Journal of the Korea Convergence Society (한국융합학회논문지)

Korea Convergence Society (한국융합학회)
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Convergence Study of Antimicrobial Resistance of Escherichia coli Isolated from Cheonho Reservoir in Cheonan

천안 천호지에서 분리한 대장균의 항생제 내성에 관한 융합연구

  • Lee, Jin Kyung (Industry Academic Cooperation Foundation, Dankook University) ;
  • Lee, Young ki (Department of Biomedical Laboratory Science, Dankook University) ;
  • Yuk, Young Sam (Department of Biomedical Laboratory Science, Dankook University) ;
  • Kim, Ga-Yeon (Department of Dental Hygiene, Dankook University)
  • 이진경 (단국대학교 산학협력단) ;
  • 이영기 (단국대학교 임상병리학과) ;
  • 육영삼 (단국대학교 임상병리학과) ;
  • 김가연 (단국대학교 치위생학과)
  • Received : 2017.09.14
  • Accepted : 2017.11.20
  • Published : 2017.11.28
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Abstract

This study was to investigate the sanitary microbiological aspects of Cheonho Reservoir and its antibiotic resistance in the natural environment to contribute to the public healthcare improvement. Groups of coliforms, were counted at three sites of the Cheonho Reservoir in July and September 2013, and the isolates were identified according to the water pollution process test standards. Antibiotic resistance was evaluated using the Korea National Institute of Health's standard antibiotic susceptibility test. The average coliform counts in Cheonho Reservoir were $2.0{\times}10^3CFU/mL$, 41% of which were identified as Escherichia coli(E. coli). Antibiotic resistance rate in E. coli isolated from Cheonho reservoir was highest in Ampicillin 31.3%, Ticarcillin 25.0%, Cefaxolin and Cefoxitin 18.8% respectively. Convergence multiple resistance patterns, 38,5% presented resistance to 4 durgs, 6 drugs resistance were 7.7%. The more than 2 drugs resistance were 92.3%. Cheonho Reservoir is a public park that accessible to the citizens, further convergence studies are needed to develop sanitary microbiological management practices and study antibiotic resistance of the reservoir.

본 연구는 천안소재 천호저수지의 위생미생물학적 수준과 자연환경에서의 항생제 내성 분포를 파악하여 국민보건 향상에 이바지 하고자 하였다. 2013년 7월과 9월 3차에 걸쳐 위생지표세균인 대장균군수를 측정하고 수질오염공정시험기준에 따라 분리 동정하였으며 국립보건원 항생제 감수성 표준시험법을 사용하여 항생제 내성 시험을 하였다. 천호저수지의 평균 대장균군수는 $2.0{\times}10^3CFU/mL$이었으며 그 중 41%가 대장균으로 동정되었다. 대장균의 항생제 내성은 Ampicillin 31.3%로 가장 높았으며 Ticarcillin 25.0%, Cefazolin 및 Cefoxitin 이 18.8% 이었다. 융합다제내성은 4제내성이 38.5%, 6제 내성은 7.7%, 2제이상은 내성균의 92.3%를 차지하였다. 천호저수지는 시민들이 이용하는 공원으로 보건위생학적 수질관리를 위해 주기적인 위생미생물학적 연구와 항생제 내성에 관한 융합적인 관리가 필요하다.

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