생명과학회지 (Journal of Life Science)

  • 제27권9호
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  • Pages.986-993
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  • 2017
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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LPS로 유도된 RAW 264.7 세포에 대한 레몬 머틀 잎 추출물의 항염증 효과

Anti-inflammatory Effects of Lemon Myrtle (Backhousia citriodora) Leaf Extracts in LPS-induced RAW 264.7 Cells

  • 김판길 (경남과학기술대학교 제약공학과) ;
  • 정경임 (신라대학교 식품영양학과) ;
  • 최영주 (신라대학교 식품영양학과) ;
  • 갈상완 (경남과학기술대학교 제약공학과)
  • Kim, Pan Kil (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology) ;
  • Jung, Kyung Im (Department of Food & Nutrition, Silla University) ;
  • Choi, Young Ju (Department of Food & Nutrition, Silla University) ;
  • Gal, Sang Wan (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology)
  • 투고 : 2017.06.09
  • 심사 : 2017.08.24
  • 발행 : 2017.09.30
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초록

본 연구에서는 레몬 머틀 잎 열수 및 에탄올 추출물을 이용하여 항산화 활성과 NO 소거능 및 RAW 264.7 세포에서의 항염증 활성에 미치는 영향을 확인하였다. 레몬 머틀 열수 및 에탄올 추출물의 총 페놀 함량은 각각 207.44와 $331.54{\mu}g\;TAE/mg$이었고, DPPH radical 소거능은 0.5 mg/ml 농도에서 각각 90.69%와 92.50%로 나타났으며, SOD 활성은 1 mg/ml 농도에서 각각 106.22%와 103.58%로 나타났다. 아질산염 소거능 분석에서는 열수와 에탄올 추출물 1 mg/ml 농도, pH 1.2에서 81.06%와 91.03%로 에탄올 추출물의 소거능이 높은 것으로 나타났다. 레몬머틀의 항염증 활성을 측정하기 위하여 대식세포에 LPS를 처리하여 세포 내 NO 생성을 유도한 후 열수 및 에탄올 추출물을 처리하여 각각의 추출물이 NO 활성에 미치는 영향을 분석한 결과 각 추출물 모두 농도 의존적으로 감소하였다. 또한 LPS에 의해 증가된 iNOS 단백질 발현 또한 농도 의존적으로 감소하였다. 이상의 결과에서와 같이 레몬 머틀 잎은 항산화 및 항염증 효과가 있는 것으로 나타났기에 천연물 소재로서의 활용도가 높을 것으로 생각된다.

Lemon myrtle (Backhousia citriodora) has been identified as one of the plants that are likely to undergo important commercial exploitation. This study was carried out to investigate the anti-inflammatory activities and nitric oxide synthase (iNOS) expression of hot water (LMW) and 80% ethanol (LME) extracts from lemon myrtle leaf in lipopolysaccharide-induced (LPS) RAW 264.7 cells. The total phenol content of LMW and LME was 207.44 and $331.54{\mu}g$ tannic acid equivalents (TAE)/mg, respectively (p<0.01). DPPH radical scavenging activities of LMW and LME were remarkably increased in a dose-dependent manner, and were about 90.69% and 92.50% at 0.5 mg/ml, respectively. Superoxide dismutase (SOD) activities of LMW and LME were 106.22% and 103.58% at 1 mg/ml, respectively. The highest activity (91.03%) of nitrite-scavenging was observed for LME at 1 mg/ml at pH 1.2, while the activity for LMW was about 81.03% under the same conditions (p<0.05). Anti-inflammatory effect was examined in LPS stimulated RAW 264.7 cells. Nitric oxide (NO) production were reduced to 35.41% and 78.39% by addition of LMW and LME at 0.5 mg/ml, respectively (p<0.05). LMW and LME reduced protein expression of inducible nitric oxide synthase (iNOS) in a dose-dependent manner (p<0.05). These results, we conclude that lemon myrtle may be a highly valuable natural product owing to its high-quality functional components as well as its anti-oxidant and anti-inflammatory activities.

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