The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

Efficient Transmission and Propagation of Tomato Chlorosis Virus by Simple Single-Leaflet Grafting

  • Lee, Huin (Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Kim, Mi-Kyeong (Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Choi, Hong-Soo (Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Kang, Jin-Ho (Department of International Agricultural Technology and Institutes of Green Bio Science and Technology, Seoul National University) ;
  • Ju, Ho-Jong (Department of Agricultural Biology, Chonbuk National University) ;
  • Seo, Jang-Kyun (Department of International Agricultural Technology and Institutes of Green Bio Science and Technology, Seoul National University)
  • Received : 2017.02.20
  • Accepted : 2017.03.06
  • Published : 2017.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

Tomato chlorosis virus (ToCV), a member of the genus Crinivirus, has caused an epidemic disease in tomato worldwide. ToCV is phloem-limited and transmitted by whiteflies in a semi-persistent manner, but not by mechanical inoculation. Experimental propagation of ToCV has been performed primarily by using whitefly-mediated inoculation. To develop a simple and convenient method for transmission of ToCV, we investigated grafting single-leaflets from tomato plants infected with ToCV to recipient tomato seedlings. Forty-one of 46 tomato seedlings tested were grafted successfully with single-leaflets infected with ToCV. Among them, 36 seedlings (87.8%) were systemically infected with ToCV and developed typical symptoms. Our results demonstrated that single-leaflet grafting could provide a sufficient amount of inoculum for the transmission of ToCV to the grafted seedlings.

Keywords

References

  1. Boyer, J. C. and Haenni, A. L. 1994. Infectious transcripts and cDNA clones of RNA viruses. Virology 198:415-426. https://doi.org/10.1006/viro.1994.1053
  2. Damsteegt, V. D., Scorza, R., Stone, A. L., Schneider, W. L., Webb, K., Demuth, M. and Gildow, F. E. 2007. Prunus host range of Plum pox virus (PPV) in the United States by aphid and graft inoculation. Plant Dis. 91:18-23. https://doi.org/10.1094/PD-91-0018
  3. Desbiez, C., Chandeysson, C., Lecoq, H. and Moury, B. 2012. A simple, rapid and efficient way to obtain infectious clones of potyviruses. J. Virol. Methods 183:94-97. https://doi.org/10.1016/j.jviromet.2012.03.035
  4. Drake, J. W. and Holland, J. J. 1999. Mutation rates among RNA viruses. Proc. Natl. Acad. Sci. U. S. A. 96:13910-13913. https://doi.org/10.1073/pnas.96.24.13910
  5. Hilf, M. E. and Lewis, R. S. 2016. Transmission and propagation of 'Candidatus Liberibacter asiaticus' by grafting with individual citrus leaves. Phytopathology 106:452-458. https://doi.org/10.1094/PHYTO-09-15-0221-R
  6. Kasrawi, M. A., Suwwan, M. A. and Mansour, A. 1988. Sources of resistance to tomato-yellow-leaf-curl-virus (TYLCV) in Lycopersicon species. Euphytica 37:61-64. https://doi.org/10.1007/BF00037224
  7. Liu, H. Y., Wisler, G. C. and Duffus, J. E. 2000. Particle lengths of whitefly-transmitted criniviruses. Plant Dis. 84:803-805. https://doi.org/10.1094/PDIS.2000.84.7.803
  8. Nie, X. and Singh, R. P. 2001. Differential accumulation of Potato virus A and expression of pathogenesis-related genes in resistant potato cv. Shepody upon graft inoculation. Phytopathology 91:197-203. https://doi.org/10.1094/PHYTO.2001.91.2.197
  9. Shi, B. J., Ding, S. W. and Symons, R. H. 1997. Plasmid vector for cloning infectious cDNAs from plant RNA viruses: high infectivity of cDNA clones of tomato aspermy cucumovirus. J. Gen. Virol. 78:1181-1185. https://doi.org/10.1099/0022-1317-78-5-1181
  10. Tromas, N. and Elena, S. F. 2010. The rate and spectrum of spontaneous mutations in a plant RNA virus. Genetics 185:983-989. https://doi.org/10.1534/genetics.110.115915
  11. Tzanetakis, I. E., Martin, R. R. and Wintermantel, W. M. 2013. Epidemiology of criniviruses: an emerging problem in world agriculture. Front. Microbiol. 4:119.
  12. Whitfield, A. E., Falk, B. W. and Rotenberg, D. 2015. Insect vector-mediated transmission of plant viruses. Virology 479-480:278-289. https://doi.org/10.1016/j.virol.2015.03.026
  13. Wintermantel, W. M. and Wisler, G. C. 2006. Vector specificity, host range, and genetic diversity of Tomato chlorosis virus. Plant Dis. 90:814-819. https://doi.org/10.1094/PD-90-0814
  14. Wintermantel, W. M., Wisler, G. C., Anchieta, A. G., Liu, H. Y., Karasev, A. V. and Tzanetakis, I. E. 2005. The complete nucleotide sequence and genome organization of tomato chlorosis virus. Arch. Virol. 150:2287-2298. https://doi.org/10.1007/s00705-005-0571-4