대한소아치과학회지 (Journal of the korean academy of Pediatric Dentistry)

  • 제45권1호
  • /
  • Pages.32-40
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  • 2018
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  • 1226-8496(pISSN)
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  • 2288-3819(eISSN)
대한소아치과학회 (Korean Academy of Pediatric Dentistry)
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세가지 색상차이를 보이는 착색제를 이용한 치아 우식 관련 균에 관한 연구

Study of Bacteria Associated with Dental Caries Using a 3 Tone Disclosing Agent

  • 이정은 (강릉원주대학교 치과대학 소아청소년치과학교실 및 구강과학연구소) ;
  • 박호원 (강릉원주대학교 치과대학 소아청소년치과학교실 및 구강과학연구소) ;
  • 이주현 (강릉원주대학교 치과대학 소아청소년치과학교실 및 구강과학연구소) ;
  • 서현우 (강릉원주대학교 치과대학 소아청소년치과학교실 및 구강과학연구소) ;
  • 이시영 (강릉원주대학교 치과대학 미생물학교실 및 구강과학연구소)
  • Lee, Jeongeun (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University) ;
  • Park, Howon (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University) ;
  • Lee, Juhyun (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University) ;
  • Seo, Hyunwoo (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University) ;
  • Lee, Siyoung (Department of Oral Microbiology, Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University)
  • 투고 : 2017.07.07
  • 심사 : 2017.08.31
  • 발행 : 2018.02.28
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초록

본 연구는 치태의 성숙도에 따라 치태를 서로 다른 색상으로 염색하는 GC Tri Plaque ID $Gel^{TM}$(GC corporation, Tokyo, Japan)을 이용하여 치아 우식 위험도를 평가하고자 하였다. 치아 우식의 발생 및 진행과 연관된 균인 Streptococcus mutans, Streptococcus sobrinus, Lactobacillus spp.의 수를 Quantitative real-time polymerase chain reaction (qRT-PCR)로 측정하여 치아 우식 위험도를 보았다. 본 실험은 강릉원주대학교 치과병원 임상시험 심사위원회의 심의를 받고 진행하였다. 강릉원주대학교 치과병원 소아치과에 내원한 전신질환이 없는 건강한 9 - 12세의 초등학생 15명의 치면을 착색제로 염색하였다. 치태의 성숙도에 따라 서로 다른 세가지 색상으로 염색되었으며 색상별로 3개의 실험군인 I군(pink/red), II군(blue/purple), III군(light blue)으로 나누었다. 3개의 실험군에서 각각 DNA를 추출한 후, qRT-PCR을 이용하여 S. mutans, S. sobrinus, Lactobacillus spp.의 수를 측정하였다. 3개의 실험군 사이에 S. mutans, S. sobrinus와 Lactobacillus spp. 균 수의 유의한 차이가 관찰되었으며 3종류의 균 모두 III군에서 가장 많이 관찰되었다(p < 0.05). GC Tri Plaque ID $Gel^{TM}$는 기존 착색제와는 달리 치태의 성숙도에 따라 서로 다른 세가지 색상으로 염색되며, 치태 염색 색상의 차이는 치아 우식 관련 균 수의 차이를 보여주었다. GC Tri Plaque ID $Gel^{TM}$이 치아 우식 위험도를 평가하는 하나의 지표로서 사용될 수 있는 가능성을 확인하였다.

The aim of this study was to determine the efficacy of a 3 tone plaque disclosing gel in assessing the risk of caries related to the population of Streptococcus mutans, Streptococcus sobrinus, and Lactobacillus spp. quantified using a quantitative real-time polymerase chain reaction (qRT-PCR). 15 healthy children of ages 9 - 12 years were randomly examined. The 3 tone plaque disclosing gel was applied on teeth surfaces, which changed the color to pink or red, blue or purple and light blue. Plaque was divided into 3 groups based on staining. Genomic DNA from each sample was subjected to a qRT-PCR assay for quantitative detection of target bacteria. The Kruskal-Wallis test was conducted for correlation between the color of plaque and the number of bacterial species. The levels of S. mutans, S. sobrinus, and Lactobacillus spp. were significantly different in the plaque samples of the 3 groups (p < 0.05). The proportion of S. sobrinus to S. mutans showed correlation to the color of plaque. The different color-dyed plaque was related to the number of acidogenic bacteria. The 3 tone plaque disclosing gel could be used as one of the indicators to assess the clinical risk of caries associated with the population of S. mutans, S. sobrinus, and Lactobacillus spp.

키워드

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