The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology (한방안이비인후피부과학회지)

The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology (대한한방안이비인후피부과학회)
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A Study of Melanin Synthesis Pathway of the Ethanol extract of Eclipta prostrata In Vitro Study

한련초 에탄올 추출물의 멜라닌 합성 경로에 대한 연구

  • Park, In-Hae (Department of Ophthalmology, Otolaryngology and Dermatology of Korean Medicine, College of Korean Medicine, Wonkwang University) ;
  • Hong, Seok-Hun (Department of Ophthalmology, Otolaryngology and Dermatology of Korean Medicine, College of Korean Medicine, Wonkwang University) ;
  • Woo, Won-Hong (Department of Herbal Resources, Professional Graduate School of Korean Medicine, Wonkwang University) ;
  • Mun, Yeun-Ja (Department of Herbal Resources, Professional Graduate School of Korean Medicine, Wonkwang University) ;
  • Cha, Su-Bin (Department of Herbal Resources, Professional Graduate School of Korean Medicine, Wonkwang University)
  • 박인해 (원광대학교 한의과대학 안이비인후피부과학교실) ;
  • 홍석훈 (원광대학교 한의과대학 안이비인후피부과학교실) ;
  • 우원홍 (원광대학교 한의과대학 해부학교실) ;
  • 문연자 (원광대학교 한의과대학 해부학교실) ;
  • 차수빈 (원광대학교 한의과대학 해부학교실)
  • Received : 2017.10.02
  • Accepted : 2017.11.17
  • Published : 2017.11.25
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Abstract

Objectives : This Study was conducted to investigate the melanin synthesis effect of Eclipta prostrata and to determine the relationship between melanin synthesis effect of Eclipta prostrata and cAMP/PKA melanin synthesis pathway. Methods : We measured melanin contents, tyrosinase activity, expression of TRPs, p-CREB in B16F10 cells cultured with Eclipta prostrata ethanol extracts(EEP). And after treatment with H89 and dibutyryl cAMP, which inhibit or promote the activation of PKA, we observed changes in melanin synthesis and tyrosinase activity stimulated by EEP. Results : EEP increased melanin synthesis by promoting the expression of tyrosinase and TRP-1. It also promoted expression of p-CREB. H89 suppresses melanogenic effect and expression of tyrosinase, TRP-1 in B16F10 stimulated by EEP. Dibutyryl cAMP promotes melanogenic effect of EEP. Conclusions : The results of this study suggest that melanin synthesis effect of EEP is related to induction of tyrosinase and TRP-1 expression through the cAMP/PKA pathway.

Keywords

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