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수확시기에 따른 까마중 열매의 유용성분 및 생리활성 변화

Changes of useful components and biological activities of Solanum nigrum Linne fruit according to different harvest time

  • 최성규 (순천대학교 한약자원개발학과) ;
  • 윤경원 (순천대학교 한약자원개발학과) ;
  • 김용두 (순천대학교 식품공학과) ;
  • 진성우 ((재)장흥군버섯산업연구원) ;
  • 김경제 ((재)장흥군버섯산업연구원) ;
  • 강경윤 (순천대학교 식품공학과) ;
  • 고영우 ((재)장흥군버섯산업연구원) ;
  • 임승빈 ((재)장흥군버섯산업연구원) ;
  • 정상욱 ((재)장흥군버섯산업연구원) ;
  • 서경순 ((재)장흥군버섯산업연구원)
  • Choi, Seongkyu (Department of Oriental Medicine Resources, Sunchon National University) ;
  • Yun, Kyeong-Won (Department of Oriental Medicine Resources, Sunchon National University) ;
  • Kim, Yong-Doo (Department of Food Science and Technology, Sunchon National University) ;
  • Jin, Seong-Woo (Jangheung research institute for mushroom industry) ;
  • Kim, Kyung-Je (Jangheung research institute for mushroom industry) ;
  • Kang, Kyeong-Yun (Department of Pharmacy, Sunchon National University) ;
  • Koh, Young-Woo (Jangheung research institute for mushroom industry) ;
  • Im, Seung-bin (Jangheung research institute for mushroom industry) ;
  • Jeong, Sang-Wook (Jangheung research institute for mushroom industry) ;
  • Seo, Kyoung-Sun (Jangheung research institute for mushroom industry)
  • 투고 : 2017.09.14
  • 심사 : 2017.10.23
  • 발행 : 2017.10.30

초록

본 연구는 까마중 열매의 수확시기에 따른 성분변화, 항산화효과 및 항염증 효과를 분석하여 최적 수확시기를 구명하였다. 까마중 열매의 유리당은 4가지가 검출되었으며, 유리당 중 fructose는 대체적으로 수확시기가 늦어짐에 따라 증가하였다. 까마중 열매의 주요 유기산은 malic acid와 acetic acid로 나타났으며, 총 유기산 함량은 10월 18일과 10월 25일에 수확한 까마중 열매들에서 높게 나타났다. 총 폴리페놀 함량은 10월 18일에 수확한 까마중 열매, 10월 11일에 수확한 까마중 열매 및 10월 25일에 수확한 까마중열매 순으로 높게 나타났다. 수확시기별 까마중 ethanol 추출물들의 항산화효과와 항염증효과 검정을 위하여 DPPH radical scavenging assay와 ABTS radical scavenging assay를 수행하였으며, 항염증 효과 측정을 위하여 lipopolysaccharide(LPS)로 염증을 유발시킨 RAW 264.7 cells을 NO 저해실험에 사용하였다. 항산화 효과와 항염증 효과는 10월 18일에 수확한 까마중 열매 ethanol 추출물에서 가장 높은 활성을 나타내었다. 10월 18일에 수확한 까마중 열매는 총 폴리페놀 함량, 항산화 효과 및 항염증 효과가 우수하였다. 까마중 열매의 수확시기에 따른 성분분석 및 생리활성 실험 결과, 까마중 열매의 최적 수확시기는 성숙 중기인 10월 18일과 10월 11일로 밝혀졌다.

In this study, we investigated the variation in free sugars, organic acids, amino acids, antioxidant activity and anti-inflammatory effect of Solanum nigrum Linne fruits according to harvest time. Four kinds of free sugars (fructose, glucose, sucrose, maltose) were detected in S. nigrum fruit, and the free sugar contents varied significantly with harvest time. Organic acid content of S. nigrum fruit showed the highest in malic acid and acetic acid, and the highest content of total organic acids was found in S. nigrum fruit harvested on October $18^{th}$ and October $25^{th}$. For the total polyphenol content, S. nigrum fruit harvested on October $18^{th}$ was the highest. The strongest DPPH and ABTS radical scavenging activity was showed in S. nigrum fruit harvested on October $11^{th}$ and October $18^{th}$. The anti-inflammatory activity and antioxidant effects were the highest in the ethanol extract from S. nigrum fruit collected on October $18^{th}$ and October $11^{th}$. Thus, it seems the best to harvest of S. nigrum fruit harvested on October $11^{th}$ and October $18^{th}$.

키워드

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피인용 문헌

  1. 왕까마중(Solanum nigrum L.) 열매 분말의 추출 용매에 따른 항산화 및 항균 활성 vol.34, pp.2, 2017, https://doi.org/10.9799/ksfan.2021.34.2.137