한국균학회지 (The Korean Journal of Mycology)

  • 제45권3호
  • /
  • Pages.229-233
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  • 2017
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  • 0253-651X(pISSN)
  • /
  • 2383-5249(eISSN)
한국균학회 (The Korean Society of Mycology)
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철쭉의 뿌리에서 분리된 두 종의 국내 미기록 내생균

Two Novel Species of Endophytic Fungi Isolated from Roots of Rhododendron schlippenbachii in Korea

  • 박혁 (한국교원대학교 생물교육과) ;
  • 엄안흠 (한국교원대학교 생물교육과)
  • Park, Hyeok (Department of Biology Education, Korea National University of Education) ;
  • Eom, Ahn-Heum (Department of Biology Education, Korea National University of Education)
  • 투고 : 2017.08.09
  • 심사 : 2017.08.22
  • 발행 : 2017.09.01
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초록

본 연구에서는 민주지산에 서식하는 철쭉(Rhododendron schlippenbachii)의 뿌리에서 내생균을 분리하였다. 형태적 특성의 확인 및 ribosomal DNA의 internal transcribed spacer(ITS) 지역, large subunit (LSU)지역의 분자적 분석을 통해 국내 미기록종인 Engyodontium album, Phaeosphaeria microscopica 2종을 동정하였다. 연구 과정에서 확인된 미기록종 내생균 2종의 형태적, 분자적 특성을 여기에 기술하였다.

We isolated endophytic fungal strains from roots of Rhododendron schlippenbachii growing in Minjujisan mountain, Korea. The two strains were identified as Engyodontium album and Phaeosphaeria microscopica based on their morphological characteristics and sequence analysis of the internal transcribed spacer (ITS) and large subunit regions of the ribosomal DNA. These two species, E. album and P. microscopica, have not been previously reported in Korea.

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참고문헌

  1. Smith SE, Read DJ. Mycorrhizal symbiosis. 2nd ed. London: Academic Press; 1997.
  2. Carroll G. Fungal endophytes in stems and leaves: from latent pathogen to mutualistic symbiont. Ecology 1988;69:2-9. https://doi.org/10.2307/1943154
  3. Rodriguez RJ, White JF Jr, Arnold AE, Redman RS. Fungal endophytes: diversity and functional roles. New Phytol 2009;182:314-30. https://doi.org/10.1111/j.1469-8137.2009.02773.x
  4. Schardl CL, Craven KD, Speakman S, Stromberg A, Lindstrom A, Yoshida R. A novel test for host-symbiont codivergence indicates ancient origin of fungal endophytes in grasses. Syst Biol 2008;57:483-98. https://doi.org/10.1080/10635150802172184
  5. Eo JK, Lee BH, Eom AH. Diversity of Endophytes Isolated from Thuja koraiensis Nakai in the Korean Peninsula. Kor J Mycol 2016;44:113-7.
  6. Ahlich K, Sieber TN. The profusion of dark septate endophytic fungi in non- ectomycorrhizal fine roots of forest trees and shrubs. New Phytol 1996;132:259-70. https://doi.org/10.1111/j.1469-8137.1996.tb01845.x
  7. Gardes M, Bruns TD. ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rusts. Mol Ecol 1993;2:113-8. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x
  8. Moncalvo JM, Lutzoni FM, Rehner SA, Johnson J, Vilgalys R. Phylogenetic relationships of agaric fungi based on nuclear large subunit ribosomal DNA sequences. Syst Biol 2000;49:278-305. https://doi.org/10.1093/sysbio/49.2.278
  9. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30:2725-9. https://doi.org/10.1093/molbev/mst197
  10. de Hoog GS. Notes on some fungicolous hyphomycetes and their relatives. Persoonia 1978;10:33-81.
  11. Wu H, Yang HY, You XL, Li YH. Diversity of endophytic fungi from roots of Panax ginseng and their saponin yield capacities. SpringerPlus 2013;2:107. https://doi.org/10.1186/2193-1801-2-107
  12. Sanchez Marquez S, Bills GF, Zabalgogeazcoa I. The endophytic mycobiota of the grass Dactylis glomerata. Fungal Divers 2007;27:171-95.
  13. Sanchez Marquez S, Bills GF, Zabalgogeazcoa I. Diversity and structure of the fungal endophytic assemblages from two sympatric coastal grasses. Fungal Divers 2008;33:87-100
  14. Simonovicova A, Godyova M, Kunert J. Engyodontium album, a new species of microscopic fungi for Slovakia and its keratinolytic activity. Biologia (Bratisl) 2004;59:17-8.
  15. Camara MP, Palm ME, van Berkum P, O'Neill NR. Molecular phylogeny of Leptosphaeria and Phaeosphaeria. Mycologia 2002;94:630-40. https://doi.org/10.1080/15572536.2003.11833191
  16. Hasford RM Jr. Effects of wetting period on resistance to leaf spotting of wheat by Leptosphaeria microscopica with conidial stage Phaeoseptoria urvilleana. Phytopathology 1978;68:908-12. https://doi.org/10.1094/Phyto-68-908
  17. Webster J. Graminicolous pyrenomycetes. V. Conidial states of Leptosphaeria michotii, L. microscopica, Pleospora vagans and the perfect state of Dinemasporium graminum. Trans Br Mycol Soc 1955;38:347-65. https://doi.org/10.1016/S0007-1536(55)80038-1