Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Development of a Layered Nanocomposite Containing α-pinene to Treat Scuticociliatosis Caused by Miamiensis avidus

알파-피넨(alpha-pinene)이 함유된 스카티카증 치료용 나노복합체 개발 및 Miamiensis avidus에 대한 살충 효과

  • Jung, Chul-Yeon (Nanomaterials Laboratory, Nanospace Co. Ltd.) ;
  • Lee, Sukkyoung (Department of Marine Life Sciences, Jeju National University) ;
  • Yoon, Joo-Young (Nanomaterials Laboratory, Nanospace Co. Ltd.) ;
  • Han, Yang-Su (Nanomaterials Laboratory, Nanospace Co. Ltd.) ;
  • Lee, Jehee (Department of Marine Life Sciences, Jeju National University)
  • Received : 2017.09.07
  • Accepted : 2018.01.15
  • Published : 2018.02.28
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Abstract

Alpha-pinene is an organic compound that possesses antibiotic and anti-parasitic activities. In this study, we developed a layered nanocomposite to combat against Miamiensis avidus which causes scuticociliatosis in the olive flounder Paralichthys olivaceus. We used a solid-solid reaction to develop the layered nanocomposite, incorporating-pinene ($C_{10}H_{16}$) into organically modified montmorillonite. We used cetyltrimethylammonium cations as the interlayered modifier for the adsorption of hydrophobic pinene molecules. The X-ray diffraction patterns of the nanocomposite structure showed that the basal spacing increased from $9.6{\AA}$ to $30.4{\AA}$. Interestingly, the fraction of ${\alpha}$-pinene released remained constant for a long period of time (228 h) due to the layered nature of the nanocomposite. Additionally, optical microscopic images of the treated scuticociliatids revealed that their cells were lysed, and this effect increased with the increasing concertration of ${\alpha}$-pinene. Histopathological assessment of ${\alpha}$-pinene nanocomposite-treated olive flounder gills revealed no significant morphological changes, even at the highest concentration of the ${\alpha}$-pinene. The nanocomposite has several advantages, including easy handling, high solubility, low toxicity, and the easy formulation of granules or powder, which improve the pesticidal activity of ${\alpha}$-pinene. Collectively, our results suggest that ${\alpha}$-pinene nanocomposite may be a useful treatment against scuticociliatosis.

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References

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