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Food Value of Cyclopoid Copepod, Paracyclopina nana for Flounder Paralichthys olivaceus Larvae

넙치자어에 대한 cyclopoid copepod, Paracyclopina nana 의 먹이효과

  • Lee, Kyun-Woo (Marine Biotechnology Research Center, Korea Institute of Ocean Science & Technology)
  • Received : 2018.10.04
  • Accepted : 2018.11.02
  • Published : 2018.11.30
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Abstract

This study investigated the food value of Paracyclopina nana for flounder Paralichthys olivaceus larvae in two feeding stages, rotifer (for 12 days) and Artemia (for 16 days). In the rotifer feeding stage, survival and growth of flounder larvae in the only P. nana (nauplii) feeding experiment were higher than in the only rotifer feeding experiment on 12 DAH (days after hatching). In the Artemia feeding stage, the growth of flounder larvae in the only P. nana (C4-adult) feeding experiment and mixture feeding experiment (P. nana+Artemia) were higher than in other experiments on 30 DAH, but the survival of flounder larvae did not differ significantly among experiments. The n-3 HUFA contents of nauplius and C4-adult were 4.0% and 5.4%, respectively. Overall the results of this study indicate that the brackish water cyclopoid copepod, P. nana, is an effective live food organism for larval seedling production of marine fish.

본 연구는 넙치 (Paralichthys olivaceus) 자어에 대한 요각류 Paracyclopina nana의 먹이가치를 평가하기 위해 두섭식 단계 (rotifer 섭식 단계와 Artemia 섭식 단계)로 나누어 각각 12일 및 16일 동안 수행되었다. Rotifer 섭식 단계 실험에서, 넙치 자어의 생존과 성장은 부화 12일째 rotifer만 공급했을 때 보다 P. nana (nauplii) 단독으로 공급했을 때 더 높게 나타났다. Arteima 섭식단계 실험에서, 부화 30일째 넙치자어의 성장은 P. nana (C4-adult) 단독 공급실험구와 혼합공급(P. nana+Artemia) 실험구가 다른 실험구에 비해 높게 나타났으나 넙치 자어의 생존은 모든 실험구가 유의적인 차이를 보이지 않았다. P. nana nauplius와 성체(C4-adult)의 n-3 HUFA 함량은 각각 4.0%와 5.4%였다. 결과적으로, 기수산 요각류 P. nana는 해수어 종묘생산을 위한 초기생먹이로 효과적인 것으로 판단된다.

Keywords

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Fig. 1. Growth (A) and survival (B) of Paralichthys olivaceus larvae fed different diets (C, copepod 100%; CR, copepod 50% + rotifer 50%; R, rotifer 100%) on 12 DAH (days after hatching). Different letters indicate a significant difference (P<0.05).

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Fig. 2. Growth of Paralichthys olivaceus larvae fed different diets on 30 DAH (days after hatching) [C, copepod 100%; CA, copepod 50% + Artemia nauplius 50%; A, Artemia nauplius 100%; CA5, fed C from 14 DAH to 18 DAH and then A from 19 DAH] from 14 DAH to 30 DAH at Artemia nauplius feeding stage. Different letters indicate a significant difference (P<0.05).

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Fig. 3. n-3 HUFA contents (% dry weight) of food organisms fed to Paralichthys olivaceus larvae. Rotifer and Artemia were enriched with A1 Super Selco®.Copepod nauplii and adults were enriched with Tetraselmis suecica.

Table 1. Fatty acids composition (% of total fatty acids) of food organisms fed to the flounder Paralichthys olivaceus larvae. Rotifer and Artemia were enriched with A1 Super Selco. Copepod nauplii and adults were cultured with Tetraselmis suecica*

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Table 2. Fatty acids composition (% of total fatty acids) of Paralichthys olivaceus fed different diets on twelve days after hatching*

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Table 3. Fatty acids composition (% of total fatty acids) of Paralichthys olivaceus fed different diets on 30 DAH*

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