The Plant Pathology Journal

한국식물병리학회 (The Korean Society of Plant Pathology)
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Investigation of Genetic Diversity of Fusarium oxysporum f. sp. fragariae Using PCR-RFLP

  • Kim, Ji-Su (Division of Applied Life Science (BK21 Plus) and Institute of Agriculture & Life Sciences, Gyeongsang National University) ;
  • Kang, Nam Jun (Department of Agricultural Plant Science and Institute of Agriculture & Life Sciences, Gyeongsang National University) ;
  • Kwak, Youn-Sig (Division of Applied Life Science (BK21 Plus) and Institute of Agriculture & Life Sciences, Gyeongsang National University) ;
  • Lee, Choungkeun (R&D Coordination Division, Rural Development Administration)
  • 투고 : 2017.01.19
  • 심사 : 2017.02.20
  • 발행 : 2017.04.01
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초록

Fusarium wilts of strawberry, caused by Fusarium oxysporum f. sp. fragariae, is a serious soil-borne disease. Fusarium wilt causes dramatic yield losses in commercial strawberry production and it is a very stubborn disease to control. Reliable chemical control of strawberry Fusarium wilt disease is not yet available. Moreover, other well-known F. oxysporum have different genetic information from F. oxysporum f. sp. fragariae. This analysis investigates the genetic diversity of strawberry Fusairum wilt pathogen. In total, 110 pathogens were isolated from three major strawberry production regions, namely Sukok, Hadong, Sancheong in Gyeongnam province in South Korea. The isolates were confirmed using F. oxysporum f. sp. fragariae species-specific primer sets. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analyses were executed using the internal transcribed spacer, intergenic spacer, translation elongation factor1-${\alpha}$, and ${\beta}$-tubulin genes of the pathogens and four restriction enzymes: AluI, HhaI, HinP1I and HpyCH4V. Regarding results, there were diverse patterns in the three gene regions except for the ${\beta}$-tubulin gene region. Correlation analysis of strawberry cultivation region, cultivation method, variety, and phenotype of isolated pathogen, confirmed that genetic diversity depended on the classification of the cultivated region.

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참고문헌

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