꽃매미 알 기생천적인 꽃매미벼룩좀벌의 대량사육기술: 산누에나방과 꽃매미 알 활용 방법

Mass-rearing Techniques of Anastatus orientalis (Hymenoptera: Eupelmidae), as the Egg-parasitoid of Lycorma delicatula (Hemiptera: Fulgoridae): An Using Method of Antheraea pernyi (Lepidoptera: Saturniidae) and L. delicatula Eggs in Laboratory

Abstract

꽃매미벼룩좀벌(Anastatus orientalis)을 실내에서 대량사육 할 수 있는 대체기주를 선발하기 위하여 꽃매미와 썩덩나무노린재(Halyomopha halys), 톱다리개미허리노린재(Riptortus pedestris), 매미나방(Lymantria dispar), 참나무산누에나방(Antheraea yamamai), 귀뚜라미(Verlarifictorus spp.), 집파리(Musca domestica), 산누에나방(Antheraea pernyi) 총 8 종의 알 또는 번데기를 이용하여 검토하였다. 그 결과 꽃매미 알과 산누에나방의 미성숙 알에서는 꽃매미벼룩좀벌의 산란과 우화가 확인되었으나 그 밖에 종의 알과 번데기에서는 산란을 확인할 수 없었다. 대체기주로 선발된 산누에나방은 참나무 잎으로 사육하였으며, 7월과10월 상순에 번데기를 수확하여 우화 후 4~5일경에 복부가 불룩한 산란 직전의 암컷 성충만을 선발하여 $1{\sim}5^{\circ}C$ 냉장고에 보관하였으며, 저온에 보관했던 암컷의 복부를 절개하여, 암컷 한 마리당 150~200개의 알을 얻었다. 꽃매미 알과 산누에나방의 미성숙 알에서 꽃매미벼룩좀벌의 발육기간은 각각 평균 36.8일과 36.1일이었으며, 24시간동안 암컷 한 마리가 산란한 알의 수는 각각 평균 4.2개와 3.4개로 통계적으로는 유의한 차이가 없었다. 한편, 꽃매미벼룩좀벌 암컷 성충의 수명은 벌꿀을 먹이로 제공 하였을 때, 평균 64.3일까지 생존하는 것으로 확인되었다. 산누에나방의 미성숙 알을 대량으로 확보하고 벌꿀을 먹이로 제공하면 꽃매미벼룩좀벌을 실내에서 대량사육 하는 것이 가능하고 야외방사를 통해 꽃매미의 생물적 방제에 활용할 수 있을 것으로 기대된다.

Eggs, immature eggs, and pupae of 8 different insects (Halyomopha halys, Riptortus pedestris, Lymantria dispar, Antheraea yamamai, Verlarifictorus spp, Antheraea pernyi, and Musca domestica) including Lycorma delicatura were used to select the alternative host for laboratory mass rearing of A. orientalis. Except L. delicatula's eggs and immature eggs of A. pernyi, other 7 tested insects were not parasitized by A. orientalis. A. pernyi was reared with oak tree leaves and its cocoons were harvested on mid-July and early October. On 4 or 5 days after emergence, only female adults showing swollen abdomen were collected and stored at $1{\sim}5^{\circ}C$. We could get 150~200 eggs per one female by dissecting the female's abdomen. For examining the possibility for laboratory mass rearing of A. orientalis with A. pernyi's immature eggs, developmental periods from egg to pupa between the two different hosts were compared. Developmental periods were 36.1 days on immature eggs of A. pernyi and 36.8 days on an original host's eggs, respectively. The number of parasitized eggs by A. orientalis' female for 24 h was 3.4 on immature eggs of A. pernyi and 4.2 on an original host's eggs, respectively. However, there were no significant statistical differences in developmental period and parasitization between the two hosts. By supplying honeyed water to newly emerged female parasitoids, it was able to maximize their longevities up to 64.3 days after emergence. Therefore, our results support potential for laboratory mass-rearing of A. orientalis using A. pernyi's immature eggs as an alternative host.

Fig. 1. Eggs and pupae used for selecting alternative host insects for laboratory mass rearing of A. orientalis. (A) Halyomopha halys, (B) Riptortus pedestris, (C) Lymantria dispar, (D) Antheraea yamamai, (E) Verlarifictorus spp, (F) Musca domestica, (G) Antheraea pernyi eggs, (H) Antheraea pernyi immature eggs (I) Lycorma delicatula

Fig. 2. Mass-rearing process of Antheraea pernyi adults and immature eggs as an alternative host of L. delicatula. (A) newly emerged A. pernyi adult, (B) eggs, (C) rearing of eggs on Quercus variabilis, (D) collection of pupae on Q. variabilis, (E) selection of females after emergence, (F) storage of females at 1~5℃, (G) abdominal amputation, (H) vertical dissection of abdomen, (I) egg mass, (J) washing the egg mass on the sieve with tap water , (K) drying on the fine mesh , (L) Storage of dried eggs at 1~5℃.

Fig. 3. Laboratory mass-rearing of A. orientalis using A. pernyi ’s immature eggs. (A) released A. orientalis adults on the alternative host eggs, (B) female wasp’s oviposition, (C) host feeding, (D) feeding and oviposition trace on the surface of host eggs, (E) emergence from the hole, (F) supply the honeyed water and host eggs as preys to the emerged adults.

Fig. 4. Developmental periods (egg to pupa) of A. orientalis on eggs of the two insects, L. delicatula and A. pernyi. NS indicates no significant difference (P = 0.074) between the two insects.

Fig. 5. Number of eggs oviposited by A. orientalis female within 24 hours between the two insects, L. delicatula and A. pernyi. NS indicates no significant difference (P = 0.563) between the two insects.

Table 1. Eggs and pupae used for selecting alternative host insects for laboratory mass rearing of A. orientalis

Table 2. Longevities of A. orientalis adults on two different preys