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First Report on Pink Rot of Sunflower (Helianthus annuus) Caused by Trichothecium roseum in Korea

Trichothecium roseum에 의한 해바라기 분홍빛썩음병 발생 보고

  • Kim, Sang Gyu (National Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Hur, On-Sook (National Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Sung, Jung Sook (National Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Ko, Ho-Cheol (National Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Luitel, Binod Prasad (National Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Rhee, Ju-Hee (National Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Baek, Hyung-Jin (National Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Ryu, Kyoung-Yul (National Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration)
  • 김상규 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 허온숙 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 성정숙 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 고호철 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • ;
  • 이주희 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 백형진 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 류경열 (농촌진흥청 국립농업과학원 농업유전자원센터)
  • Received : 2017.01.17
  • Accepted : 2017.03.28
  • Published : 2017.06.30

Abstract

Pink rot appeared in greenhouse-grown sunflower (Helianthus annuus L.) in Jeonju, Korea. The symptoms appeared as brown discoloration of sunflower head and progressed into stem in advanced stage. In order to investigate the causal organism of this disease, we isolated a fungus from the infected seeds and maintained the isolated fungal culture on potato dextrose agar medium. Conidiophores were simple or branched, 62.5 to $123.1{\mu}m$ long. Conidia were produced in basipetal chains, ellipsoidal to pyriform with oblique and prominent truncate basal scars, two-celled, hyaline and measured $10.2-21.4{\times}7.5-12.6{\mu}m$. The fungus was inoculated to a new sunflower plant and showed the typical blight on the leaves. Study of morphological characters, pathogenicity tests and sequence analysis revealed that the isolated fungus is confirmed to be Trichothecium roseum. To our knowledge, this is the first report of pink rot on sunflower caused by T. roseum in Korea.

해바라기 재배포장에서 화뢰 부분이 썩는 증상이 나타났다. 병징은 감염된 화뢰 부분이 갈변되어 줄기로 번져갔다. 감염된 화뢰의 종자에는 주황색의 포자덩어리를 관찰할 수 있었다. 병징으로부터 곰팡이를 순수 분리하여 감자 한천배지에 배양한 결과, conidiophores에 포자를 형성하여 흰색에서 분홍빛을 띠었다. Conidiophores는 단순형 또는 분지형으로 길이는 $62.5-123.1{\mu}m$였다. 분생포자는 무색의 격막이 없거나 하나 있는 둥근타원형에서 서양배 모양으로 크기는 $10.2-21.4{\times}7.5-12.6{\mu}m$였다. 이 균은 건전한 해바라기 잎에 접종하였을 때 잎에서 윤문형의 검은색 반점을 형성하였다. 균학적 특징, 병원성 검정, ITS 염기서열 분석 등의 결과를 바탕으로 Trichothecium roseum으로 동정되었으며 해바라기 분홍빛썩음병으로 명명하고자 한다.

Keywords

References

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