Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Standardization for Analysis Method of Total Polyphenol in Complex of Picao Preto

피카오프레토 등 복합물 중 총 폴리페놀 분석법 표준화

  • Hu, Soojung (Nutrition and Functional Food Research Team, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation) ;
  • Kim, Ji-An (Nutrition and Functional Food Research Team, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation) ;
  • Moon, Myung-Hee (Nutrition and Functional Food Research Team, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation) ;
  • Lee, Sung-Hye (Nutrition and Functional Food Research Team, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation) ;
  • Yoon, Hae-Seong (Nutrition and Functional Food Research Team, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation) ;
  • Hong, Jin-Hwan (Nutrition and Functional Food Research Team, Food Safety Evaluation Department, National Institute of Food and Drug Safety Evaluation)
  • 허수정 (식품의약품안전평가원 식품위해평가부 영양기능연구팀) ;
  • 김지안 (식품의약품안전평가원 식품위해평가부 영양기능연구팀) ;
  • 문명희 (식품의약품안전평가원 식품위해평가부 영양기능연구팀) ;
  • 이성혜 (식품의약품안전평가원 식품위해평가부 영양기능연구팀) ;
  • 윤혜성 (식품의약품안전평가원 식품위해평가부 영양기능연구팀) ;
  • 홍진환 (식품의약품안전평가원 식품위해평가부 영양기능연구팀)
  • Received : 2017.10.16
  • Accepted : 2018.01.30
  • Published : 2018.02.28
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Abstract

As generic health functional food items have been expanded, this research project has been conducted to prepare a scientific and systematic standardized analytical method of relevant food item and examine the suitability of the method for health/functional foods on sale. Total polyphenol was necessary for development and verification of standardized analytical method. The method exhibited high linearity in the tannic acid calibration curve ($r^2$ > 0.999) over concentrations of $5-50{\mu}g/mL$. The limits of detection and quantitation for tannic acid were $5{\mu}g/mL$ and $15{\mu}g/mL$, respectively, while tannic acid recovery was 102.3-112.4% with standard deviations of 0.8-3.2%. To verify the accuracy of the analytical method, the labeled amounts of purchased health functional foods were monitored. The recovery for tannic acid was 105.6% of the labeled amounts. Thus, the new method was suitable for all cases.

피카오프레토 등 복합물의 개별인정 시험법인 총 폴리페놀 시험법에서 Folin-phenol 시약 선정, 발색시약인 35% 탄산나트륨의 반응시간 및 온도, 시료량 등을 비교 실험하여 총 폴리페놀에 대한 표준화된 시험법을 확립하였다. 총 폴리페놀의 표준화된 시험법의 밸리데이션을 수행한 결과 회수율은 102.3~112.4%, 정밀도의 표준편차는 0.81~3.18%였으며 검량선의 직선성은 결정계수($r^2$)가 0.999이상을 나타냈고 검출한계는 $5{\mu}g/mL$, 정량한계는 $15{\mu}g/mL$이었다. 유통제품에 대해 표준화된 총 폴리페놀 시험법의 적용성 검토를 위해 1건의 검체를 수거하여 분석한 결과, 총 폴리페놀 표시 함량 대비 105.6%로 적합하였다.

Keywords

References

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