생명과학회지 (Journal of Life Science)

  • 제27권9호
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  • Pages.975-985
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  • 2017
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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UV조사에 의해 유발된 SKH-1 hairless 마우스의 피부노화에 미치는 한천마스크의 개선효과

Beneficial Effect of an Agar Mask against Skin Damage Induced by UV Exposure in SKH-1 Hairless Mice

  • 송보람 (부산대학교 생명자원과학대학 바이오소재과학과 & 생명융합연구센터) ;
  • 김지은 (부산대학교 생명자원과학대학 바이오소재과학과 & 생명융합연구센터) ;
  • 윤우빈 (부산대학교 생명자원과학대학 바이오소재과학과 & 생명융합연구센터) ;
  • 이미림 (부산대학교 생명자원과학대학 바이오소재과학과 & 생명융합연구센터) ;
  • 최준영 (부산대학교 생명자원과학대학 바이오소재과학과 & 생명융합연구센터) ;
  • 박진주 (부산대학교 생명자원과학대학 바이오소재과학과 & 생명융합연구센터) ;
  • 김동섭 (부산대학교 생명자원과학대학 식품공학과) ;
  • 이충열 ((주)강림오가닉) ;
  • 이희섭 (부산대학교 생활환경대학 식품영양학과) ;
  • 임용 (동의대학교 의료보건생활대학 임상병리학과) ;
  • 정민욱 ((주) 밀양한천) ;
  • 김배환 (계명대학교 공중보건학과) ;
  • 황대연 (부산대학교 생명자원과학대학 바이오소재과학과 & 생명융합연구센터)
  • Song, Bo Ram (Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University) ;
  • Kim, Ji Eun (Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University) ;
  • Yun, Woo Bin (Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University) ;
  • Lee, Mi Rim (Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University) ;
  • Choi, Jun Young (Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University) ;
  • Park, Jin Ju (Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University) ;
  • Kim, Dong Seob (Department of Food Science & Technology, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University) ;
  • Lee, Chung Yeoul (Gangrim Organics) ;
  • Lee, Hee Seob (Department of Food Science & Nutrition, College of Human Ecology, Pusan National University) ;
  • Lim, Yong (Department of Clinical Laboratory Science, College of Nursing and Healthcare Science, Dong-Eui University) ;
  • Jung, Min Wook (Research Center, Miryang Agar-Agar Co., LTD) ;
  • Kim, Bae Hwan (Department of Public Health, Keimyung University) ;
  • Hwang, Dae Youn (Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University)
  • 투고 : 2017.06.05
  • 심사 : 2017.08.17
  • 발행 : 2017.09.30
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초록

UV 조사에 의해 유도된 광노화(photoaging)에 대한 한천젤마스크(agar gel mask, AGM)의 효과를 평가하기 위해, SKH-1 hairless 마우스의 피부에 4주간의 UV조사와 AGM 및 에센스처리 한천젤마스크(agar gel mask dipped in essence, AGMdE)를 동시에 처리한 후 피부주름, 피부표현형, 피부조직구조, 항산화조건 및 독성 등을 분석하였다. AGM과 AGMdE의 부착은 주름형성의 억제, 홍반지수의 감소, 피부수분의 증진뿐만 아니라 경피 수분손실(TEWL)을 방지하였다. 또한, 표피두께는 UV+Vehicle 처리군과 비교했을 때, UV+AGM 및 UV+AGMdE 처리군에서 UV를 조사하지 않은 그룹과 유사한 수준으로 회복되었다. MMP-1과 tyrosinase의 발현수준은 UV+Vehicle 처리군에서 가장 높게 증가하였으나 UV+AGM 및 UV+AGMdE 처리군에서 유의적으로 감소하였다. 더불어, SOD 활성은 UV+Vehicle 처리군에 비하여 UV+AGM 및 UV+AGMdE 처리군에서 유의적으로 감소되었지만, 대부분의 독성화합물 처리에 의해 유도되는 혈청생화학적 지표와 조직학적 특성의 변화는 UV+AGM 및 UV+AGMdE 처리군에서 관찰되지 않았다. 따라서, 이러한 결과들은 AGM이 피부형태, 조직학적 구조, 산화적 상태를 조절함으로써 피부노화를 예방하는 마스크팩으로 활용될 수 있는 가능성을 있음을 제시한다.

To investigate the beneficial effects of an agar gel mask (AGM) on UV-induced photoaging, SKH-1 hairless mice were treated with a topical application of AGM and an AGM dipped in essence (AGMdE). The mice were divided into an no radiation group, UV + AGM, UV + AGMdE, and UV + vehicle (PBS) treatment groups. Alterations in skin wrinkles, skin phenotype, histological structures, oxidative status, and toxicity were then evaluated during 4 weeks of exposure. The topical application of AGM and AGMdE inhibited wrinkle formation, suppressed the erythema index, prevented transepidermal water loss, and enhanced skin hydration. In addition, epidermal thickness recovered to a similar level as that in the no irradiation group in the UV + AGM and UV + AGMdE treatment groups compared with the UV + vehicle (distilled water) group. Furthermore, the expression levels of matrix metalloproteinase-1 (MMP-1) and tyrosinase were reduced in the UV + AGM and UV + AGMdE treatment groups, although the highest level varied. Moreover, superoxide dismutase (SOD) activity was significantly lower in the UV + AGM and UV + AGMdE treatment groups as compared with the UV + vehicle group. No significant alterations induced by most toxic compounds were measured in serum biochemical markers and liver and kidney histological features of the UV + AGM and UV + AGMdE treatment groups. These results suggest that AGM may protect against skin aging by regulating skin morphology, histopathological structures, and oxidative conditions.

키워드

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