• Korean Journal of Agricultural Science
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• v.12 no.1
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• pp.31-36
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• 1985

Peroxidase banding patterns were obtained by disc electrophoresis of leaf extractions from flowering plants, R. mucronulatum, R. yedoense var. poukhanese, and R. schlippenbachii. A total of 21 peroxidase isoenzymes were resolved from these species, R. mucronulatum, P. yedoense var. poukhanese, and R. schlippenbachii. Five identical peroxidase isoenzymes were resolved in R. yedoense var. poukhanese and R. schlippenbachii. It means that there is a close relationships between two species. The peroxidase banding patterns of all three Rhododendron species were not correlated with each other on percentage total density of each band. The correlation coefficients for peroxidase band densities compared with leaf areas and leaf width/length ratios of Rhododendron plants were not significant at the 5% level. Wide variation was observed in R. yedoense var. poukhanese and R. schlippenbachii compared with R. mucronulatum.

• The Korean Journal of Mycology
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• v.45 no.3
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• pp.229-233
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• 2017

We isolated endophytic fungal strains from roots of Rhododendron schlippenbachii growing in Minjujisan mountain, Korea. The two strains were identified as Engyodontium album and Phaeosphaeria microscopica based on their morphological characteristics and sequence analysis of the internal transcribed spacer (ITS) and large subunit regions of the ribosomal DNA. These two species, E. album and P. microscopica, have not been previously reported in Korea.

• Journal of the Korean Wood Science and Technology
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• v.48 no.2
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• pp.166-180
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• 2020

Rhododendron schlippenbachii have been used as a medicine because of their various biological activities. In this study, R. schlippenbachii ethanol extract was evaluated for the treatment of vitiligo. The R. schlippenbachii ethanol extract did not show any cell cytotoxicity. The effect on mushroom tyrosinase and cellular tyrosinase activities were further assessed. In addition, the determination of melanin content in melanocytes was measured using both the B16 melanoma cells and C57BL/6J Ler-vit/vit mice. Finally, the existence of quercetin in R. schlippenbachii was confirmed by qualitative analysis using HPLC. The results clearly demonstrated the R. schlippenbachii extract enhanced melanogenesis and also increased tyrosinase activity in cultured melanoma cells and C57BL/6J Ler-vit/vit mice. In addition, treatment with R. schlippenbachii extract led to a higher content of melanin and eumelanin in C57BL/6J Ler-vit/vit mice hair than in control (untreated) mice, which demonstrated the therapeutic effect of hair-graying associated with vitiligo. Finally, we confirmed a notable increase in melanocytes in the skin of C57BL/6J Ler-vit/vit mice treated with R. schlippenbachii extract compared with the control. Extracts of R. schlippenbachii was shown to be potent tyrosinase and melanin synthesis activator in B16 melanoma cells. The R. schlippenbachii extract have significantly higher melanin content than the untreated control in C57BL/6J Ler-vit/vit mice hair. The results suggest that R. schlippenbachii extract might be considered as an alternative treatment for improvement of vitiligo.

• Journal of Veterinary Clinics
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• v.26 no.4
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• pp.344-347
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• 2009

Four sheep and five Korean native goats became intoxicated after eating Rhododendron schlippenbachii clippings that a ranchman threw into the animal barn. The clinical signs included loss of appetite, lethargy, nausea, salivation, vomiting, dyspnea, staggering gait, and bradycardia. The animals were treated with atropine and carbo-pulbit. One goat died 8 hours later, and a field postmortem revealed a considerable quantity of rhododendron leaves in the rumen. The other sheep and goats recovered within 3 days after treatment.

