DOI QR코드

DOI QR Code

Effects of Lactoferrin and Transferrin on the Growth of Lactococcus lactis subsp. cremoris FC

Lactococcus lactis subsp. cremoris FC에 대한 Lactoferrin과 Transferrin의 생장촉진효과

  • Kim, Woan-Sub (Dept. of Animal Life and Environmental Science, Hankyong National University)
  • 김완섭 (한경대학교 동물생명환경과학과)
  • Received : 2017.09.13
  • Accepted : 2017.09.18
  • Published : 2017.09.30

Abstract

Recent studies have reported that certain lactic acid bacteria and their metabolites, such as exopolysaccharides (EPSs), have immunological effects and can modulate the immune system following oral administration. Lactococcus lactis subsp. cremoris FC is a lactic acid bacteria isolated from fermented milk from Caucasians and has been shown to produce EPSs. In this study, the effects of lactoferrins (apo-lactoferrin, holo-lactoferrin, and native-lactoferrin) and transferrins (apo-transferrin and holo-lactoferrin) on the growth of L. lactis subsp. cremoris FC were examined. The addition of lactoferrins and transferrins to L. lactis subsp. cremoris FC cultures was found to be effective at concentrations of 0.5 or 1 mg/mL.

Lactococcus lactis subsp. cremoris FC는 카스피해 요구르트의 명칭으로 강한 점성을 가지는 것을 특징으로 하는 코카커스 지방 유래의 발효유로부터 분리된 균주로 있다. 본 연구는 Lc. lactis subsp. cremoris FC의 생장에 대한 lactoferrin group(native-lactoferrin, apo-lactoferrin, 그리고 holo-lactoferrin)과 transferrin (apo-transferrin과 holo-transferrin)의 첨가가 생장에 미치는 영향을 알아보기 위하여 수행하였다. Lc. lactis subsp. cremoris FC의 생장에 lactoferrin과 transferrin의 첨가효과는 0.5 또는 1 mg/mL의 농도로 첨가하는 것이 생장 촉진에 좋은 효과를 나타내는 것으로 조사되었다.

