Journal of the korean academy of Pediatric Dentistry (대한소아치과학회지)

Korean Academy of Pediatric Dentistry (대한소아치과학회)
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Study of Bacteria Associated with Dental Caries Using a 3 Tone Disclosing Agent

세가지 색상차이를 보이는 착색제를 이용한 치아 우식 관련 균에 관한 연구

  • Lee, Jeongeun (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University) ;
  • Park, Howon (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University) ;
  • Lee, Juhyun (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University) ;
  • Seo, Hyunwoo (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University) ;
  • Lee, Siyoung (Department of Oral Microbiology, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University)
  • 이정은 (강릉원주대학교 치과대학 소아청소년치과학교실 및 구강과학연구소) ;
  • 박호원 (강릉원주대학교 치과대학 소아청소년치과학교실 및 구강과학연구소) ;
  • 이주현 (강릉원주대학교 치과대학 소아청소년치과학교실 및 구강과학연구소) ;
  • 서현우 (강릉원주대학교 치과대학 소아청소년치과학교실 및 구강과학연구소) ;
  • 이시영 (강릉원주대학교 치과대학 미생물학교실 및 구강과학연구소)
  • Received : 2017.07.07
  • Accepted : 2017.08.31
  • Published : 2018.02.28
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Abstract

The aim of this study was to determine the efficacy of a 3 tone plaque disclosing gel in assessing the risk of caries related to the population of Streptococcus mutans, Streptococcus sobrinus, and Lactobacillus spp. quantified using a quantitative real-time polymerase chain reaction (qRT-PCR). 15 healthy children of ages 9 - 12 years were randomly examined. The 3 tone plaque disclosing gel was applied on teeth surfaces, which changed the color to pink or red, blue or purple and light blue. Plaque was divided into 3 groups based on staining. Genomic DNA from each sample was subjected to a qRT-PCR assay for quantitative detection of target bacteria. The Kruskal-Wallis test was conducted for correlation between the color of plaque and the number of bacterial species. The levels of S. mutans, S. sobrinus, and Lactobacillus spp. were significantly different in the plaque samples of the 3 groups (p < 0.05). The proportion of S. sobrinus to S. mutans showed correlation to the color of plaque. The different color-dyed plaque was related to the number of acidogenic bacteria. The 3 tone plaque disclosing gel could be used as one of the indicators to assess the clinical risk of caries associated with the population of S. mutans, S. sobrinus, and Lactobacillus spp.

본 연구는 치태의 성숙도에 따라 치태를 서로 다른 색상으로 염색하는 GC Tri Plaque ID $Gel^{TM}$(GC corporation, Tokyo, Japan)을 이용하여 치아 우식 위험도를 평가하고자 하였다. 치아 우식의 발생 및 진행과 연관된 균인 Streptococcus mutans, Streptococcus sobrinus, Lactobacillus spp.의 수를 Quantitative real-time polymerase chain reaction (qRT-PCR)로 측정하여 치아 우식 위험도를 보았다. 본 실험은 강릉원주대학교 치과병원 임상시험 심사위원회의 심의를 받고 진행하였다. 강릉원주대학교 치과병원 소아치과에 내원한 전신질환이 없는 건강한 9 - 12세의 초등학생 15명의 치면을 착색제로 염색하였다. 치태의 성숙도에 따라 서로 다른 세가지 색상으로 염색되었으며 색상별로 3개의 실험군인 I군(pink/red), II군(blue/purple), III군(light blue)으로 나누었다. 3개의 실험군에서 각각 DNA를 추출한 후, qRT-PCR을 이용하여 S. mutans, S. sobrinus, Lactobacillus spp.의 수를 측정하였다. 3개의 실험군 사이에 S. mutans, S. sobrinus와 Lactobacillus spp. 균 수의 유의한 차이가 관찰되었으며 3종류의 균 모두 III군에서 가장 많이 관찰되었다(p < 0.05). GC Tri Plaque ID $Gel^{TM}$는 기존 착색제와는 달리 치태의 성숙도에 따라 서로 다른 세가지 색상으로 염색되며, 치태 염색 색상의 차이는 치아 우식 관련 균 수의 차이를 보여주었다. GC Tri Plaque ID $Gel^{TM}$이 치아 우식 위험도를 평가하는 하나의 지표로서 사용될 수 있는 가능성을 확인하였다.

