Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Anti-Inflammatory Effect of 3-Bromo-4,5-Dihydroxybenzaldehyde, a Component of Polysiphonia morrowii, In Vivo and In Vitro

  • Kang, Na-Jin (Department of Medicine, School of Medicine, Jeju National University) ;
  • Han, Sang-Chul (Department of Medicine, School of Medicine, Jeju National University) ;
  • Kang, Hyun-Jae (Department of Medicine, School of Medicine, Jeju National University) ;
  • Ko, Geum (Department of Medicine, School of Medicine, Jeju National University) ;
  • Yoon, Weon-Jong (Jeju Biodiversity Research Institute (JBRI), Jeju Technopark (JTP)) ;
  • Kang, Hee-Kyoung (Department of Medicine, School of Medicine, Jeju National University) ;
  • Yoo, Eun-Sook (Department of Medicine, School of Medicine, Jeju National University)
  • Received : 2017.07.19
  • Accepted : 2017.09.14
  • Published : 2017.10.15
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Abstract

3-Bromo-4,5-dihydroxybenzaldehyde (BDB) is a natural bromophenol compound that is most commonly isolated from red algae. The present study was designed to investigate the anti-inflammatory properties of BDB on atopic dermatitis (AD) in mice induced by 2,4-dinitrochlorobenzene (DNCB) and on lipopolysaccharide (LPS)-stimulated murine macrophages. BDB treatment (100 mg/kg) resulted in suppression of the development of AD symptoms compared with the control treatment (induction-only), as demonstrated by reduced immunoglobulin E levels in serum, smaller lymph nodes with reduced thickness and length, a decrease in ear edema, and reduced levels of inflammatory cell infiltration in the ears. In RAW 264.7 murine macrophages, BDB (12.5, 25, 50, and $100{\mu}M$) suppressed the production of interleukin-6, a proinflammatory cytokine, in a dose-dependent manner. BDB also had an inhibitory effect on the phosphorylation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-${\kappa}B$) and signal transducer and activator of transcription 1 (STAT1; Tyr 701), two major signaling molecules involved in cellular inflammation. Taken together, the results show that BDB treatment alleviates inflammatory responses in an atopic dermatitis mouse model and RAW 264.7 macrophages. These results suggest that BDB may be a useful therapeutic strategy for treating conditions involving allergic inflammation such as atopic dermatitis.

Keywords

References

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