Keywords

References

  1. Bjorksten, B., Naaber, P., Sepp, E. and Mikelsaar, M. 1999. The intestinal microflora in allergic Estonian and Swedish 2-year-old children. Clin. Exp. Allergy 29:342-346. https://doi.org/10.1046/j.1365-2222.1999.00560.x
  2. Bjorksten, B., Sepp, E., Julge, K., Voor, T. and Mikelsaar, M. 2001. Allergy development and the intestinal microflora during the first year of life. J. Allergy Clin. Immunol. 108:516-520. https://doi.org/10.1067/mai.2001.118130
  3. Chesler, D. A. and Reiss, C. S. 2002. The role of INF-gamma in immune responses to viral infections of the central nervous system. Cytokine Growth Factor Rev. 13:441-454. https://doi.org/10.1016/S1359-6101(02)00044-8
  4. FAO/WHO. 2002. Guidelines for the evaluation of probiotics in food. Report of a Joint FAO/WHO Working Group on Drafting Guidelines for the Evaluation of Probiotics in Food. London, Ontario, Canada.
  5. Fuller, R. 1989. Probiotics in man and animals. J. Appl. Bacteriol. 66:365-378. https://doi.org/10.1111/j.1365-2672.1989.tb05105.x
  6. Furukawa, N., Iiyama, R., Matuoka, A., Takahashi, T. and Yamanaka, Y. 1992. Reduction of fermentation time of yoghurt using Lactococcus lactis subsp. cremoris. Jpn. Poult. Sci. 63:662-664.
  7. Gibson, G. R. and Roberfroid, M. B. 1995. Dietary modulation of the human colonic microbiota: Introducing the concept of prebiotics. J. Nutr. 125:1401-1412.
  8. Gionchetti, P., Rizzello, F., Venturi, A. and Campieri, M. 2000. Probiotics in infective diarrhea and inflammatory bowel disease. J. Gastroenterol Hepatol. 15:489-493. https://doi.org/10.1046/j.1440-1746.2000.02162.x
  9. Gionchetti, P., Rizzello, F., Helwig, U., Ventura, A., Lammers, K. M. and Brigidi, P. 2003. Prophylaxis of pouchitis onset with probiotic therapy: a double-blind, placebo-controlled trial. Gastroeterology. 124:1202-1209. https://doi.org/10.1016/S0016-5085(03)00171-9
  10. Gotoh, Y., Suzuki, S., Amako, M., Kitamura, S. and Toda, T. 2017. Effects of orally administered exopolysaccharides by Lactococcus lactis subsp. cremoris FC on a mouse model of dermatitis induced by repeated exposure to 2,4,6-trinitro-1-chlorobenzene. J. Funct. Foods. 35:43-50. https://doi.org/10.1016/j.jff.2017.04.045
  11. Groves, M. I. 1960. The isolation of a red protein from milk. J. Am. Chem. Soc. 82:3345-3350. https://doi.org/10.1021/ja01498a029
  12. Helin, T., Haahtela, S. and Haahtela, T. 2002. No effect of oral treatment with an intestinal bacterial strain, Lactobacillus rhamnosus(ATCC 53103), on birch-pollen allergy: a placebo-controlled double-blind study. Allergy 57:243-246. https://doi.org/10.1034/j.1398-9995.2002.1s3299.x
  13. Huebner, J., Wehling, R. L. and Hutkins, R. W. 2007. Functional activity of commercial prebiotics. Int. Dairy J. 17:770-775. https://doi.org/10.1016/j.idairyj.2006.10.006
  14. Ikeda, H., Old, L. J. and Schreiber, R. D. 2002. The roles INF gamma in protection against tumor development and cancer immunoediting. Cytokine Growth Factor Rev. 13:95-109. https://doi.org/10.1016/S1359-6101(01)00038-7
  15. Isolauri, E., Juntunen, M., Rautanen, T., Sillanaukee, P. and Koivula, T. 1991. A human Lactobacillus strain(Lactobacillus casei sp. strain GG) promotes recovery from acute diarrhea in children. Pediatrics 88:90-97.
  16. Kaplan, H. and Hutkins, R. W. 2003. Metabolism of fructooligosaccharides by Lactobacillus paracasei 1195. Appl. Environ. Microbiol. 69:2217-2222. https://doi.org/10.1128/AEM.69.4.2217-2222.2003
  17. Kim, W. S., Ohashi, M., Tanaka, T., Kumura, H., Kim, G. Y., Kwon, I. K., Goh, J. S. and Shimazaki, K. 2004. Growthpromoting effects of lactoferrin on L. acidophikus and Bifidobacterium spp. Biometals 17:279-283. https://doi.org/10.1023/B:BIOM.0000027705.57430.f1
  18. Kimoto, H., Kurisaki, J., Tsuji, N. M., Ohmomo, S. and Okamoto, T. 1999. Lactococci as probiotic strain: adhesion to human enterocyte-like Caco-2 cells and tolerance to low pH and bile. Lett. Appl. Microbiol. 29: 313-316. https://doi.org/10.1046/j.1365-2672.1999.00627.x
  19. Kimoto, H., Nomura, M., Kobayashi, M., Mizumachi, K. and Okamoto, T. 2003. Survival of lactococci during passage through mouse digestive tract. Can. J. Microbiol. 49:707-711. https://doi.org/10.1139/w03-092
  20. Kitazawa, H., Toba, T., Itoh, T., Kumano, N., Adachi, S. and Yamaguchi, T. 1991. Antitumoral activity of slime-forming, encapsulated Lactococcus lactis subsp. cremoris isolated from scandinavian ropy sour milk, "viili". Anim. Sci. Technol 62:277-283.