• Korean Journal of Agricultural Science
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• v.44 no.1
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• pp.104-113
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• 2017

Anthocyanins contained in Rhododendron schlippenbachii (royal azalea) are expressed in a variety of colors and affect flower colors. R. schlippenbachii flowers of seven colors (white, red group: pink, deep pink, red, purple group: light purple, purple, deep purple) were collected from the garden around KT&G building in the college of agriculture and life science. Seven types of anthocyanins [cyanidin 3-O-diglucoside, cyanidin 3-O-arabinoside-5-O-glucoside, cyanidin 3-O-galactoside, peonidin 3-O-arabinoside-5-O-glucoside, cyanidin 3-O-glucoside, cyanidin 3-O-(6"-O-malonyl) arabinoside, cyanidin 3-O-(6"-O-coumaroyl) glucoside] turned out to be from the cyanidin and peonidin series in R. schlippenbachii flowers. Also, seven types of flavonols [azaleatin 3-O-glucoside, azaleatin 3-O-arabinoside, azaleatin 3-O-rhamnoside, quercetin 3-O-galacatoside, quercetin 3-O-glucoside, quercetin 3-O-arabinoside, quercetin 3-O-rhamnoside] were identified in R. schlippenbachii flowers. Total anthocyanin amounts decreased in R. schlippenbachii flowers in the following order: 'deep pink' (8.07) > 'red' (6.37) > 'pink' (5.35) > 'deep purple' (0.78) > 'purple' (0.43) > 'light purple' ($0.22mg{\cdot}g^{-1}$ dry weight, DW) > 'white' (not detected). Total flavonol amounts decreased in the following order: 'pink' (97.78) > 'deep pink' (63.79) > 'deep purple' (61.98) > 'white' (57.58) > 'light purple' (47.06) > 'purple' (46.76) > 'red' ($7.60mg{\cdot}g^{-1}$ dry weight, DW). This study provided the quantitative and qualitative information for the variation of anthocyanin and flavonol compounds in R. schlippenbachii flowers. Furthermore, this information can contribute to the identification of anthocyanin and flavonol compounds in other Rhododendron flowers.

• Korean Journal of Medicinal Crop Science
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• v.21 no.6
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• pp.439-443
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• 2013

This study was conducted to investigate the antioxidative activity and tyrosinase inhibitory activity of 50% ethanol extract and its fractions from the branch of Rhododendron schlippenbachii. In DPPH radical scavenging ability, butanol and ethyl acetate fractions showed 59.98% and 55.17% of relative activity compared with positive control (ascorbic acid), but the 50% ethanol extract showed relatively low activity. In nitric oxide (NO) scavenging ability, the ethyl acetate and butanol fractions showed 141.80% and 131.55% relative activity compared with ascorbic acid as used for positive control. On the other hand, tyrosinase inhibitory activity of the ethyl acetate and butanol fractions showed about twice higher activity than positive control (arbutin). It means that the ethyl acetate and butanol fractions from the extract of R. schlippenbachii branch has ability for used as effective radical scavenger and tyrosinase inhibitor.

• Journal of Environmental Science International
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• v.20 no.4
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• pp.527-540
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• 2011

The investigation was made about distribution and ecological characteristics of Rhododendron mucronulatum var. mucronulatum, R. dauricum and R. schlippenbachii on Mt. Changbai in China. The Ericaceae plants are known to be highly ornamental and easy to cultivate. The results of investigations are like followings. The plant species diversity was the highest in habitats of R. mucronulatum var. mucronulatum, followed by those of R. schlippenbachii and R. dauricum. R. mucronulatum var. mucronulatum community was found in the northwest exposure areas of 300m in altitude. Corylus heterophylla, Carex humilis var. nana, Atractylodes japonica species also grew in the R. mucronulatum var. mucronulatum community. R. dauricum communities were widely distributed in the west exposure areas of 1226-1860m above sea level. R. schlippenbachii populations were found in areas of under 300m above sea level, which has dry soil. But humidity of air was relatively high in the place. Artemisia japonica, Lespedeza bicolor and Dactylis glomerata were found around the habitats of R. schlippenbachii. Phalanx type of plants were dominant near the habitats of Ericaceae plants as 61%. Further, there appeared higher plant life totalling to 72 taxa comprising 54 species, 16 varieties and 2 forma with 31 families and 59 genuses at the whole survey area. Among these, Compositae plants excellent in resistance to environment was surveyed the most, accounting for 30.0%.

