Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Anti-proliferative and Antioxidant Activities of 1-methoxy-3-methyl-8-hydroxy-anthraquinone, a Hydroxyanthraquinoid Extrolite Produced by Amycolatopsis thermoflava strain SFMA-103

  • Kumar, C. Ganesh (Medicinal Chemistry and Biotechnology Division, CSIR-Indian Institute of Chemical Technology) ;
  • Mongolla, Poornima (Medicinal Chemistry and Biotechnology Division, CSIR-Indian Institute of Chemical Technology) ;
  • Chandrasekhar, Cheemalamarri (Medicinal Chemistry and Biotechnology Division, CSIR-Indian Institute of Chemical Technology) ;
  • Poornachandra, Yedla (Medicinal Chemistry and Biotechnology Division, CSIR-Indian Institute of Chemical Technology) ;
  • Siva, Bandi (Natural Products Chemistry Division, CSIR-Indian Institute of Chemical Technology) ;
  • Babu, K. Suresh (Natural Products Chemistry Division, CSIR-Indian Institute of Chemical Technology) ;
  • Ramakrishna, Kallaganti Venkata Siva (Nuclear Magnetic Resonance Centre, CSIR-Indian Institute of Chemical Technology)
  • Received : 2017.05.04
  • Accepted : 2017.06.26
  • Published : 2017.09.28
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Abstract

Actinobacteria are prolific producers of a large number of natural products with diverse biological activities. In the present study, an actinobacterium isolated from sunflower rhizosphere soil sample collected from Medak, Andhra Pradesh, South India was identified as Amycolatopsis thermoflava strain SFMA-103. A pigmented secondary metabolite in culture broth was extracted by using methanol and it was further purified by silica gel column chromatography with methanol-chloroform solvent system. Structural elucidation studies based on UV-visible, 1D and 2D-NMR, FT-IR, and mass spectroscopic analyses confirmed the structure as 1-methoxy-3-methyl-8-hydroxy-anthraquinone. It showed significant in vitro anticancer activity against lung cancer and lymphoblastic leukemia cells with $IC_{50}$ values of 10.3 and $16.98{\mu}M$, respectively. In addition, 1-methoxy-3-methyl-8-hydroxy-anthraquinone showed good free radical scavenging activity by DPPH method with an $EC_{50}$ of $18.2{\mu}g/ml$. It also showed other promising superoxide radical scavenging, nitric oxide radical scavenging and inhibition of lipid peroxidation activities. This is a first report of anti-proliferative and antioxidant activities of 1-methoxy-3-methyl-8-hydroxy-anthraquinone isolated from A. thermoflava strain SFMA-103 which may find potential application in biotechnological and pharmaceutical fields.

Keywords

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