  21. Kosaka, A., Yan, H., Ohashi, S., Gotoh, Y., Sato, A., Tsutsui, H., Kaisho, T., Toda, T. and Tsuji, N. M. 2012. Lactococcus lactis subsp. cremoris FC triggers IFN-${\gamma}$ production from NK and T cells via IL-12 and IL-18. Int. Immunopharmacol. 14:729-733. https://doi.org/10.1016/j.intimp.2012.10.007
  22. Leon, F., Roldan, E., Sanchez, I., Camarero, C., Bootello, A. and Ray, G. 2003. Humal small-intestinal epithelium contains functional natural killer lymphocytes. Gastroenterology 125:345-356. https://doi.org/10.1016/S0016-5085(03)00886-2
  23. Link-Amster, H., Rochat, F., Saudan, K. Y., Mignot, O. and Aeschlimann, J. M. 1994. Modulation of a specific humoral immue response and changes in intestinal flora mediated through fermented milk intake. FEMS Immunol. Med. Microbiol. 10:55-63. https://doi.org/10.1111/j.1574-695X.1994.tb00011.x
  24. Macura, D. and Townsley, P. M. 1984. Scandinavian ropy milk - identification and characterization of endogenous ropy lactic streptococci and their extracellular excretion. J. Dairy Sci. 67:735-744. https://doi.org/10.3168/jds.S0022-0302(84)81363-6
  25. Majamaa, H., Isolauri, E., Saxelin, M. and Vesikari, T. 1995. Lactic acid bacteria in the treatment of acute rotavirus gastroenteritis. J. Pediatr. Gastroenterol. Nutr. 20:333-338. https://doi.org/10.1097/00005176-199504000-00012
  26. Maruo, T., Gotoh, Y., Nishimura, H., Ohashi, S., Toda, T. and Takahashi, K. 2012. Oral administration of milk fermented with Lactococcus lactis subsp. cremoris FC protects mice against influenza virus infection. Lett. Appl. Microbiol. 55:135-140. https://doi.org/10.1111/j.1472-765X.2012.03270.x
  27. Masson, P. L., Heremans, J. F., Prignot, J. J. and Wauters, G. 1966. Immunohistochemical localization and bactrtioststic properties of an iron-binding protein from bronchial mucus. Thorax., 21:538-544. https://doi.org/10.1136/thx.21.6.538
  28. Metchnikoff, E. 1907. The prolongation of life. GP Putman, New York.
  29. Nakajima, H., Suzuki, Y., Kaizu, H. and Hirota, T. 1992. Cholesterol lowering activity of ropy fermented milk. J. Food Sci. 57:1327-1329. https://doi.org/10.1111/j.1365-2621.1992.tb06848.x
  30. Niedzielin, K., Kordecki, H. and Birkenfeld, B. 2001. A controlled, double-blind, randomized study on the efficacy of Lactobacillus plantarum 299V in patients with irritable bowel syndrome. Eur. J. Gastroenterol. Hepatol. 13:1143-1147. https://doi.org/10.1097/00042737-200110000-00004
  31. Nishitani, Y. and Mizuno, M. 2010. Anti-inflammatory activities of Lactococcus lactis subsp. cremoris FC in in vitro and in vivo gut inflammation models. Biosci. Microflora 29:169-178. https://doi.org/10.12938/bifidus.29.169
  32. Shimazaki, K. 2000. Lactoferrin: A marvelous protein in milk? Anim. Sci. 71:329-347.
  33. Shimazaki, K. and Hosokawa, T. 1991. A tentative method for rapid preparation of iron-saturated lactoferrin by affinity chromatography. Animal Sci. Technol. (Chikusan Gakkai-ho) 62:354-356.
  34. Smit, G., Smit, B. A. and Engels, W. J. 2005. Flavour formation by lactic acid bacteria and biochemical flavour profiling of cheese products. FEMS Microbiol. Rev. 29:591-610. https://doi.org/10.1016/j.fmrre.2005.04.002
  35. Sorensen, M. and Sorensen, S. P. L. 1938. The proteins in whey. Comptes rendus des travaux du laboratoire carlsberg. Ser. Chimique. 23:55-99.
  36. Spanhaak, S., Havenaar, R. and Schaafsma, G. 1998. The effects of consumption of milk fermented by Lactobacillus casei strain Shirota on the intestinal microflora and immune parameters in humans. Eur. J. Clin. Nutr. 52:899-907. https://doi.org/10.1038/sj.ejcn.1600663
  37. Sziksz, E., Vannay, A. and Haczku, A. 2012. Galectin-9: a suppressor of food allergy? Allergy 67:293-295. https://doi.org/10.1111/j.1398-9995.2011.02774.x
  38. Toda, T., Kosaka, H., Terai, M., Mori, H., Benno, Y. and Yamori, Y. 2005. Effects of fermented milk with Lactococcus lactis subsp. cremoris FC on defecation frequency and fecal microflora in healthy elderly volunteers. Nippon Shokuhin Kagaku Kogaku Kaishi 52:243-250. https://doi.org/10.3136/nskkk.52.243
  39. Yang, F., Lum, J. B., McGill, J. R., Moore, C. M., Naylor, S. L., van Braqt, P. H., Baldwin, W. D. and Bowman, B. H. 1984. Human transferrin: cDNA characterization and chromosomal localization. Proc. Natl. Acad. Sci. USA 81:2752-2756. https://doi.org/10.1073/pnas.81.9.2752

Cited by

  1. Effects of Heat-treated Bovine Lactoferrin on the Growth of Lactococcus lactis subsp. cremoris JCM 20076 vol.37, pp.2, 2017, https://doi.org/10.22424/jmsb.2019.37.2.129