• The Korean Journal of Ecology
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• v.8 no.1
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• pp.53-59
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• 1985

Phenological development, flowering and leafin times of Rhododendron muronulatum and R. schlippenbachii along elevagtion were studied at Mt. Kwanak, 629m high above the sea level, in Seoul. Flowering and leafing time of the former were delayed at the rate of 2.3~3.3 days and those of the latter were of 2.0~3.0 days per 100m ascent. Phenological changes of both plant species were closely correlated with minimum air temperature first, and then soil water content and minimum soil temperature among the climatic factors. Phenological difference caused by altitude and slope direction(southeast-northwest) among the topographic factors was admitted at the high significance level, but the difference by ridgevalley was little.

• Korean Journal of Environment and Ecology
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• v.11 no.4
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• pp.397-406
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• 1998

To investigate the structure and the conservation strategy of natural forest at Taech'ongbong-Hangyeryong Area in Soraksan. 45 plots(20m*20m) set up with random sampling method were surveted. Four groups - Quercus mongolica - Acer pseudosieboldianum community, Avies nephrolepis - Tilia amurensis - Quercus mongolica community, Abies nephrolepis - Taxus cuspidata community, Abies nephrolepis - Qurecusmongolica community - were classified by cluster analysis. High positive correlations were proved between Abies nephrolepis and Betula ermanii; Abies nephrolepis and Pinus pumila; Aies nephrolepis and Rhododendron mucronulatum var. ciliatum; Betula ermanii and Pinus pumila; Betula ermanii and Rhododendron mucronulatum var. ciliatum; Pinus pumila and Rhododendron mucronulatm var. ciliatum; Quercus mongolica and Rhododendron schlippenbachii; Quercus mongolica and Qeigela subsessilis; Euonymus macroptera and Syringa reticulata; Euonymus macroptera and Viburnum wrightii; Acer ukurunduense and Euonymus oxyphyllus, and high nagative correlations were proved between Betula ermanii and Acer pseudosieboldianum; Quercus mongolica and Tripterygium regelii; Rhododendron schlippenbachii and Tripterygium regelii; Carpinus cordata and Acer pseudosieboldianum. Species diversity(H') of investigated area was calculated 2.1352~2.8016.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.11 no.4
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• pp.67-74
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• 2008

This study was carried out to select proper species for early stage replantation in cutting-slope area of the Baekdu Range roadside. In the cutting-slope area and upper forest area of the cutting-slope area, sample plots of 104 were selected and their vegetations and environmental factors were investigated. We found total 151 species in the 104 plots. The species of high frequency of Baekdu Range roadside were found in the order of Lespedeza bicolor, Festuca arundinacea, Eragrostis curvula, Pinus densiflora, Salix hulteni, Lespedeza cuneata, Erigeron annuus, Rubus crataegifolius, Spodiopogon sibiricus, etc. The dominancy of Festuca arundinacea, Eragrostis curvula, Lespedeza bicolor, Pinus densiflora, Salix hulteni, Spodiopogon sibiricus, Miscanthus sinensis, Lespedeza maximowiczii, Acer ginnala were 12.74%, 10.43%, 7.48%, 4.42%, 4.09%, 3.13%, 2.49%, 2.45%, 2.40% in the roadside cutting-slope, respectively. The importance value of Quercus mongolica, Lindera obtusiloba, Rhododendron mucronulatum, Lespedeza maximowiczii, Rhododendron schlippenbachii, Lespedeza bicolor, Quercus serrata, Rhus trichocarpa were 17.32%, 13.82%, 11.60%, 11.31%, 8.76%, 8.04%, 7.65%, 7.10% in shrub layer of the upper forest area, respectively. The species of Carex lanceolata, Spodiopogon sibiricus, Carex siderosticta, Miscanthus sinensis, Artemisia sp., Potentilla fragarioides var. major, Astilbe chinensis var. davidii, Chrysanthemum zawadskii var. latilobum, Aster scaber, Lysimachia clethroides, Patrinia villosa, Lindera obtusiloba, Rhododendron mucronulatum, Rhododendron schlippenbachii, Rhus trichocarpa, Stephanandra incisa, Zanthoxylum schinifolium, Lespedeza sp., Lespedeza cuneata, Amorpha fruticosa, Ligustrum obtusifolium, and Weigela subsessilis, may be more helpful in stabilizing of the cutting-slope area and making harmony with the surrounding forest area.