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넙치(Paralichthys olivaceus) 근육 채취 방법에 따른 Kudoa septempunctata 진단 효율 비교

Evaluation of a Non-destructive Diagnostic Test for Kudoa septempunctata in Farmed Olive Flounder Paralichthys olivaceus

  • 송준영 (국립수산과학원 남동해수산연구소) ;
  • 정승희 (국립수산과학원 병리연구과) ;
  • 최혜승 (국립수산과학원 남해수산연구소)
  • 투고 : 2017.10.23
  • 심사 : 2017.12.01
  • 발행 : 2018.02.28

초록

Kudoa septempunctata, a myxosporean parasite that infects olive flounder Paralichthys olivaceus is known to cause Kudoa food poisoning. Entire trunk muscle (ETM) is used for diagnosis of the parasite in fish and this method demands sacrifice of the host, causing a loss of commercial value. We developed a non-destructive method that uses a plastic syringe-style implanter to draw the sample, called the part-point muscle (PPM) sampling technique. We validated the PPM method in fish infected with K. septempunctata at the level detectable by the ETM method. We confirmed that the PPM method is equally sensitive in comparison to the ETM method for diagnosing K. septempunctata spores in olive flounder muscle. Our study also confirmed that the parasite is uniformly distributed in the dorsal muscle of infected fish. Over a period of 1 month, we observed no mortality of the host fish used for sampling by the PPM method. Thus, our studies demonstrate that the PPM sampling technique is an efficient, non-destructive method for diagnosing K. septempunctata in olive flounder.

키워드

참고문헌

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