DOI QR코드

DOI QR Code

포제 및 발효 가공에 따른 오미자와 구기자 물 추출물의 항염증 및 숙취해소 효과

Anti-inflammation and hangover relief effects of Schisandra chinensis (SC) and Lycium chinense (LC) water extracts depending on drug processing and fermentation

  • 김하림 ((재)전주농생명소재연구원) ;
  • 김상준 ((재)전주농생명소재연구원) ;
  • 김솔 ((재)전주농생명소재연구원) ;
  • 김홍준 (우석대학교 한의과대학) ;
  • 정승일 ((재)전주농생명소재연구원) ;
  • 유강열 ((재)전주농생명소재연구원) ;
  • 김선영 ((재)전주농생명소재연구원)
  • 투고 : 2018.11.12
  • 심사 : 2018.11.28
  • 발행 : 2018.11.30

초록

Schisandra chinensis (SC) and Lycium chinense (LC) were widely distributed in Asia and the fruit has been used traditionally for medicinal herbs. The processing method was solid-state fermentation using Aspergillus oryzae for 48 h after stir-frying treatment at $220^{\circ}C$ for 12 min. In this study, in vitro the anti-inflammatory effect and in vivo hangover reduction were compared to unprocessed SC and LC water extract. Anti-inflammatory effects have been evaluated in pro-inflammatory mediators which were secreted by lipopolysaccharide (LPS)-induced RAW 264.7 macrophages. Nitric oxide (NO) was determined using Griess reaction. Proinflammatory cytokines such as tumor necrosis factor $(TNF)-{\alpha}$ and interleukin $(IL)-1{\beta}$ were measured by enzyme-linked immunosorbent assays (ELISA). Alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) activities were compared to processed SC or LC and mixtures thereof (1:1). In vivo study was compared to hangover relief in alcohol-fed mice. After administering a mixture of SC and LC (300 mg/kg) water extract (1:1), mice were fed 3 g/kg of ethanol. Serum was collected at 1, 3, and 5 h intervals to analyze ethanol and acetaldehyde levels using a colorimetric assay kit. The processed SC and LC water extracts compared to raw materials significantly inhibited LPS-induced NO and inflammatory cytokine production in RAW 264.7 cells. The results of the hangover mouse model are also consistent with anti-inflammatory effects. These results suggest that processed SC and LC extracts may be functional materials for the treatment of inflammation and hangover.

키워드

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Fig. 1. Inhibitory effects of processed Schisandra chinensis (SC) and Lycium chinense (LC) on nitric oxide (NO) and inflammatory cytokines production in RAW 264.7 cell.

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Fig. 2. Comparison results for the processed SC and LC products against on Alcohol dehydrogenase (ADH) and Aldehyde dehydrogenase (ALDH) activation.

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Fig. 3. Effects of processed SC and LC mixture on relieving hangover in mice administered alcohol.

Table 1. The classification of group

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Table 2. ABTS radical scavenging activity, DPPH radical scavenging activity and SOD activity of Schisandra chinensis (SC) and Lycium chinense (LC)

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Table 3. Effects of processed SC and LC mixture on serum ADH activity.

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