Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Luteolin 5-O-glucoside from Korean Milk Thistle, Cirsium maackii, Exhibits Anti-Inflammatory Activity via Activation of the Nrf2/HO-1 Pathway

  • Jung, Hyun Ah (Department of Food Science and Human Nutrition, Chonbuk National University) ;
  • Roy, Anupom (Department of Food and Life Science, Pukyong National University) ;
  • Abdul, Qudeer Ahmed (Department of Food and Life Science, Pukyong National University) ;
  • Kim, Hyeung Rak (Department of Food and Life Science, Pukyong National University) ;
  • Park, Hee Juhn (Department of Pharmaceutical Engineering, Sangji University) ;
  • Choi, Jae Sue (Department of Food and Life Science, Pukyong National University)
  • Received : 2017.05.09
  • Accepted : 2017.05.26
  • Published : 2017.09.29
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Abstract

Luteolin 5-O-glucoside is the major flavonoid from Korean thistle, Cirsium maackii. We previously reported the anti-inflammatory activities of luteolin 5-O-glucoside in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. In this study, we determined the anti-inflammatory mechanisms of luteolin 5-O-glucoside through the inhibition of nitric oxide (NO) production in vitro and in vivo. Results revealed that luteolin 5-O-glucoside dose-dependently inhibited NO production and expression of iNOS and COX-2 in LPS-induced RAW 264.7 cells. Luteolin 5-O-glucoside also significantly inhibited the translocation of $NF-{\kappa}B$, the activation of MAPKs, and ROS generation in LPS-induced RAW 264.7 cells. In addition, protein expressions of Nrf-2 and HO-1 were also upregulated by luteolin 5-O-glucoside treatment. Moreover, luteolin 5-O-glucoside inhibited ${\lambda}-carrageenan-induced$ mouse paw edema by 65.34% and 48.31% at doses of 50 and 100 mg/kg body weight, respectively. These findings indicate potential anti-inflammatory effect of luteolin 5-O-glucoside particularly by downregulating $NF-{\kappa}B$ and upregulating HO-1/Nrf-2 pathway.

Keywords

References

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