Keywords

References

  1. Marsh PD : Dental plaque: biological significance of a biofilm and community life-style. J Clin Periodontol , 32:7-15, 2005. https://doi.org/10.1111/j.1600-051X.2005.00790.x
  2. Hojo K, Nagaoka S, Ohshima T, Maeda N : Bacterial interactions in dental biofilm development. J Dent Res , 88:982-990, 2009. https://doi.org/10.1177/0022034509346811
  3. Socransky SS, Gibbons RJ, Dale AC, et al . : The microbiota of the gingival crevice area of man. I. Total microscopic and viable counts and counts of specific organisms. Arch Oral Biol , 8:275-280, 1963. https://doi.org/10.1016/0003-9969(63)90019-0
  4. Klock B, Krasse B : A comparison between different methods for prediction of caries activity. Eur J Oral Sci , 87:129-139, 1979. https://doi.org/10.1111/j.1600-0722.1979.tb00664.x
  5. KOHler B, Pettersson BM, Bratthall D : Streptococcus mutans in plaque and saliva and the development of caries. Eur J Oral Sci , 89:19-25, 1981. https://doi.org/10.1111/j.1600-0722.1981.tb01273.x
  6. Loesche WJ : Role of Streptococcus mutans in human dental decay. Microbiol Rev , 50:353-380, 1986.
  7. Fujiwara T, Sasada E, Mima N, Ooshima T : Caries prevalence and salivary mutans streptococci in 0-2-year-old children of Japan. Community Dent Oral Epidemiol , 19:151-154, 1991. https://doi.org/10.1111/j.1600-0528.1991.tb00131.x
  8. Choi EJ, Lee SH, Kim YJ : Quantitative real-time polymerase chain reaction for Streptococcus mutans and Streptococcus sobrinus in dental plaque samples and its association with early childhood caries. Int J Paediatr Dent , 19:141-147, 2009. https://doi.org/10.1111/j.1365-263X.2008.00942.x
  9. Carlsson J, Grahnen H, Jonsson G : Lactobacilli and Streptococci in the Mouth of Children. Caries Res , 9:333-339, 1975. https://doi.org/10.1159/000260166
  10. Granath L, Cleaton-Jones P, Fatti LP, Grossman ES : Salivary lactobacilli explain dental caries better than salivary mutants streptococci in 4-5-year-old children. Scand J Dent Res , 102:319-323, 1994.
  11. Roeters FJ, van der Hoeven JS, Burgersdijk RC, Schaeken MJ : Lactobacilli, mutants streptococci and dental caries: a longitudinal study in 2-year-old children up to the age of 5 years. Caries Res , 29:272-279, 1995. https://doi.org/10.1159/000262081
  12. Kuribayashi M, Kitasako Y, Matin K, et al . : Intraoral pH measurement of carious lesions with qPCR of cariogenic bacteria to differentiate caries activity. J Dent , 40:222-228, 2012. https://doi.org/10.1016/j.jdent.2011.12.013
  13. Horiuchi M, Washio J, Mayanagi H, Takahashi N : Transient acid-impairment of growth ability of oral Streptococcus, Actinomyces, and Lactobacillus: a possible ecological determinant in dental plaque. Oral Microbiol Immunol, 24:319- 324, 2009. https://doi.org/10.1111/j.1399-302X.2009.00517.x
  14. Simark-Mattsson C, Jonsson R, Emilson CG, Roos K : Final pH affects the interference capacity of naturally occurring oral Lactobacillus strains against mutans streptococci. Arch Oral Biol , 54:602-607, 2009. https://doi.org/10.1016/j.archoralbio.2009.03.005
  15. Jayanthi M, Shilpapriya M, Reddy VN, et al . : Efficacy of three-tone disclosing agent as an adjunct in caries risk assessment. Contemp Clin Dent , 6:358-363, 2015. https://doi.org/10.4103/0976-237X.161887
  16. Yoshida A, Suzuki N, Nakano Y, et al . : Development of a 5' nuclease-based real-time PCR assay for quantitative detection of cariogenic dental pathogens Streptococcus mutans and Streptococcus sobrinus . J Clin Microbiol , 41:4438-4441, 2003. https://doi.org/10.1128/JCM.41.9.4438-4441.2003
  17. Childers NK, Osgood RC, Hsu KL, et al . : Real-time quantitative polymerase chain reaction for enumeration of Streptococcus mutans from oral samples. Eur J Oral Sci , 119: 447-454, 2011. https://doi.org/10.1111/j.1600-0722.2011.00888.x
  18. Morita E, Narikiyo M, Nishimura E, et al . : Molecular analysis of age-related changes of Streptococcus anginosus group and Streptococcus mitis in saliva. Oral Microbiol Immunol , 19:386-389, 2004. https://doi.org/10.1111/j.1399-302x.2004.00173.x
  19. Yano A, Kaneko N, Ida H, et al . : Real-time PCR for quantification of Streptococcus mutans . FEMS Microbiol Lett , 217:23-30, 2002. https://doi.org/10.1111/j.1574-6968.2002.tb11451.x
  20. Byun R, Nadkarni MA, Chhour KL, et al . : Quantitative analysis of diverse Lactobacillus species present in advanced dental caries. J Clin Microbiol , 42:3128-3136, 2004. https://doi.org/10.1128/JCM.42.7.3128-3136.2004
  21. Takahashi N, Nyvad B : The role of bacteria in the caries process: ecological perspectives. J Dent Res , 90:294-303, 2011. https://doi.org/10.1177/0022034510379602
  22. Duchin S, van Houte J : Relationship of Streptococcus mutans and lactobacilli to incipient smooth surface dental caries in man. Arch Oral Biol , 23:779-786, 1978. https://doi.org/10.1016/0003-9969(78)90155-3
  23. Boyar RM, Bowden GH : The microflora associated with the progression of incipient carious lesions of children living in a water-fluoridated area. Caries Res , 19:298-306, 1985. https://doi.org/10.1159/000260859
  24. Milnes AR, Bowden GH : The microflora associated with developing lesions of nursing caries. Caries Res , 19:289-297, 1985. https://doi.org/10.1159/000260858
  25. Igarashi K, Hamada Y, Nishimaki H, et al . : The acidogenic potential of plaque from sound enamel, white spot lesions, and cavities in children. Pediatr Dent , 9:212-215, 1987.
  26. Alaluusua S, Malmivirta R : Early plaque accumulation a sign for caries risk in young children. Community Dent Oral Epidemiol , 22:273-276, 1994. https://doi.org/10.1111/j.1600-0528.1994.tb02049.x
  27. Roeters J, Burgersdijk R, Truin GJ, Hof M : Dental caries and its determinants in 2-to-5-year-old children. ASDC J Dent Child , 62:401-408, 1995.
  28. Wendt LK, Hallonsten AL, Koch G, Birkhed D : Analysis of caries-related factors in infants and toddlers living in Sweden. Acta Odontol Scand , 54:131-137, 1996. https://doi.org/10.3109/00016359609006019