丸)이 비만 및 지방대사에 미치는 영향

Effect of Geijibokryung-hwan and Combination of Geijibokryung-hwan and Gangji-hwan on Obesity and Lipid Metabolism in Ob/Ob Mice

  • 김민애 (동의대학교 한의과대학 부인과교실) ;
  • 송정오 (동의대학교 한의과대학 부인과교실) ;
  • 이인선 (동의대학교 한의과대학 부인과교실)
  • Kim, Min-Ae (Dept. of OB & GY, College of Oriental Medicine, Dong-Eui University) ;
  • Song, Jung-Oh (Dept. of OB & GY, College of Oriental Medicine, Dong-Eui University) ;
  • Lee, In-Seon (Dept. of OB & GY, College of Oriental Medicine, Dong-Eui University)
  • 투고 : 2018.01.24
  • 심사 : 2018.02.14
  • 발행 : 2018.02.23
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초록

Objectives: This study was designed to investigate anti-obesity effects the improvement effects of Gyejibongnyeong-hwan and Gyejibongnyeong-hwan-Gangji-hwan (CIPPDF) in a ob/ob mouse model. Methods: Seven-week old mice (wild-type C57BL/6J and ob/ob) were used for all experiments. Wild-type C57BL/6J mice were used as normal group and obese ob/ob mice were randomly divided into 4 groups. a normal group given a standard diet, an obese control group given a standard diet with CIPP (300 mg/kg), CIPPDF (1) (300+300 mg/kg), CIPPDF (2) (300+600 mg/kg) respectively. After 10 weeks of treatment, body weight gain, feeding efficiency ratio, blood lipid markers, mRNA levels of genes involved in fatty acid ${\beta}-oxidation$ and lipogenesis in in-vivo, were examined. Results: 1. Body weight gain and Feeding efficiency ratio were significantly decreased in CIPPDF (1) compared with control. Fat mass was significantly decreased in CIPPDF (2) in EAT compared with control. 2. Consistent their effects on body weight gain and fat mass, circulating concentrations of LDL-cholesterol were decreased in CIPPDF (1), CIPPDF (2) groups compared with control. 3. MCAD mRNA levels of genes was increased in CIPPDF (1), CIPPDF (2) groups in the liver, epididymal adipose tissue compared with control. VLCAD mRNA levels of genes was increased in CIPPDF (1), CIPPDF (2) groups in the skeletal muscle compared with control. 4. $PPAR{\gamma}$ mRNA was decreased in CIPPDF (1) in the liver compared with control. SCD1 mRNA was decreased in CIPPDF (1), CIPPDF (2) groups in the epididymal adipose tissue compared with control. Conclusions: In conclusion, These results suggest that CIPPDF not only decrease feeding efficiency ratio, and LDL-cholesterol, but also reduce EAT fat mass contributing to the improvement of ovesity. CIPPDF also were increased in mRNA levels of genes involved in fatty acid ${\beta}-oxidation$ and decreased in mRNA levels of genes involved in lipogenesis.

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참고문헌

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