The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
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Effects of Hyeolbuchugeo-tang on Osteoclast Differentiation and Bone Resorption

혈부축어탕이 파골세포 분화 및 골흡수에 미치는 영향

  • Jang, Sae-Byul (Dept. of Oriental Gynecology, College of Korean Medicine, Dae-Jeon University Graduate School) ;
  • Yoo, Dong-Youl (Dept. of Oriental Gynecology, College of Korean Medicine, Dae-Jeon University Graduate School) ;
  • Yoo, Jeong-Eun (Dept. of Oriental Gynecology, College of Korean Medicine, Dae-Jeon University Graduate School)
  • 장새별 (대전대학교 한의과대학 부인과교실) ;
  • 유동열 (대전대학교 한의과대학 부인과교실) ;
  • 유정은 (대전대학교 한의과대학 부인과교실)
  • Received : 2017.10.24
  • Accepted : 2017.11.17
  • Published : 2017.11.24
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Abstract

Objectives: This study was conducted to evaluate the effects of Hyeolbuchugeo-tang (HBC) on Osteoporosis. Methods: We induced RAW 264.7 cells to differentiate to Osteoclasts by RANKL and treated RANKL-induced RAW 264.7 cells with HBC (0, 150, 350, $700{\mu}g/ml$). To measure osteoclast differentiation and activation, we counted TRAP (+) MNCs and measured mRNA expressions of its related genes (TRAP, MMP-9, cathepsin K, NFATc1, c-Fos, MITF, iNOS, COX-2, TNF-${\alpha}$) by RT-PCR. To assess bone resorption, the Bone pit formation were examined under a microscope. Results: HBC decreased TRAP (+) MNCs and inhibited mRNA expressions of TRAP, MMP-9, cathepsin K, NFATc1, c-Fos, MITF in osteoclast. And HBC inhibited Bone pit formation. Conclusions: HBC inhibited osteoclast differentiation and activation and bone resorption. Taken together, these results indicate that HBC might have potentials for prevention and treatment of Osteoporosis.

Keywords

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