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Screening for Effective Organic Farming Materials for the Control of Cucumber Scab Caused by Cladosporium cucumerinum

오이 검은별무늬병 방제에 효과적인 유기농업자재 선발

  • Park, So-Hyang (Organic Agriculture Division, Department of Agricultural Environment, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Hong, Sung-Jun (Organic Agriculture Division, Department of Agricultural Environment, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Shim, Chang-Ki (Organic Agriculture Division, Department of Agricultural Environment, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Kim, Min-Jeong (Organic Agriculture Division, Department of Agricultural Environment, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Park, Jong-Ho (Organic Agriculture Division, Department of Agricultural Environment, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Han, Eun-Jung (Organic Agriculture Division, Department of Agricultural Environment, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Kim, Yong-Ki (Organic Agriculture Division, Department of Agricultural Environment, National Institute of Agricultural Sciences, Rural Development Administration)
  • 박소향 (농촌진흥청 국립농업과학원 농업환경부 유기농업과) ;
  • 홍성준 (농촌진흥청 국립농업과학원 농업환경부 유기농업과) ;
  • 심창기 (농촌진흥청 국립농업과학원 농업환경부 유기농업과) ;
  • 김민정 (농촌진흥청 국립농업과학원 농업환경부 유기농업과) ;
  • 박종호 (농촌진흥청 국립농업과학원 농업환경부 유기농업과) ;
  • 한은정 (농촌진흥청 국립농업과학원 농업환경부 유기농업과) ;
  • 김용기 (농촌진흥청 국립농업과학원 농업환경부 유기농업과)
  • Received : 2017.02.06
  • Accepted : 2017.04.02
  • Published : 2017.06.30

Abstract

This study was conducted to evaluate the control efficacy of the organic farming materials on cucumber scab caused by Cladosporium cucumerinum PT1 (KACC 48094). The antifungal activities in vitro as well as the suppressive effect of 43 organic farming materials on the spore germination and germ tube growth by inoculating spore suspension on cucumber seedlings in vivo were investigated. Thirteen organic farming materials inhibited the mycelial growth of C. cucumerinum and nine of these were microbial agents. In the screening using cucumber seedlings, six organic farming materials were very effective with control efficacy value of 90%. Among them, Bacillus amyloliquefaciens M27 provided suppressive effect on both mycelial growth and spore germination against cucumber scab. Finally, nine organic farming materials were selected to test the protective and curative effects, and all chosen organic farming materials significantly suppressed disease incidence when applied in the preventive action, in comparison with the curative action. Especially, Bordeaux mixture I and III gave excellent protective control efficacy with control values of 96.7% and 73.3%, respectively, whereas its curative control effect was significant low. Among these, only Thymus quinquecostatus+Sophora extract showed curative activity, although the control value was as low as 50%. This study suggests that cucumber scab can be controlled by some organic farming materials in the farmhouses under comparatively cold and wet condition and protective treatment is more important and efficient.

본 연구는 Cladosporium cucumerinum PT1 (KACC 48094)에 의해 발생하는 오이 검은별무늬병에 대하여 유기농업자재의 방제효과를 평가하기 위해 수행되었다. 43품목의 유기농업 자재를 대상으로 실내에서 항균활성 검정과 온실에서 오이 유묘에 포자현탁액 접종을 통한 유묘검정을 실시하였다. 13개 품목의 유기농업자재가 균사생장을 억제하였고, 이 중 9개 품목이 미생물 제제였다. 오이 유묘를 이용한 검정에서 6품목의 유기농업자재가 90% 이상의 우수한 방제효과를 나타냈다. 그 중에서 Bacillus amyloliquefaciens M27이 유효성분인 미생물 제제의 억제효과가 가장 뛰어났다. 그 다음으로 9개의 유기농업자재를 선발하여 예방적 및 치료적 효과를 알아보기 위해 적용하였는데, 예방적으로 처리했을 때 9개 자재 모두 병 발생을 상당히 억제하였다. 특히, 보르도액 I과 III은 방제가가 각각 96.7%, 73.3%로 훌륭한 예방효과를 나타냈다. 이 중 백리향+고삼 추출물이 함유된 자재에서만 방제가 가 50%로 다소 낮았지만 치료적 처리의 효과가 나타났다. 이 연구를 통해 시설 내 온화하고 습한 조건에서 잘 발생하는 오이 검은별무늬병은 유기농업자재로 방제될 수 있고 예방적 처리가 더욱 중요하고 효과적임이 확인되었다.

Keywords

References

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