Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Protective Effects of Pyrrosiae Folium on the 2% Glucose-Induced Toxicity in Caenorhabditis elegans

석위가 예쁜꼬마선충에서 Glucose로 유도된 독성에 미치는 영향

  • 김봉석 (우석대학교 약학대학) ;
  • 이병주 (우석대학교 약학대학) ;
  • 이현주 (우석대학교 약학대학) ;
  • 안순영 (우석대학교 약학대학) ;
  • 박지원 (우석대학교 약학대학) ;
  • 윤선화 (우석대학교 약학대학) ;
  • 오미진 (한국식품연구원) ;
  • 권진 (한국복지대학 의료보장구과) ;
  • 이세연 (우석대학교 약학대학) ;
  • 차동석 (우석대학교 약학대학) ;
  • 오찬호 (우석대학교 식품생명공학과) ;
  • 전훈 (우석대학교 약학대학)
  • Received : 2017.08.08
  • Accepted : 2017.09.11
  • Published : 2017.09.30
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Abstract

Pyrrosia lingua which belongs to Polypodiaceae has been used as a traditional medicine for the treatment of urinary system inflammation, urination disorder, and bronchitis. However, there are not enough phytochemical and pharmacological studies of P. lingua up to now. Here in this study, the protective effect of MeOH extract of whole plant of Pyrrosia lingua (MPL) against 2% glucose-induced toxicity was investigated using Caenorhabditis elegans (C. elegans) model system. We found that MPL significantly extended the lifespan of wild-type nematode under normal culture condition. MPL also effectively recovered the decreased lifespan caused by 2% glucose-toxicity. In addition, MPL efficiently attenuated the increased glucose concentration inside of nematode. Further studies evaluating diabetes-related factors revealed that MPL reduced both intracellular ROS and lipid accumulation which were up-regulated under 2% glucose supplement condition. Our data also showed that MPL improved the 2% glucose-induced shortened body movement of nematode. Lastly, we carried out genetic studies using several single gene knockout mutants to establish the possible target of MPL. Our results demonstrated that genes such as daf-2 and daf-16 were responsible for the protective activity of MPL against 2% glucose-induced toxicity. These results indicate that MPL exerts protective action against 2% glucose via regulation of insulin/IGF-1 sinaling pathway and FOXO activation.

Keywords

References

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